CABIN1 Antibody - #DF9294

(2)  
Product: CABIN1 Antibody
Catalog: DF9294
Description: Rabbit polyclonal antibody to CABIN1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Rabbit, Dog
Mol.Wt.: 246kDa; 246kD(Calculated).
Uniprot: Q9Y6J0
RRID: AB_2842490

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CABIN1 Antibody detects endogenous levels of total CABIN1.
RRID:
AB_2842490
Cite Format: Affinity Biosciences Cat# DF9294, RRID:AB_2842490.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A330070M20Rik; Cabin 1; CABIN_HUMAN; Cabin1; CAIN; Calcineurin binding protein cabin 1; Calcineurin inhibitor; Calcineurin-binding protein cabin-1; KIAA0330; PPP3IN;

Immunogens

Immunogen:
Uniprot:

Q9Y6J0(CABIN_HUMAN) >>Visit HPA database.

Gene(ID):
CABIN1(23523)
Expression:
Q9Y6J0 CABIN_HUMAN:

Widely expressed in different tissues.

Sequence:
MIRIAALNASSTIEDDHEGSFKSHKTQTKEAQEAEAFALYHKALDLQKHDRFEESAKAYHELLEASLLREAVSSGDEKEGLKHPGLILKYSTYKNLAQLAAQREDLETAMEFYLEAVMLDSTDVNLWYKIGHVALRLIRIPLARHAFEEGLRCNPDHWPCLDNLITVLYTLSDYTTCLYFICKALEKDCRYSKGLVLKEKIFEEQPCLRKDSLRMFLKCDMSIHDVSVSAAETQAIVDEALGLRKKRQALIVREKEPDLKLVQPIPFFTWKCLGESLLAMYNHLTTCEPPRPSLGKRIDLSDYQDPSQPLESSMVVTPVNVIQPSTVSTNPAVAVAEPVVSYTSVATTSFPLHSPGLLETGAPVGDISGGDKSKKGVKRKKISEESGETAKRRSARVRNTKCKKEEKVDFQELLMKFLPSRLRKLDPEEEDDSFNNYEVQSEAKLESFPSIGPQRLSFDSATFMESEKQDVHEFLLENLTNGGILELMMRYLKAMGHKFLVRWPPGLAEVVLSVYHSWRRHSTSLPNPLLRDCSNKHIKDMMLMSLSCMELQLDQWLLTKGRSSAVSPRNCPAGMVNGRFGPDFPGTHCLGDLLQLSFASSQRDLFEDGWLEFVVRVYWLKARFLALQGDMEQALENYDICTEMLQSSTAIQVEAGAERRDIVIRLPNLHNDSVVSLEEIDKNLKSLERCQSLEEIQRLYEAGDYKAVVHLLRPTLCTSGFDRAKHLEFMTSIPERPAQLLLLQDSLLRLKDYRQCFECSDVALNEAVQQMVNSGEAAAKEEWVATVTQLLMGIEQALSADSSGSILKVSSSTTGLVRLTNNLIQVIDCSMAVQEEAKEPHVSSVLPWIILHRIIWQEEDTFHSLCHQQQLQNPAEEGMSETPMLPSSLMLLNTAHEYLGRRSWCCNSDGALLRFYVRVLQKELAASTSEDTHPYKEELETALEQCFYCLYSFPSKKSKARYLEEHSAQQVDLIWEDALFMFEYFKPKTLPEFDSYKTSTVSADLANLLKRIATIVPRTERPALSLDKVSAYIEGTSTEVPCLPEGADPSPPVVNELYYLLADYHFKNKEQSKAIKFYMHDICICPNRFDSWAGMALARASRIQDKLNSNELKSDGPIWKHATPVLNCFRRALEIDSSNLSLWIEYGTMSYALHSFASRQLKQWRGELPPELVQQMEGRRDSMLETAKHCFTSAARCEGDGDEEEWLIHYMLGKVAEKQQQPPTVYLLHYRQAGHYLHEEAARYPKKIHYHNPPELAMEALEVYFRLHASILKLLGKPDSGVGAEVLVNFMKEAAEGPFARGEEKNTPKASEKEKACLVDEDSHSSAGTLPGPGASLPSSSGPGLTSPPYTATPIDHDYVKCKKPHQQATPDDRSQDSTAVALSDSSSTQDFFNEPTSLLEGSRKSYTEKRLPILSSQAGATGKDLQGATEERGKNEESLESTEGFRAAEQGVQKPAAETPASACIPGKPSASTPTLWDGKKRGDLPGEPVAFPQGLPAGAEEQRQFLTEQCIASFRLCLSRFPQHYKSLYRLAFLYTYSKTHRNLQWARDVLLGSSIPWQQLQHMPAQGLFCERNKTNFFNGIWRIPVDEIDRPGSFAWHMNRSIVLLLKVLAQLRDHSTLLKVSSMLQRTPDQGKKYLRDADRQVLAQRAFILTVKVLEDTLSELAEGSERPGPKVCGLPGARMTTDVSHKASPEDGQEGLPQPKKPPLADGSGPGPEPGGKVGLLNHRPVAMDAGDSADQSGERKDKESPRAGPTEPMDTSEATVCHSDLERTPPLLPGRPARDRGPESRPTELSLEELSISARQQPTPLTPAQPAPAPAPATTTGTRAGGHPEEPLSRLSRKRKLLEDTESGKTLLLDAYRVWQQGQKGVAYDLGRVERIMSETYMLIKQVDEEAALEQAVKFCQVHLGAAAQRQASGDTPTTPKHPKDSRENFFPVTVVPTAPDPVPADSVQRPSDAHTKPRPALAAATTIITCPPSASASTLDQSKDPGPPRPHRPEATPSMASLGPEGEELARVAEGTSFPPQEPRHSPQVKMAPTSSPAEPHCWPAEAALGTGAEPTCSQEGKLRPEPRRDGEAQEAASETQPLSSPPTAASSKAPSSGSAQPPEGHPGKPEPSRAKSRPLPNMPKLVIPSAATKFPPEITVTPPTPTLLSPKGSISEETKQKLKSAILSAQSAANVRKESLCQPALEVLETSSQESSLESETDEDDDYMDI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
