Phospho-eIF2 alpha (Ser51)[Ser52] Antibody - AF3087

	Price	Size
	$280	100ul
	$350	200ul

Product Information

Alternative Names:Expand▼

EIF 2 alpha; EIF 2; EIF 2A; EIF 2alpha; eIF-2-alpha; eIF-2A; EIF-2alpha; EIF2 alpha; EIF2; EIF2A; EIF2S1; Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa; Eukaryotic translation initiation factor 2 subunit 1 alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; IF2A_HUMAN;

Applications:

WB 1:500-1:2000, IHC 1:50-1:500, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-eIF2 alpha (Ser51) Antibody detects endogenous levels of eIF2 alpha only when phosphorylated at Ser52, which site historically referenced as Ser51.

RRID:

AB_2834524
Please cite this product as: Affinity Biosciences Cat# AF3087, RRID:AB_2834524.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human eIF2 alpha around the phosphorylation site of Ser51.

Uniprot:

>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

EIF2S1

Molecular Weight:

Observed Mol.Wt.: 38kD.
Predicted Mol.Wt.: 36kDa(Calculated)..

Subcellular Location:

Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).

Description:

eIF2A a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex.

Sequence:

P05198 IF2A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLERENAEVDGDDDAEEMEAKAED

Research Background

P05198_IF2A_HUMAN

Function:

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Post-translational Modifications:

Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation (By similarity).

Subcellular Location:

Cytoplasm>Stress granule.
Note: Colocalizes with NANOS3 in the stress granules.

Subunit Structure:

Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interaction with METAP2 protects EIF2S1 from inhibitory phosphorylation (By similarity). Interacts with ABCF1 isoform 2. Associates with ribosomes. Interacts with DDX3X in an RNA-independent manner.

Similarity:

Belongs to the eIF-2-alpha family.

Research Fields

Research Fields:

· Cellular Processes > Transport and catabolism > Autophagy - animal.(View pathway)
· Cellular Processes > Cell growth and death > Apoptosis.(View pathway)
· Genetic Information Processing > Translation > RNA transport.
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.(View pathway)
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

ELISA analysis of AF3087 showing specificity to Phospho-eIF2 alpha (Ser51)[Ser52] Blocking Peptide peptide. Peptides concentration: 1ug/ml.
P-peptide: phospho-peptide; N-peptide: non-phospho-peptide.

Reference Citations:

1). Li X;Wang J;Gong X;Zhang M;Kang S;Shu B;Wei Z;Huang ZS;Li D; et al. Upregulation of BCL-2 by acridone derivative through gene promoter i-motif for alleviating liver damage of NAFLD/NASH. Nucleic Acids Res 2020 Sep 4;48(15):8255-8268. (PubMed: 32710621) [IF=11.501]

Application: WB Species:mouse; Sample:liver

Figure 7. Effect of A22 on ameliorating apoptosis, ER stress, inflammation, metabolic syndrome, and fibrogenesis in HF diet-fed mice. (A) Effect of A22 on BCL-2 gene transcription. (B) Effect of A22 on BAX gene transcription. (C) Effect of A22 on expressions of apoptosis-related proteins in liver. The extracted proteins from the liver were immunoblotted with specific antibodies, and quantified based on the loading control of ACTIN. (D) Effect of A22 on ER stress. The UPR proteins (IRE-1, PERK, elF-2 and CHOP) were analyzed by using western Blot. (E) Effect of A22 on expressions of inflammatory factors. (F) Effect of A22 on expressions of fibrogenic proteins.

