Product: CAPN7 Antibody
Catalog: DF9322
Description: Rabbit polyclonal antibody to CAPN7
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 93kDa; 93kD(Calculated).
Uniprot: Q9Y6W3
RRID: AB_2842518

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
CAPN7 Antibody detects endogenous levels of total CAPN7.
RRID:
AB_2842518
Cite Format: Affinity Biosciences Cat# DF9322, RRID:AB_2842518.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Calpain like protease; Calpain7; CAPN 7; Homolog of Aspergillus Nidulans PALB; PALBH;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9Y6W3 CAN7_HUMAN:

Ubiquitous.

Sequence:
MDATALERDAVQFARLAVQRDHEGRYSEAVFYYKEAAQALIYAEMAGSSLENIQEKITEYLERVQALHSAVQSKSADPLKSKHQLDLERAHFLVTQAFDEDEKENVEDAIELYTEAVDLCLKTSYETADKVLQNKLKQLARQALDRAEALSEPLTKPVGKISSTSVKPKPPPVRAHFPLGANPFLERPQSFISPQSCDAQGQRYTAEEIEVLRTTSKINGIEYVPFMNVDLRERFAYPMPFCDRWGKLPLSPKQKTTFSKWVRPEDLTNNPTMIYTVSSFSIKQTIVSDCSFVASLAISAAYERRFNKKLITGIIYPQNKDGEPEYNPCGKYMVKLHLNGVPRKVIIDDQLPVDHKGELLCSYSNNKSELWVSLIEKAYMKVMGGYDFPGSNSNIDLHALTGWIPERIAMHSDSQTFSKDNSFRMLYQRFHKGDVLITASTGMMTEAEGEKWGLVPTHAYAVLDIREFKGLRFIQLKNPWSHLRWKGRYSENDVKNWTPELQKYLNFDPRTAQKIDNGIFWISWDDLCQYYDVIYLSWNPGLFKESTCIHSTWDAKQGPVKDAYSLANNPQYKLEVQCPQGGAAVWVLLSRHITDKDDFANNREFITMVVYKTDGKKVYYPADPPPYIDGIRINSPHYLTKIKLTTPGTHTFTLVVSQYEKQNTIHYTVRVYSACSFTFSKIPSPYTLSKRINGKWSGQSAGGCGNFQETHKNNPIYQFHIEKTGPLLIELRGPRQYSVGFEVVTVSTLGDPGPHGFLRKSSGDYRCGFCYLELENIPSGIFNIIPSTFLPKQEGPFFLDFNSIIPIKITQLQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
88
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6W3 As Substrate

Site PTM Type Enzyme
M1 Acetylation
Y42 Phosphorylation
K74 Ubiquitination
S75 Phosphorylation
K80 Ubiquitination
K82 Ubiquitination
T95 Phosphorylation
K130 Ubiquitination
K135 Ubiquitination
K156 Acetylation
K156 Ubiquitination
K167 Ubiquitination
S190 Phosphorylation
K217 Ubiquitination
K247 Ubiquitination
S251 Phosphorylation
K253 Ubiquitination
K309 Ubiquitination
K320 Ubiquitination
K331 Ubiquitination
K344 Ubiquitination
K356 Ubiquitination
S412 Phosphorylation
S414 Phosphorylation
T416 Phosphorylation
K419 Ubiquitination
K477 Ubiquitination
Y489 Phosphorylation
S490 Phosphorylation
K495 Ubiquitination
K503 Ubiquitination
K556 Ubiquitination
K596 Ubiquitination
K617 Ubiquitination
Y619 Phosphorylation
Y620 Phosphorylation
Y627 Phosphorylation
S635 Phosphorylation
K641 Ubiquitination
Y659 Phosphorylation
Y667 Phosphorylation
K695 Ubiquitination
S697 Phosphorylation
S700 Phosphorylation
K712 Ubiquitination
K723 Ubiquitination

Research Backgrounds

Function:

Calcium-regulated non-lysosomal thiol-protease.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Family&Domains:

Belongs to the peptidase C2 family.

Restrictive clause

 

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