100
Rabbit
100
Pig
91
Horse
73
Xenopus
67
Chicken
56
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6J0 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
S11 Phosphorylation
T12 Phosphorylation
S20 Phosphorylation
K29 Ubiquitination
K42 Ubiquitination
K48 Ubiquitination
K57 Ubiquitination
S66 Phosphorylation
S74 Phosphorylation
K89 Ubiquitination
K193 Ubiquitination
K198 Ubiquitination
K200 Ubiquitination
K260 Ubiquitination
S386 Phosphorylation
K424 Ubiquitination
S433 Phosphorylation
Y437 Phosphorylation
K444 Ubiquitination
S447 Phosphorylation
S450 Phosphorylation
T480 Phosphorylation
Y491 Phosphorylation
S673 Phosphorylation
K685 Ubiquitination
K706 Ubiquitination
Y753 Phosphorylation
S802 Phosphorylation
S830 Phosphorylation
Y948 Phosphorylation
Y951 Phosphorylation
K997 Ubiquitination
T1014 Phosphorylation
K1113 Ubiquitination
T1148 Phosphorylation
K1162 Ubiquitination
K1188 Ubiquitination
K1277 Ubiquitination
S1388 Phosphorylation
S1398 Phosphorylation
S1439 Phosphorylation
S1442 Phosphorylation
T1474 Phosphorylation
S1605 Phosphorylation
K1677 Ubiquitination
S1695 Phosphorylation
S1740 Phosphorylation
S1744 Phosphorylation
S1752 Phosphorylation
T1767 Phosphorylation
S1771 Phosphorylation
T1776 Phosphorylation
T1795 Phosphorylation
S1798 Phosphorylation
S1803 Phosphorylation
T1811 Phosphorylation
T1814 Phosphorylation
R1831 Methylation
S1855 Phosphorylation
K1857 Ubiquitination
Y1864 Phosphorylation
Y1876 Phosphorylation
K1906 Ubiquitination
T1924 Phosphorylation
T1926 Phosphorylation
T1927 Phosphorylation
S1934 Phosphorylation
S2035 Phosphorylation
S2067 Phosphorylation
T2089 Phosphorylation
S2094 Phosphorylation
T2142 Phosphorylation
T2149 Phosphorylation
T2151 Phosphorylation
T2154 Phosphorylation
T2156 Phosphorylation
S2159 Phosphorylation
S2165 Phosphorylation
K2173 Methylation
S2181 Phosphorylation

Research Backgrounds

Function:

May be required for replication-independent chromatin assembly. May serve as a negative regulator of T-cell receptor (TCR) signaling via inhibition of calcineurin. Inhibition of activated calcineurin is dependent on both PKC and calcium signals. Acts as a negative regulator of p53/TP53 by keeping p53 in an inactive state on chromatin at promoters of a subset of it's target genes.

PTMs:

Activated through PKC-mediated hyperphosphorylation. Phosphorylation by the DNA damage kinases ATM and CHK2 enhances ubiquitination.

Upon genotoxic stress, ubiquitination by the DCX(DDB2) E3 ubiquitin-protein ligase complex targets CABIN1 for proteasomal degradation, leading to the release of p53/TP53.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed in different tissues.

Subunit Structure:

Component of a complex that includes at least ASF1A, CABIN1, HIRA, histone H3.3 and UBN1. Interacts with calcineurin. Interacts with MEF2B.

Restrictive clause

 

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