BATF2 Antibody | Affinity Biosciences

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Cites

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Western blot analysis of extracts from Hela cells, using BATF2 Antibody.

Figure 5 Circ_0005927 sponged miR-942-5p to regulate BATF2.

Product:	BATF2 Antibody
Catalog:	DF9266
Description:	Rabbit polyclonal antibody to BATF2
Application:	WB
Reactivity:	Human
Prediction:	Pig, Horse, Sheep, Dog
Mol.Wt.:	29kDa; 29kD(Calculated).
Uniprot:	Q8N1L9
RRID:	AB_2842462

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(91%), Horse(82%), Sheep(91%), Dog(90%)

Clonality:

Polyclonal

Specificity:

BATF2 Antibody detects endogenous levels of total BATF2.

RRID:

AB_2842462
Cite Format: Affinity Biosciences Cat# DF9266, RRID:AB_2842462.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

B ATF 2; Basic leucine zipper transcriptional factor ATF like 2; BATF2; MGC20410; SARI; Suppressor of AP 1 regulated by IFN;

Immunogens

Immunogen:

Uniprot:

Q8N1L9(BATF2_HUMAN) >>Visit HPA database.

Gene(ID):

BATF2(116071)

Sequence:

Q8N1L9 BATF2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MHLCGGNGLLTQTDPKEQQRQLKKQKNRAAAQRSRQKHTDKADALHQQHESLEKDNLALRKEIQSLQAELAWWSRTLHVHERLCPMDCASCSAPGLLGCWDQAEGLLGPGPQGQHGCREQLELFQTPGSCYPAQPLSPGPQPHDSPSLLQCPLPSLSLGPAVVAEPPVQLSPSPLLFASHTGSSLQGSSSKLSALQPSLTAQTAPPQPLELEHPTRGKLGSSPDNPSSALGLARLQSREHKPALSAATWQGLVVDPSPHPLLAFPLLSSAQVHF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Sheep

Dog

Horse

Rabbit

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N1L9 As Substrate

Q8N1L9

Site	PTM Type	Enzyme	Source
S227	Phosphorylation		Uniprot

Research Backgrounds

Q8N1L9_BATF2_HUMAN

Function:

AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system. Following infection, participates in the differentiation of CD8(+) thymic conventional dendritic cells in the immune system. Acts via the formation of a heterodimer with JUN family proteins that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3' and regulates expression of target genes (By similarity). Selectively suppresses CCN1 transcription and hence blocks the downstream cell proliferation signals produced by CCN1 and inhibits CCN1-induced anchorage-independent growth and invasion in several cancer types, such as breast cancer, malignant glioma and metastatic melanoma. Possibly acts by interfering with AP-1 binding to CCN1 promoter.

Subcellular Location:

Nucleus.

Subunit Structure:

Heterodimer; heterodimerizes with JUN family proteins.

Family&Domains:

Belongs to the bZIP family.

References

1). Circ_0005927 Inhibits the Progression of Colorectal Cancer by Regulating miR-942-5p/BATF2 Axis. Cancer Management and Research, 2021 (PubMed: 33732022) [IF=3.3]

Application: WB Species: Human Sample: NCM460, LoVo and SW620 cells

Figure 5 Circ_0005927 sponged miR-942-5p to regulate BATF2. (A) The binding sites between miR-942-5p and BATF2 were predicted by TargetScan online database. (B and C) Dual-luciferase reporter assay was performed to verify that miR-942-5p bound to BATF2. (D) Western blot was carried out to detect the protein expression of BATF2 in NCM460, LoVo and SW620 cells. (E) QRT-PCR was employed to determine BATF2 mRNA expression in CRC tissues and normal intestinal tissues. (F and G) The linear relationship between BATF2 expression and circ_0005927 expression or miR-942-5p expression was explained by Spearman correlation analysis. (H) The interfering efficiency of anti-miR-942-5p was determined by qRT-PCR. (I) The effect of miR-942-5p inhibitor on the protein expression of BATF2 was disclosed by Western blot. (J) The impacts between circ_0005927 overexpression and miR-942-5p mimic on BATF2 protein expression were unveiled by Western blot. **P<0.01.

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BATF2 Antibody - #DF9266