CATSPER4 Antibody | Affinity Biosciences

IF/ICC
Cites

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Figure 4 The effect of Olfr25 knockdown on calcium ion levels and the expression of CatSper channel genes in GC-2 cells.

Product:	CATSPER4 Antibody
Catalog:	DF9351
Description:	Rabbit polyclonal antibody to CATSPER4
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Pig, Horse, Sheep, Rabbit
Mol.Wt.:	50~60kDa; 54kD(Calculated).
Uniprot:	Q7RTX7
RRID:	AB_2842547

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(88%), Horse(88%), Sheep(88%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

CATSPER4 Antibody detects endogenous levels of total CATSPER4.

RRID:

AB_2842547
Cite Format: Affinity Biosciences Cat# DF9351, RRID:AB_2842547.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cation channel sperm associated 4; Cation channel sperm associated protein 4; CatSper4; Channel sperm associated 4; Ion channel CatSper4; MGC149223;

Immunogens

Immunogen:

Uniprot:

Q7RTX7(CTSR4_HUMAN) >>Visit HPA database.

Gene(ID):

CATSPER4(378807)

Expression:

Q7RTX7 CTSR4_HUMAN:

Testis-specific.

Sequence:

Q7RTX7 CTSR4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRDNEKAWWQQWTSHTGLEGWGGTQEDRMGFGGAVAALRGRPSPLQSTIHESYGRPEEQVLINRQEITNKADAWDMQEFITHMYIKQLLRHPAFQLLLALLLVINAITIALRTNSYLDQKHYELFSTIDDIVLTILLCEVLLGWLNGFWIFWKDGWNILNFIIVFILLLRFFINEINIPSINYTLRALRLVHVCMAVEPLARIIRVILQSVPDMANIMVLILFFMLVFSVFGVTLFGAFVPKHFQNIQVALYTLFICITQDGWVDIYSDFQTEKREYAMEIGGAIYFTIFITIGAFIGINLFVIVVTTNLEQMMKAGEQGQQQRITFSETGAEEEEENDQLPLVHCVVARSEKSGLLQEPLAGGPLSNLSENTCDNFCLVLEAIQENLRQYKEIRDELNMIVEEVRAIRFNQEQESEVLNRRSSTSGSLETTSSKDIRQMSQQQDLLSALVSMEKVHDSSSQILLKKHKSSH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Sheep

Bovine

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7RTX7 As Substrate

Q7RTX7

Site	PTM Type	Source
S423	Phosphorylation	Uniprot
S424	Phosphorylation	Uniprot
K466	Ubiquitination	Uniprot

Research Backgrounds

Q7RTX7_CTSR4_HUMAN

Function:

Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte.

Subcellular Location:

Cell projection>Cilium>Flagellum membrane>Multi-pass membrane protein.
Note: Specifically located in the principal piece of sperm tail.

Tissue Specificity:

Testis-specific.

Subunit Structure:

Heterotetramer; possibly composed of CATSPER1, CATSPER2, CATSPER3 and CATSPER4 (Potential). Component of the CatSper complex. Interacts with CATSPER1 (By similarity).

Family&Domains:

Belongs to the cation channel sperm-associated (TC 1.A.1.19) family.

References

1). The Olfactory Receptor Olfr25 Mediates Sperm Dysfunction Induced by Low-Dose Bisphenol A through the CatSper-Ca2+ Signaling Pathway. Toxics, 2024 (PubMed: 38922122) [IF=4.6]

Application: WB Species: Mouse Sample: GC-2 cells

Figure 4 The effect of Olfr25 knockdown on calcium ion levels and the expression of CatSper channel genes in GC-2 cells. (A) RT-qPCR was used to measure the mRNA expression levels of Olfr25 (n = 3). (B) Intracellular calcium ion concentration was detected after Olfr25 knockdown (n = 3). (C) The mRNA expression levels of Catsper1–4 in GC-2 cells after Olfr25 knockdown were detected using qRT-PCR (n = 3). (D) The protein expression levels of CatSper1–4 in GC-2 cells after Olfr25 knockdown were detected using Western blot analyses. (E) Quantitative analysis of the indicated proteins using Image J software. All data are presented as the mean ± SD. ns, not significant. ** p < 0.01.

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CATSPER4 Antibody - #DF9351