2). Cai Y et al. Betulinic acid chemosensitizes breast cancer by triggering ER stress-mediated apoptosis by directly targeting GRP78. Cell Death Dis 2018 May 25;9(6):636 (PubMed: 29802332) [IF=6.304]

Application: WB Species:human; Sample:MCF-7,MDA-MB-231

Fig. 5 BA triggers breast cancer cells apoptosis via ER stress-mediated pathway. a MCF-7 and MDA-MB-231 cells were treated with the indicated concentrations of BA for 24 h, and the protein levels of ER stress-associated signals were stimulated by BA in a dose-dependent manner, including GRP78, p-PERK/PERK, p-eIF2α/eIF2α, CHOP, and caspase-12. b MCF-7 and MDA-MB-231 cells were treated with BA alone or in combination with taxol for 24 h, the expression levels of GRP78, p-PERK/PERK, p-eIF2α/eIF2α, CHOP, and caspase-12 were also significantly upregulated following drug administration, especially in the co-treatment group, indicating the ER stress-mediated apoptosis pathway was aggravatedly activated by drug combination.

3). Zhao YS et al. Hydrogen and Oxygen Mixture to Improve Cardiac Dysfunction and Myocardial Pathological Changes Induced by Intermittent Hypoxia in Rats. Oxid Med Cell Longev 2019 Mar 7;2019:7415212 (PubMed: 30984338) [IF=5.076]

4). Zheng Y;Zhou Q;Zhao C;Li J;Yu Z;Zhu Q; et al. ATP citrate lyase inhibitor triggers endoplasmic reticulum stress to induce hepatocellular carcinoma cell apoptosis via p‐eIF2α/ATF4/CHOP axis. J Cell Mol Med 2021 Feb;25(3):1468-1479. (PubMed: 33393219) [IF=4.658]

5). Zheng Y;Zhou Q;Zhao C;Li J;Yu Z;Zhu Q; et al. ATP citrate lyase inhibitor triggers endoplasmic reticulum stress to induce hepatocellular carcinoma cell apoptosis via p‐eIF2α/ATF4/CHOP axis. J Cell Mol Med 2021 Feb;25(3):1468-1479. (PubMed: 33393219) [IF=4.486]

6). Liu Y et al. EndophilinA2 protects against angiotensin II-induced cardiac hypertrophy by inhibiting angiotensin II type 1 receptor trafficking in neonatal rat cardiomyocytes. J Cell Biochem 2018 Jun 20 (PubMed: 29923351) [IF=4.237]

7). Wu X et al. Protective Effect of Patchouli Alcohol Against High-Fat Diet Induced Hepatic Steatosis by Alleviating Endoplasmic Reticulum Stress and Regulating VLDL Metabolism in Rats. Front Pharmacol 2019 Oct 1;10:1134 (PubMed: 31632274) [IF=4.225]

8). Li F et al. Icariin improves the cognitive function of APP/PS1 mice via suppressing endoplasmic reticulum stress. Life Sci 2019 Aug 7:116739 (PubMed: 31400352) [IF=3.647]

9). Song Z et al. The protective role of the MKP-5-JNK/P38 pathway in glucolipotoxicity-induced islet β-cell dysfunction and apoptosis. Exp Cell Res 2019 Jun 13 (PubMed: 31202710) [IF=3.383]

10). Qiuli H et al. [EXPRESS] Endoplasmic reticulum stress promoting caspase signaling pathway dependent apoptosis contributes to bone cancer pain in the spinal dorsal horn. Mol Pain 2019 Aug 27:1744806919876150 (PubMed: 31452457)

11). Wu K et al. Antitumor effect of ginsenoside Rg3 on gallbladder cancer by inducing endoplasmic reticulum stress-mediated apoptosis in vitro and in vivo. Oncol Lett 2018 Nov;16(5):5687-5696 (PubMed: 30344724)

12). Zhang J et al. Inhibition of the SIRT1 signaling pathway exacerbates endoplasmic reticulum stress induced by renal ischemia/reperfusion injury in type 1 diabetic rats. Mol Med Rep 2020 Feb;21(2):695-704 (PubMed: 31974604)

13). Wu Y;Cui H;Zhang Y;Yu P;Li Y;Wu D;Xue Y;Fu W; et al. Inonotus obliquus extract alleviates myocardial ischemia/reperfusion injury by suppressing endoplasmic reticulum stress. Mol Med Rep 2021 Jan;23(1):77. (PubMed: 33236154)

14). Yuan S;Liang X;He W;Liang M;Jin J;He Q; et al. ATF4-dependent heme-oxygenase-1 attenuates diabetic nephropathy by inducing autophagy and inhibiting apoptosis in podocyte. Ren Fail 2021 Dec;43(1):968-979. (PubMed: 34157937)

15). et al. The Pathology of Morphine-Inhibited Nerve Repair and Morphine-Induced Nerve Damage Is Mediated via Endoplasmic Reticulum Stress.

