CHAC1 Antibody - #DF9353

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Product: CHAC1 Antibody
Catalog: DF9353
Description: Rabbit polyclonal antibody to CHAC1
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 29kDa; 24kD(Calculated).
Uniprot: Q9BUX1
RRID: AB_2842549

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
CHAC1 Antibody detects endogenous levels of total CHAC1.
RRID:
AB_2842549
Cite Format: Affinity Biosciences Cat# DF9353, RRID:AB_2842549.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Blocks Notch protein; Botch; Cation transport regulator like protein 1; Cation transport regulator-like protein 1; ChaC; ChaC cation transport regulator homolog 1; ChaC cation transport regulator like 1; chac1; CHAC1_HUMAN; Gamma GCT acting on glutathione homolog 1; Gamma-GCG 1; Glutathione-specific gamma-glutamylcyclotransferase 1;

Immunogens

Immunogen:
Uniprot:

Q9BUX1(CHAC1_HUMAN) >>Visit HPA database.

Gene(ID):
CHAC1(79094)
Sequence:
MKQESAAPNTPPTSQSPTPSAQFPRNDGDPQALWIFGYGSLVWRPDFAYSDSRVGFVRGYSRRFWQGDTFHRGSDKMPGRVVTLLEDHEGCTWGVAYQVQGEQVSKALKYLNVREAVLGGYDTKEVTFYPQDAPDQPLKALAYVATPQNPGYLGPAPEEAIATQILACRGFSGHNLEYLLRLADFMQLCGPQAQDEHLAAIVDAVGTMLPCFCPTEQALALV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
83
Chicken
83
Zebrafish
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BUX1 As Substrate

Site PTM Type Enzyme
S61 Phosphorylation
T92 Phosphorylation
K109 Sumoylation
K109 Ubiquitination
K124 Ubiquitination
S172 Phosphorylation

Research Backgrounds

Function:

Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos.

Subcellular Location:

Cytoplasm>Cytosol. Golgi apparatus>trans-Golgi network.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with NOTCH1 (via extracellular region).

Family&Domains:

Belongs to the gamma-glutamylcyclotransferase family. ChaC subfamily.

References

1). Identification of Ferroptotic Genes in Spinal Cord Injury at Different Time Points: Bioinformatics and Experimental Validation. Molecular Neurobiology, 2022 (PubMed: 35796899) [IF=5.1]
2). CPEB1 enhances erastin‐induced ferroptosis in gastric cancer cells by suppressing twist1 expression. IUBMB LIFE, 2021 (PubMed: 34184391) [IF=4.6]

Application: WB    Species: Mice    Sample: GC cells

FIGURE 4 CPEB1 suppressed twist1 expression in GC cells. (a-f) After CPEB1-silenced AGS cells and CPEB1-overexpressed HGC-27 cells were treated with erastin (10 μg/ml) for 24 h, twist1, ATF4, and CHAC1 protein expression levels were measured by western blot. N = 3. Data are shown as means ± SD. **p < 0.01, and ***p < 0.001. ATF4, activating transcription factor 4; CPEB1, cytoplasmic polyadenylation element binding protein 1; CHAC1, ChaC glutathione-specific γ glutamylcyclotransferase 1; GC, gastric cancer

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