CALR3 Antibody - #DF9324
| Product: | CALR3 Antibody |
| Catalog: | DF9324 |
| Description: | Rabbit polyclonal antibody to CALR3 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Sheep, Rabbit, Dog |
| Mol.Wt.: | 45 kDa; 45kD(Calculated). |
| Uniprot: | Q96L12 |
| RRID: | AB_2842520 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9324, RRID:AB_2842520.
Fold/Unfold
CALR 3; CALR3; CALR3_HUMAN; Calreticulin 2; Calreticulin-2; Calreticulin-3; Calreticulin2; Calreticulin3; Calsperin; Cancer/testis antigen 93; CMH19; CRT2; CT93; FLJ25355; MGC26577;
Immunogens
- Q96L12 CALR3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARALVQLWAICMLRVALATVYFQEEFLDGEHWRNRWLQSTNDSRFGHFRLSSGKFYGHKEKDKGLQTTQNGRFYAISARFKPFSNKGKTLVIQYTVKHEQKMDCGGGYIKVFPADIDQKNLNGKSQYYIMFGPDICGFDIKKVHVILHFKNKYHENKKLIRCKVDGFTHLYTLILRPDLSYDVKIDGQSIESGSIEYDWNLTSLKKETSPAESKDWEQTKDNKAQDWEKHFLDASTSKQSDWNGDLDGDWPAPMLQKPPYQDGLKPEGIHKDVWLHRKMKNTDYLTQYDLSEFENIGAIGLELWQVRSGTIFDNFLITDDEEYADNFGKATWGETKGPEREMDAIQAKEEMKKAREEEEEELLSGKINRHEHYFNQFHRRNEL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96L12 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y57 | Phosphorylation | Uniprot | |
| K89 | Acetylation | Uniprot | |
| T209 | Phosphorylation | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| K230 | Acetylation | Uniprot | |
| Y374 | Phosphorylation | Uniprot |
Research Backgrounds
During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility (By similarity). CALR3 capacity for calcium-binding may be absent or much lower than that of CALR.
Endoplasmic reticulum lumen.
Testis specific.
Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5.
Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Belongs to the calreticulin family.
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