RECQL4 Antibody - #DF9257
(2)
| Product: | RECQL4 Antibody |
| Catalog: | DF9257 |
| Description: | Rabbit polyclonal antibody to RECQL4 |
| Application: | WB IF/ICC |
| Reactivity: | Human |
| Mol.Wt.: | 133 kDa; 133kD(Calculated). |
| Uniprot: | O94761 |
| RRID: | AB_2842453 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Clonality:
Polyclonal
Specificity:
RECQL4 Antibody detects endogenous levels of total RECQL4.
RRID:
AB_2842453
Cite Format: Affinity Biosciences Cat# DF9257, RRID:AB_2842453.
Cite Format: Affinity Biosciences Cat# DF9257, RRID:AB_2842453.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
ATP dependent DNA helicase Q4; ATP-dependent DNA helicase Q4; DNA helicase; DNA helicase, RecQ like type 4; RECQ 4; RECQ L4; RecQ protein 4; RecQ protein like 4; RecQ protein-like 4; RecQ-like type 4; RecQ4; RECQ4_HUMAN; RECQL 4; RECQL4; RTS;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- O94761 RECQ4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERLRDVRERLQAWERAFRRQRGRRPSQDDVEAAPEETRALYREYRTLKRTTGQAGGGLRSSESLPAAAEEAPEPRCWGPHLNRAATKSPQSTPGRSRQGSVPDYGQRLKANLKGTLQAGPALGRRPWPLGRASSKASTPKPPGTGPVPSFAEKVSDEPPQLPEPQPRPGRLQHLQASLSQRLGSLDPGWLQRCHSEVPDFLGAPKACRPDLGSEESQLLIPGESAVLGPGAGSQGPEASAFQEVSIRVGSPQPSSSGGEKRRWNEEPWESPAQVQQESSQAGPPSEGAGAVAVEEDPPGEPVQAQPPQPCSSPSNPRYHGLSPSSQARAGKAEGTAPLHIFPRLARHDRGNYVRLNMKQKHYVRGRALRSRLLRKQAWKQKWRKKGECFGGGGATVTTKESCFLNEQFDHWAAQCPRPASEEDTDAVGPEPLVPSPQPVPEVPSLDPTVLPLYSLGPSGQLAETPAEVFQALEQLGHQAFRPGQERAVMRILSGISTLLVLPTGAGKSLCYQLPALLYSRRSPCLTLVVSPLLSLMDDQVSGLPPCLKAACIHSGMTRKQRESVLQKIRAAQVHVLMLTPEALVGAGGLPPAAQLPPVAFACIDEAHCLSQWSHNFRPCYLRVCKVLRERMGVHCFLGLTATATRRTASDVAQHLAVAEEPDLHGPAPVPTNLHLSVSMDRDTDQALLTLLQGKRFQNLDSIIIYCNRREDTERIAALLRTCLHAAWVPGSGGRAPKTTAEAYHAGMCSRERRRVQRAFMQGQLRVVVATVAFGMGLDRPDVRAVLHLGLPPSFESYVQAVGRAGRDGQPAHCHLFLQPQGEDLRELRRHVHADSTDFLAVKRLVQRVFPACTCTCTRPPSEQEGAVGGERPVPKYPPQEAEQLSHQAAPGPRRVCMGHERALPIQLTVQALDMPEEAIETLLCYLELHPHHWLELLATTYTHCRLNCPGGPAQLQALAHRCPPLAVCLAQQLPEDPGQGSSSVEFDMVKLVDSMGWELASVRRALCQLQWDHEPRTGVRRGTGVLVEFSELAFHLRSPGDLTAEEKDQICDFLYGRVQARERQALARLRRTFQAFHSVAFPSCGPCLEQQDEERSTRLKDLLGRYFEEEEGQEPGGMEDAQGPEPGQARLQDWEDQVRCDIRQFLSLRPEEKFSSRAVARIFHGIGSPCYPAQVYGQDRRFWRKYLHLSFHALVGLATEELLQVAR
PTMs - O94761 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S27 | Phosphorylation | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| T93 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| K110 | Methylation | Uniprot | |
| K114 | Methylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| S323 | Phosphorylation | Uniprot | |
| K332 | Sumoylation | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| Y353 | Phosphorylation | Uniprot | |
| K376 | Acetylation | Uniprot | |
| K380 | Acetylation | Uniprot | |
| K382 | Acetylation | Uniprot | |
| K385 | Acetylation | Uniprot | |
| K386 | Acetylation | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| K695 | Ubiquitination | Uniprot | |
| S732 | Phosphorylation | Uniprot | |
| K738 | Ubiquitination | Uniprot | |
| T771 | Phosphorylation | Uniprot | |
| K843 | Ubiquitination | Uniprot | |
| K1048 | Ubiquitination | Uniprot | |
| K1101 | Ubiquitination | Uniprot | |
| K1154 | Ubiquitination | Uniprot |
Research Backgrounds
Function:
DNA-dependent ATPase. May modulate chromosome segregation.
Subcellular Location:
Cytoplasm. Nucleus.
Tissue Specificity:
Ubiquitously expressed, with highest levels in thymus and testis.
Subunit Structure:
Interacts with UBR1 and UBR2. Interacts with MCM10; this interaction regulates RECQL4 unwinding activity.
Family&Domains:
Belongs to the helicase family. RecQ subfamily.
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