DDX18 Antibody - #DF9258
(1)
(3)
| Product: | DDX18 Antibody |
| Catalog: | DF9258 |
| Description: | Rabbit polyclonal antibody to DDX18 |
| Application: | WB IF/ICC |
| Reactivity: | Human |
| Prediction: | Bovine, Sheep |
| Mol.Wt.: | 75kDa; 75kD(Calculated). |
| Uniprot: | Q9NVP1 |
| RRID: | AB_2842454 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Prediction:
Bovine(89%), Sheep(89%)
Clonality:
Polyclonal
Specificity:
DDX18 Antibody detects endogenous levels of total DDX18.
RRID:
AB_2842454
Cite Format: Affinity Biosciences Cat# DF9258, RRID:AB_2842454.
Cite Format: Affinity Biosciences Cat# DF9258, RRID:AB_2842454.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
ATP dependent RNA helicase DDX18; ATP-dependent RNA helicase DDX18; DDX 18; DDX18; DDX18_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 18; DEAD box polypeptide 18; DEAD box protein 18; FLJ33908; MrDb; Myc regulated DEAD box protein; Myc-regulated DEAD box protein;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- Q9NVP1 DDX18_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSHLPMKLLRKKIEKRNLKLRQRNLKFQGASNLTLSETQNGDVSEETMGSRKVKKSKQKPMNVGLSETQNGGMSQEAVGNIKVTKSPQKSTVLTNGEAAMQSSNSESKKKKKKKRKMVNDAEPDTKKAKTENKGKSEEESAETTKETENNVEKPDNDEDESEVPSLPLGLTGAFEDTSFASLCNLVNENTLKAIKEMGFTNMTEIQHKSIRPLLEGRDLLAAAKTGSGKTLAFLIPAVELIVKLRFMPRNGTGVLILSPTRELAMQTFGVLKELMTHHVHTYGLIMGGSNRSAEAQKLGNGINIIVATPGRLLDHMQNTPGFMYKNLQCLVIDEADRILDVGFEEELKQIIKLLPTRRQTMLFSATQTRKVEDLARISLKKEPLYVGVDDDKANATVDGLEQGYVVCPSEKRFLLLFTFLKKNRKKKLMVFFSSCMSVKYHYELLNYIDLPVLAIHGKQKQNKRTTTFFQFCNADSGTLLCTDVAARGLDIPEVDWIVQYDPPDDPKEYIHRVGRTARGLNGRGHALLILRPEELGFLRYLKQSKVPLSEFDFSWSKISDIQSQLEKLIEKNYFLHKSAQEAYKSYIRAYDSHSLKQIFNVNNLNLPQVALSFGFKVPPFVDLNVNSNEGKQKKRGGGGGFGYQKTKKVEKSKIFKHISKKSSDSRQFSH
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Bovine
89
Sheep
89
Horse
75
Xenopus
67
Dog
63
Rabbit
63
Pig
58
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NVP1 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| K7 | Acetylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| T47 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| K57 | Acetylation | Uniprot | |
| K59 | Acetylation | Uniprot | |
| T68 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| K82 | Acetylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| T91 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| K126 | Acetylation | Uniprot | |
| K135 | Sumoylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| T225 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| T230 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| T260 | Phosphorylation | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| T319 | Phosphorylation | Uniprot | |
| Y324 | Phosphorylation | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| K352 | Acetylation | Uniprot | |
| T360 | Phosphorylation | Uniprot | |
| T368 | Phosphorylation | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| Y385 | Phosphorylation | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| Y440 | Phosphorylation | Uniprot | |
| Y442 | Phosphorylation | Uniprot | |
| Y447 | Phosphorylation | Uniprot | |
| K458 | Acetylation | Uniprot | |
| K545 | Acetylation | Uniprot | |
| K545 | Ubiquitination | Uniprot | |
| S549 | Phosphorylation | Uniprot | |
| K557 | Ubiquitination | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| K571 | Acetylation | Uniprot | |
| K571 | Ubiquitination | Uniprot | |
| K577 | Acetylation | Uniprot | |
| K577 | Ubiquitination | Uniprot | |
| K584 | Ubiquitination | Uniprot | |
| R588 | Methylation | Uniprot | |
| S627 | Phosphorylation | Uniprot | |
| R635 | Methylation | Uniprot | |
| Y643 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Probable RNA-dependent helicase.
Subcellular Location:
Nucleus>Nucleolus. Chromosome.
Subunit Structure:
Interacts with NOL8; the interaction is RNA-dependent.
Family&Domains:
Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.
References
1). DDX18 Facilitates the Tumorigenesis of Lung Adenocarcinoma by Promoting Cell Cycle Progression through the Upregulation of CDK4. International journal of molecular sciences, 2024
(PubMed: 38732173)
[IF=5.6]
Application: WB Species: Human Sample: H157 and H460 cells
Figure 2 DDX18 Upregulation in LUAD Tissues and its Functional Role in LUAD Cells. (a,b) Heatmaps depicting global mRNA expression profiles in GSE75037 [(a) non-tumor tissues (n = 83), LUAD tissues (n = 83)] and TCGA [(b) normal lung tissues (n = 287), LUAD tissues (n = 574)] datasets. DDX18 expression is indicated by the white line. (c,d) Boxplots representing DDX18 mRNA expression levels in non-tumor and LUAD tissues from GSE75037 (c) and TCGA (d) datasets. p-values are indicated. (e) Kaplan–Meier survival curve for overall survival of LUAD patients with high (n = 126) and low (n = 376) DDX18 expression. Low DDX18 expression correlates with improved overall survival (p-value indicated). (f) Relative DDX18 mRNA levels in H157 (n = 3) and H460 (n = 4) cells following DDX18 knockdown. Data represent mean ± SD. (g) Western blot analysis confirming DDX18 knockdown efficiency in H157 and H460 cells. (h) DDX18 knockdown significantly suppresses cell proliferation in H157 and H460 cells (n = 3, mean ± SD). (i) DDX18 knockdown substantially reduces colony formation capacity in H157 and H460 cells (n = 3, mean ± SD). (j) DDX18 knockdown inhibits the migration of H157 and H460 cells in vitro (n = 3, mean ± SD). ** p < 0.01; *** p < 0.001; **** p < 0.0001. LUAD, lung adenocarcinoma; SC, scramble control; KD, knockdown.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.