ATP2C2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from MCF7 cells(serum starvation treatment), using ATP2C2 Antibody.

Product:	ATP2C2 Antibody
Catalog:	DF9312
Description:	Rabbit polyclonal antibody to ATP2C2
Application:	WB
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Dog
Mol.Wt.:	103 kDa; 103kD(Calculated).
Uniprot:	O75185
RRID:	AB_2842508

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(88%), Bovine(88%), Horse(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

ATP2C2 Antibody detects endogenous levels of total ATP2C2.

RRID:

AB_2842508
Cite Format: Affinity Biosciences Cat# DF9312, RRID:AB_2842508.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AT2C2_HUMAN; ATP2C2; ATPase 2C2; ATPase, Ca++ transporting, type 2C, member 2; Calcium-transporting ATPase type 2C member 2; DKFZp686H22230; hSPCA2; KIAA0703; PMR1; Secretory pathway Ca(2+)-ATPase 2; secretory pathway calcium ATPase 2;

Immunogens

Immunogen:

Uniprot:

O75185(AT2C2_HUMAN) >>Visit HPA database.

Gene(ID):

ATP2C2(9914)

Sequence:

O75185 AT2C2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVEGRVSEFLKKLGFSGGGRQYQALEKDEEEALIDEQSELKAIEKEKKVTALPPKEACKCQKEDLARAFCVDLHTGLSEFSVTQRRLAHGWNEFVADNSEPVWKKYLDQFKNPLILLLLGSALVSVLTKEYEDAVSIATAVLVVVTVAFIQEYRSEKSLEELTKLVPPECNCLREGKLQHLLARELVPGDVVSLSIGDRIPADIRLTEVTDLLVDESSFTGEAEPCSKTDSPLTGGGDLTTLSNIVFMGTLVQYGRGQGVVIGTGESSQFGEVFKMMQAEETPKTPLQKSMDRLGKQLTLFSFGIIGLIMLIGWSQGKQLLSMFTIGVSLAVAAIPEGLPIVVMVTLVLGVLRMAKKRVIVKKLPIVETLGCCSVLCSDKTGTLTANEMTVTQLVTSDGLRAEVSGVGYDGQGTVCLLPSKEVIKEFSNVSVGKLVEAGCVANNAVIRKNAVMGQPTEGALMALAMKMDLSDIKNSYIRKKEIPFSSEQKWMAVKCSLKTEDQEDIYFMKGALEEVIRYCTMYNNGGIPLPLTPQQRSFCLQEEKRMGSLGLRVLALASGPELGRLTFLGLVGIIDPPRVGVKEAVQVLSESGVSVKMITGDALETALAIGRNIGLCNGKLQAMSGEEVDSVEKGELADRVGKVSVFFRTSPKHKLKIIKALQESGAIVAMTGDGVNDAVALKSADIGIAMGQTGTDVSKEAANMILVDDDFSAIMNAVEEGKGIFYNIKNFVRFQLSTSISALSLITLSTVFNLPSPLNAMQILWINIIMDGPPAQSLGVEPVDKDAFRQPPRSVRDTILSRALILKILMSAAIIISGTLFIFWKEMPEDRASTPRTTTMTFTCFVFFDLFNALTCRSQTKLIFEIGFLRNHMFLYSVLGSILGQLAVIYIPPLQRVFQTENLGALDLLFLTGLASSVFILSELLKLCEKYCCSPKRVQMHPEDV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Pig

Bovine

Sheep

Xenopus

Chicken

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75185 As Substrate

O75185

Site	PTM Type	Source
T146	Phosphorylation	Uniprot
Y153	Phosphorylation	Uniprot
T264	Phosphorylation	Uniprot
S267	Phosphorylation	Uniprot
S268	Phosphorylation	Uniprot
S290	Phosphorylation	Uniprot
S329	Phosphorylation	Uniprot
T346	Phosphorylation	Uniprot
K363	Acetylation	Uniprot
K380	Acetylation	Uniprot
T457	Phosphorylation	Uniprot
S471	Phosphorylation	Uniprot
S487	Phosphorylation	Uniprot
T600	Phosphorylation	Uniprot
T606	Phosphorylation	Uniprot
K634	Ubiquitination	Uniprot

Research Backgrounds

O75185_AT2C2_HUMAN

Function:

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Family&Domains:

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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ATP2C2 Antibody - #DF9312