BAGE5 Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using BAGE5 Antibody.

Product:	BAGE5 Antibody
Catalog:	DF9265
Description:	Rabbit polyclonal antibody to BAGE5
Application:	WB
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	51 kDa; 51kD(Calculated).
Uniprot:	Q9UL15
RRID:	AB_2842461

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Pig(91%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

BAGE5 Antibody detects endogenous levels of total BAGE5.

RRID:

AB_2842461
Cite Format: Affinity Biosciences Cat# DF9265, RRID:AB_2842461.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BAG family molecular chaperone regulator 5; BAG-5; BAG5; BAG5_HUMAN; Bcl 2 associated athanogene 5; Bcl-2-associated athanogene 5; BCL2 associated athanogene 5; KIAA0873;

Immunogens

Immunogen:

Uniprot:

Q9UL15(BAG5_HUMAN) >>Visit HPA database.

Gene(ID):

BAG5(9529)

Sequence:

Q9UL15 BAG5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDMGNQHPSISRLQEIQKEVKSVEQQVIGFSGLSDDKNYKKLERILTKQLFEIDSVDTEGKGDIQQARKRAAQETERLLKELEQNANHPHRIEIQNIFEEAQSLVREKIVPFYNGGNCVTDEFEEGIQDIILRLTHVKTGGKISLRKARYHTLTKICAVQEIIEDCMKKQPSLPLSEDAHPSVAKINFVMCEVNKARGVLIALLMGVNNNETCRHLSCVLSGLIADLDALDVCGRTEIRNYRREVVEDINKLLKYLDLEEEADTTKAFDLRQNHSILKIEKVLKRMREIKNELLQAQNPSELYLSSKTELQGLIGQLDEVSLEKNPCIREARRRAVIEVQTLITYIDLKEALEKRKLFACEEHPSHKAVWNVLGNLSEIQGEVLSFDGNRTDKNYIRLEELLTKQLLALDAVDPQGEEKCKAARKQAVRLAQNILSYLDLKSDEWEY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Pig

Xenopus

Chicken

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UL15 As Substrate

Q9UL15

Site	PTM Type	Source
K18	Ubiquitination	Uniprot
K21	Ubiquitination	Uniprot
S22	Phosphorylation	Uniprot
K37	Ubiquitination	Uniprot
T47	Phosphorylation	Uniprot
K48	Ubiquitination	Uniprot
K61	Ubiquitination	Uniprot
K80	Ubiquitination	Uniprot
K108	Ubiquitination	Uniprot
K138	Ubiquitination	Uniprot
K142	Ubiquitination	Uniprot
K169	Ubiquitination	Uniprot
Y241	Phosphorylation	Uniprot
K251	Ubiquitination	Uniprot
K254	Ubiquitination	Uniprot
K266	Ubiquitination	Uniprot
K278	Acetylation	Uniprot
K278	Ubiquitination	Uniprot
K290	Ubiquitination	Uniprot
K307	Ubiquitination	Uniprot
K356	Ubiquitination	Uniprot
K393	Ubiquitination	Uniprot
K404	Ubiquitination	Uniprot
K419	Ubiquitination	Uniprot
Y437	Phosphorylation	Uniprot

Research Backgrounds

Q9UL15_BAG5_HUMAN

Function:

Inhibits both auto-ubiquitination of PRKN and ubiquitination of target proteins by PRKN (By similarity). May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding.

Subunit Structure:

Binds to the ATPase domain of HSP/HSC70 chaperones. Binds PRKN.

Family&Domains:

The fifth BAG domain is responsible for the interaction with HSP70 nucleotide-binding domain.

Restrictive clause

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For Research Use Only.
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BAGE5 Antibody - #DF9265