CNN3 Antibody - #DF9323

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Product: CNN3 Antibody
Catalog: DF9323
Description: Rabbit polyclonal antibody to CNN3
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 36 kDa; 36kD(Calculated).
Uniprot: Q15417
RRID: AB_2842519

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Bovine(100%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
CNN3 Antibody detects endogenous levels of total CNN3.
RRID:
AB_2842519
Cite Format: Affinity Biosciences Cat# DF9323, RRID:AB_2842519.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Acidic calponin 3; acidic isoform; Calponin 3 acidic; Calponin 3; Calponin acidic; Calponin acidic isoform; Calponin; Calponin-3; Calponin3; CNN 3; Cnn3; CNN3_HUMAN; dJ639P13.2.2; OTTHUMP00000012470; OTTHUMP00000012471;

Immunogens

Immunogen:
Uniprot:

Q15417(CNN3_HUMAN) >>Visit HPA database.

Gene(ID):
CNN3(1266)
Expression:
Q15417 CNN3_HUMAN:

Expressed in both non-smooth muscle tissues as well as smooth muscle tissues.

Sequence:
MTHFNKGPSYGLSAEVKNKIASKYDHQAEEDLRNWIEEVTGMSIGPNFQLGLKDGIILCELINKLQPGSVKKVNESSLNWPQLENIGNFIKAIQAYGMKPHDIFEANDLFENGNMTQVQTTLVALAGLAKTKGFHTTIDIGVKYAEKQTRRFDEGKLKAGQSVIGLQMGTNKCASQAGMTAYGTRRHLYDPKMQTDKPFDQTTISLQMGTNKGASQAGMLAPGTRRDIYDQKLTLQPVDNSTISLQMGTNKVASQKGMSVYGLGRQVYDPKYCAAPTEPVIHNGSQGTGTNGSEISDSDYQAEYPDEYHGEYQDDYPRDYQYSDQGIDY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Sheep
92
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15417 As Substrate

Site PTM Type Enzyme
T2 Phosphorylation
K6 Acetylation
K6 Ubiquitination
S9 Phosphorylation
Y10 Phosphorylation P12931 (SRC)
S13 Phosphorylation
K23 Acetylation
K23 Ubiquitination
S43 Phosphorylation
C59 S-Nitrosylation
K156 Acetylation
K158 Methylation
K158 Ubiquitination
S162 Phosphorylation
K172 Methylation
K172 Ubiquitination
C173 S-Nitrosylation
S175 Phosphorylation
Y182 Phosphorylation
T184 Phosphorylation
K197 Ubiquitination
S205 Phosphorylation
K212 Ubiquitination
S215 Phosphorylation
K232 Ubiquitination
T234 Phosphorylation
T242 Phosphorylation
S244 Phosphorylation
K251 Ubiquitination
S254 Phosphorylation
K256 Ubiquitination
S259 Phosphorylation
Y261 Phosphorylation
S285 Phosphorylation
S293 Phosphorylation O75116 (ROCK2)
S296 Phosphorylation
Y300 Phosphorylation
Y308 Phosphorylation
Y316 Phosphorylation
Y320 Phosphorylation
Y322 Phosphorylation
S323 Phosphorylation

Research Backgrounds

Function:

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

Tissue Specificity:

Expressed in both non-smooth muscle tissues as well as smooth muscle tissues.

Family&Domains:

Belongs to the calponin family.

References

1). Promoting effects of calponin 3 on the growth of diffuse large B‑cell lymphoma cells. Oncology Reports, 2023 (PubMed: 36660952) [IF=4.2]

Application: WB    Species: Human    Sample: DLBCL cells

Figure 2. - Overexpression and knockdown of CNN3 in DLBCL cells. The changes in the expression of CNN3 in DB and SU-DHL-4 cells were confirmed using (A and B) reverse transcription-quantitative PCR and (C) western blot analysis following transfection with CNN3 overexpression vector or siRNA. ***P

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