CATSPER1 Antibody - #DF9349

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Product: CATSPER1 Antibody
Catalog: DF9349
Description: Rabbit polyclonal antibody to CATSPER1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Sheep, Rabbit
Mol.Wt.: 90 kDa; 90kD(Calculated).
Uniprot: Q8NEC5
RRID: AB_2842545

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Sheep(83%), Rabbit(80%)
Clonality:
Polyclonal
Specificity:
CATSPER1 Antibody detects endogenous levels of total CATSPER1.
RRID:
AB_2842545
Cite Format: Affinity Biosciences Cat# DF9349, RRID:AB_2842545.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cation channel sperm associated 1; Cation channel sperm associated protein 1; Cation channel sperm-associated protein 1; CATSPER 1; CATSPER; CatSper1; CTSR1_HUMAN; hCatSper; MGC33335; MGC33368; Sperm associated cation channel 1; Sperm ion channel;

Immunogens

Immunogen:
Uniprot:

Q8NEC5(CTSR1_HUMAN) >>Visit HPA database.

Gene(ID):
CATSPER1(117144)
Expression:
Q8NEC5 CTSR1_HUMAN:

Testis-specific.

Sequence:
MDQNSVPEKAQNEADTNNADRFFRSHSSPPHHRPGHSRALHHYELHHHGVPHQRGESHHPPEFQDFHDQALSSHVHQSHHHSEARNHGRAHGPTGFGLAPSQGAVPSHRSYGEDYHDELQRDGRRHHDGSQYGGFHQQSDSHYHRGSHHGRPQYLGENLSHYSSGVPHHGEASHHGGSYLPHGPNPYSESFHHSEASHLSGLQHDESQHHQVPHRGWPHHHQVHHHGRSRHHEAHQHGKSPHHGETISPHSSVGSYQRGISDYHSEYHQGDHHPSEYHHGDHPHHTQHHYHQTHRHRDYHQHQDHHGAYHSSYLHGDYVQSTSQLSIPHTSRSLIHDAPGPAASRTGVFPYHVAHPRGSAHSMTRSSSTIRSRVTQMSKKVHTQDISTKHSEDWGKEEGQFQKRKTGRLQRTRKKGHSTNLFQWLWEKLTFLIQGFREMIRNLTQSLAFETFIFFVVCLNTVMLVAQTFAEVEIRGEWYFMALDSIFFCIYVVEALLKIIALGLSYFFDFWNNLDFFIMAMAVLDFLLMQTHSFAIYHQSLFRILKVFKSLRALRAIRVLRRLSFLTSVQEVTGTLGQSLPSIAAILILMFTCLFLFSAVLRALFRKSDPKRFQNIFTTIFTLFTLLTLDDWSLIYMDSRAQGAWYIIPILVIYIIIQYFIFLNLVITVLVDSFQTALFKGLEKAKQERAARIQEKLLEDSLTELRAAEPKEVASEGTMLKRLIEKKFGTMTEKQQELLFHYLQLVASVEQEQQKFRSQAAVIDEIVDTTFEAGEEDFRN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
83
Rabbit
80
Pig
0
Horse
0
Bovine
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NEC5 As Substrate

Site PTM Type Enzyme
K396 Acetylation
K403 Acetylation

Research Backgrounds

Function:

Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte.

Subcellular Location:

Cell projection>Cilium>Flagellum membrane>Multi-pass membrane protein.
Note: Specifically located in the principal piece of sperm tail.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Testis-specific.

Subunit Structure:

Heterotetramer; possibly composed of CATSPER1, CATSPER2, CATSPER3 and CATSPER4 (Potential). Component of the CatSper complex. Interacts with CATSPER3 and CATSPER4 (By similarity). Interacts with Ca(v)3.3/CACNA1I, leading to suppress T-type calcium channel activity.

Family&Domains:

Belongs to the cation channel sperm-associated (TC 1.A.1.19) family.

References

1). Homozygous mutation in SLO3 leads to severe asthenoteratozoospermia due to acrosome hypoplasia and mitochondrial sheath malformations. Reproductive Biology and Endocrinology, 2022 (PubMed: 34980136) [IF=4.4]

Application: WB    Species: Human    Sample: sperm cells

Fig. 6 Membrane potential and expression patterns of LRRC52 and CatSper1 examined in the spermatozoa from control and SLO3-mutant subjects. A Membrane potential measurements of wild-type and SLO3 mutant sperm in capacitated conditions. The capacitation-associated hyperpolarization of human sperm is inhibited in SLO3 mutant spermatozoa, Data are presented as the mean ± SEM. ***P < 0.001; Student’s t-test. B Immunoblotting of sperm lysates from controls and the SLO3-mutated individual using the anti-LRRC52 and CatSper1 antibodies. Na+/K+-ATPase α1 was used as loading control. The expression of the LRRC52 protein is decreased in mutant sperm, whereas the level of the CatSper1 protein is comparable to that of control. C Representative images of spermatozoa from fertile controls and the SLO3 mutant patient stained with the anti-LRRC52 antibody, anti-CatSper1 antibody, anti-Ac-tubulin antibody, and Hoechst. Two independent experiments were performed and at least 150 sperm we re-examined for each time per individual. Scale bars, 20 μm

Application: IF/ICC    Species: Human    Sample: sperm cells

Fig. 6 Membrane potential and expression patterns of LRRC52 and CatSper1 examined in the spermatozoa from control and SLO3-mutant subjects. A Membrane potential measurements of wild-type and SLO3 mutant sperm in capacitated conditions. The capacitation-associated hyperpolarization of human sperm is inhibited in SLO3 mutant spermatozoa, Data are presented as the mean ± SEM. ***P < 0.001; Student’s t-test. B Immunoblotting of sperm lysates from controls and the SLO3-mutated individual using the anti-LRRC52 and CatSper1 antibodies. Na+/K+-ATPase α1 was used as loading control. The expression of the LRRC52 protein is decreased in mutant sperm, whereas the level of the CatSper1 protein is comparable to that of control. C Representative images of spermatozoa from fertile controls and the SLO3 mutant patient stained with the anti-LRRC52 antibody, anti-CatSper1 antibody, anti-Ac-tubulin antibody, and Hoechst. Two independent experiments were performed and at least 150 sperm we re-examined for each time per individual. Scale bars, 20 μm

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