CALML5 Antibody | Affinity Biosciences

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Western blot analysis of CALML5 using MCF7 whole cell lysates.

Product:	CALML5 Antibody
Catalog:	DF9316
Description:	Rabbit polyclonal antibody to CALML5
Application:	WB IHC
Reactivity:	Human, Rat
Prediction:	Pig, Bovine, Sheep, Dog
Mol.Wt.:	16 kDa; 16kD(Calculated).
Uniprot:	Q9NZT1
RRID:	AB_2842512

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Pig(100%), Bovine(100%), Sheep(100%), Dog(88%)

Clonality:

Polyclonal

Specificity:

CALML5 Antibody detects endogenous levels of total CALML5.

RRID:

AB_2842512
Cite Format: Affinity Biosciences Cat# DF9316, RRID:AB_2842512.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CALL5_HUMAN; CALML5; Calmodulin like 5; Calmodulin-like protein 5; Calmodulin-like skin protein; CLSP;

Immunogens

Immunogen:

Uniprot:

Q9NZT1(CALL5_HUMAN) >>Visit HPA database.

Gene(ID):

CALML5(51806)

Expression:

Q9NZT1 CALL5_HUMAN:

Particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation. Very low expression in lung.

Sequence:

Q9NZT1 CALL5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGELTPEEEAQYKKAFSAVDTDGNGTINAQELGAALKATGKNLSEAQLRKLISEVDSDGDGEISFQEFLTAAKKARAGLEDLQVAFRAFDQDGDGHITVDELRRAMAGLGQPLPQEELDAMIREADVDQDGRVNYEEFARMLAQE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

Horse

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NZT1 As Substrate

Q9NZT1

Site	PTM Type	Source
T6	Phosphorylation	Uniprot
Y13	Phosphorylation	Uniprot
S18	Phosphorylation	Uniprot
T22	Phosphorylation	Uniprot
T27	Phosphorylation	Uniprot
S45	Phosphorylation	Uniprot
S58	Phosphorylation	Uniprot
S65	Phosphorylation	Uniprot
Y136	Phosphorylation	Uniprot

Research Backgrounds

Q9NZT1_CALL5_HUMAN

Function:

Binds calcium. May be involved in terminal differentiation of keratinocytes.

Tissue Specificity:

Particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation. Very low expression in lung.

Subunit Structure:

Associates with transglutaminase 3.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Glioma. (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Sensory system > Olfactory transduction.

· Organismal Systems > Sensory system > Phototransduction.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

References

1). AHNAK, regulated by the OSM/OSMR signaling, involved in the development of primary localized cutaneous amyloidosis. Journal of Dermatological Science, 2023 (PubMed: 37100691) [IF=4.6]

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CALML5 Antibody - #DF9316