BCCIP Antibody - #DF9290
| Product: | BCCIP Antibody |
| Catalog: | DF9290 |
| Description: | Rabbit polyclonal antibody to BCCIP |
| Application: | WB IF/ICC |
| Reactivity: | Human |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 36 kDa; 36kD(Calculated). |
| Uniprot: | Q9P287 |
| RRID: | AB_2842486 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9290, RRID:AB_2842486.
Fold/Unfold
bccip; BCCIP_HUMAN; BCCIPalpha; BCCIPbeta; BRCA2 and CDKN1A interacting protein; BRCA2 and CDKN1A-interacting protein; BRCA2 and Cip1/p21 interacting protein; Cdk inhibitor p21 binding protein; P21 and CDK associated protein 1; P21- and CDK-associated protein 1; Protein TOK-1; Protein TOK1; TOK 1; TOK 1alpha; TOK 1beta; TOK1;
Immunogens
Expressed at high levels in testis and skeletal muscle and at lower levels in brain, heart, kidney, liver, lung, ovary, pancreas, placenta, and spleen.
- Q9P287 BCCIP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASRSKRRAVESGVPQPPDPPVQRDEEEEKEVENEDEDDDDSDKEKDEEDEVIDEEVNIEFEAYSLSDNDYDGIKKLLQQLFLKAPVNTAELTDLLIQQNHIGSVIKQTDVSEDSNDDMDEDEVFGFISLLNLTERKGTQCVEQIQELVLRFCEKNCEKSMVEQLDKFLNDTTKPVGLLLSERFINVPPQIALPMYQQLQKELAGAHRTNKPCGKCYFYLLISKTFVEAGKNNSKKKPSNKKKAALMFANAEEEFFYEKAILKFNYSVQEESDTCLGGKWSFDDVPMTPLRTVMLIPGDKMNEIMDKLKEYLSV
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9P287 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R7 | Methylation | Uniprot | |
| R8 | Methylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| T109 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| K235 | Acetylation | Uniprot | |
| T288 | Phosphorylation | Uniprot |
Research Backgrounds
During interphase, required for microtubule organizing and anchoring activities. During mitosis, required for the organization and stabilization of the spindle pole. Isoform 2/alpha is particularly important for the regulation of microtubule anchoring, microtubule stability, spindle architecture and spindle orientation, compared to isoform 1/beta. May promote cell cycle arrest by enhancing the inhibition of CDK2 activity by CDKN1A. May be required for repair of DNA damage by homologous recombination in conjunction with BRCA2. May not be involved in non-homologous end joining (NHEJ).
Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cytoplasm>Cytoskeleton>Spindle pole.
Note: Colocalizes with BRCA2 in discrete nuclear foci (PubMed:15713648). In interphase, preferential localizes to the mother centriole (PubMed:28394342). Recruited to the spindle pole matrix and centrosome by microtubules and dynein/dynactin activity (PubMed:28394342).
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle pole.
Note: Isoform 1/beta tends to be less abundant at, and less strongly associated with, centrosomes than isoform 2/alpha.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle pole.
Note: Isoform 2/alpha tends to be more abundant at, and more strongly associated with, centrosomes than isoform 1/beta.
Expressed at high levels in testis and skeletal muscle and at lower levels in brain, heart, kidney, liver, lung, ovary, pancreas, placenta, and spleen.
Interacts with BRCA2, CDKN1A and MTDH/LYRIC. Isoform 2/alpha, but not isoform 1/beta, interacts with DCTN1/p150-glued and ACTR1A/ARP1. Both isoform 1 and isoform 2 interact with alpha-, beta- and gamma-tubulins. Interacts with TENT5C; the interaction has no effect on TENT5C poly(A) polymerase function.
Belongs to the BCP1 family.
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