ABCB8 Antibody | Affinity Biosciences

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Western blot analysis of ABCB8 using HeLa whole cell lysates.

Product:	ABCB8 Antibody
Catalog:	DF9249
Description:	Rabbit polyclonal antibody to ABCB8
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	80 kDa; 80kD(Calculated).
Uniprot:	Q9NUT2
RRID:	AB_2842445

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

ABCB8 Antibody detects endogenous levels of total ABCB8.

RRID:

AB_2842445
Cite Format: Affinity Biosciences Cat# DF9249, RRID:AB_2842445.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

;ATP binding cassette sub family B member 8; ABCB8; ABCB8_HUMAN; ATP binding cassette sub family B (MDR/TAP) member 8; ATP binding cassette sub family B member 8 mitochondrial; ATP-binding cassette sub-family B member 8; EST328128; M ABC1; M-ABC1; MABC1; Mitochondrial ABC protein; mitochondrial; Mitochondrial ATP binding cassette 1; Mitochondrial ATP-binding cassette 1;

Immunogens

Immunogen:

Uniprot:

Q9NUT2(MITOS_HUMAN) >>Visit HPA database.

Gene(ID):

ABCB8(11194)

Expression:

Q9NUT2 MITOS_HUMAN:

Ubiquitous.

Sequence:

Q9NUT2 MITOS_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLVHLFRVGIRGGPFPGRLLPPLRFQTFSAVRNTWRNGKTGQLHKAEGEYSDGYRSSSLLRAVAHLRSQLWAHLPRAPLAPRWSPSAWCWVGGALLGPMVLSKHPHLCLVALCEAEEAPPASSTPHVVGSRFNWKLFWQFLHPHLLVLGVAVVLALGAALVNVQIPLLLGQLVEVVAKYTRDHVGSFMTESQNLSTHLLILYGVQGLLTFGYLVLLSHVGERMAVDMRRALFSSLLRQDITFFDANKTGQLVSRLTTDVQEFKSSFKLVISQGLRSCTQVAGCLVSLSMLSTRLTLLLMVATPALMGVGTLMGSGLRKLSRQCQEQIARAMGVADEALGNVRTVRAFAMEQREEERYGAELEACRCRAEELGRGIALFQGLSNIAFNCMVLGTLFIGGSLVAGQQLTGGDLMSFLVASQTVQRSMANLSVLFGQVVRGLSAGARVFEYMALNPCIPLSGGCCVPKEQLRGSVTFQNVCFSYPCRPGFEVLKDFTLTLPPGKIVALVGQSGGGKTTVASLLERFYDPTAGVVMLDGRDLRTLDPSWLRGQVVGFISQEPVLFGTTIMENIRFGKLEASDEEVYTAAREANAHEFITSFPEGYNTVVGERGTTLSGGQKQRLAIARALIKQPTVLILDEATSALDAESERVVQEALDRASAGRTVLVIAHRLSTVRGAHCIVVMADGRVWEAGTHEELLKKGGLYAELIRRQALDAPRTAAPPPKKPEGPRSHQHKS

PTMs - Q9NUT2 As Substrate

Q9NUT2

Site	PTM Type	Source
S84	Phosphorylation	Uniprot
S86	Phosphorylation	Uniprot
S102	Phosphorylation	Uniprot
S233	Phosphorylation	Uniprot
K247	Ubiquitination	Uniprot
T257	Phosphorylation	Uniprot
K263	Ubiquitination	Uniprot
S271	Phosphorylation	Uniprot
S276	Phosphorylation	Uniprot
T295	Phosphorylation	Uniprot
T310	Phosphorylation	Uniprot
S314	Phosphorylation	Uniprot
S429	Phosphorylation	Uniprot
K465	Ubiquitination	Uniprot
K513	Ubiquitination	Uniprot
T540	Phosphorylation	Uniprot
S544	Phosphorylation	Uniprot
K573	Ubiquitination	Uniprot
S577	Phosphorylation	Uniprot
K617	Ubiquitination	Uniprot
K628	Ubiquitination	Uniprot
K699	Ubiquitination	Uniprot

Research Backgrounds

Q9NUT2_MITOS_HUMAN

Function:

ATP-binding subunit of the mitochondrial potassium channel located in the mitochondrial inner membrane. Together with CCDC51/MITOK, forms a protein complex localized in the mitochondria that mediates ATP-dependent potassium currents across the inner membrane (that is, mitoK(ATP) channel). Plays a role in mitochondrial iron transport. Required for maintenance of normal cardiac function, possibly by influencing mitochondrial iron export and regulating the maturation of cytosolic iron sulfur cluster-containing enzymes (By similarity).

Subcellular Location:

Mitochondrion inner membrane>Multi-pass membrane protein.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

The mitochondrial potassium channel (mitoK(ATP)) is composed of 4 subunits of CCDC51/MITOK and 4 subunits of ABCB8/MITOSUR (Probable). Interacts with C10orf88/PAAT. Interacts with NRP1; NRP1 regulates ABCB8/MITOSUR protein levels in mitochondria.

Family&Domains:

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Research Fields

· Environmental Information Processing > Membrane transport > ABC transporters.

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ABCB8 Antibody - #DF9249