16). et al. The novel curcumin derivative 1g induces mitochondrial and ER-stress-dependent apoptosis in colon cancer cells by induction of ROS production.

17). Li W;Xu X;Dong D;Lei T;Ou H; et al. Up-regulation of thioredoxin system by puerarin inhibits lipid uptake in macrophages. Free Radic Biol Med 2020 Nov 24;S0891-5849(20)31609-9. (PubMed: 33242606)

18). Yuan Q;Xu T;Chen Y;Qu W;Sun D;Liu X;Sun L; et al. MiR-185-5p ameliorates endoplasmic reticulum stress and renal fibrosis by downregulation of ATF6. Lab Invest 2020 Jun 8. (PubMed: 32514126)

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Catalog Number :

AF3087-BP
(Blocking peptide available as AF3087-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig

100%

Horse

100%

Bovine

100%

Sheep

100%

Dog

100%

Xenopus

100%

Chicken

100%

Rabbit

100%

Zebrafish

High similarity Medium similarity Low similarity No similarity

P05198 as Substrate

P05198

Site	PTM Type	Enzyme	Source
S5	Phosphorylation		Uniprot
K12	Ubiquitination		Uniprot
S26	Phosphorylation		Uniprot
S49	Phosphorylation	Q9NZJ5 (EIF2AK3) , Q9BQI3 (EIF2AK1) , P19525 (EIF2AK2) , Q75MR0 (HRI)	Uniprot
S52	Phosphorylation	B4DS64 (PRKRIR) , P51812 (RPS6KA3) , P19525 (EIF2AK2) , P28482 (MAPK1) , Q75MR0 (HRI) , Q9BQI3 (EIF2AK1) , Q9NZJ5 (EIF2AK3) , Q05655 (PRKCD) , Q16539 (MAPK14) , Q9P2K8 (EIF2AK4)	Uniprot
S58	Phosphorylation		Uniprot
K61	Ubiquitination		Uniprot
K80	Ubiquitination		Uniprot
Y82	Phosphorylation		Uniprot
S86	Phosphorylation		Uniprot
K87	Ubiquitination		Uniprot
S91	Phosphorylation		Uniprot
K97	Ubiquitination		Uniprot
K101	Acetylation		Uniprot
K101	Ubiquitination		Uniprot
K104	Ubiquitination		Uniprot
K106	Ubiquitination		Uniprot
S110	Phosphorylation		Uniprot
K123	Ubiquitination		Uniprot
R133	Methylation		Uniprot
K141	Acetylation		Uniprot
K141	Ubiquitination		Uniprot
K143	Acetylation		Uniprot
K143	Ubiquitination		Uniprot
Y147	Phosphorylation		Uniprot
Y150	Phosphorylation		Uniprot
K154	Methylation		Uniprot
K154	Ubiquitination		Uniprot
S158	Phosphorylation		Uniprot
T185	Phosphorylation		Uniprot
K190	Ubiquitination		Uniprot
Y200	Phosphorylation		Uniprot
Y202	Phosphorylation		Uniprot
K209	Ubiquitination		Uniprot
S219	Phosphorylation		Uniprot
K226	Ubiquitination		Uniprot
Y235	Phosphorylation		Uniprot
T245	Phosphorylation		Uniprot
K259	Ubiquitination		Uniprot
K265	Ubiquitination		Uniprot
R266	Methylation		Uniprot
K276	Ubiquitination		Uniprot
T279	Phosphorylation		Uniprot
T281	Phosphorylation		Uniprot
T284	Phosphorylation		Uniprot
K312	Ubiquitination		Uniprot

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

Secondary antibody Goat Anti-Rabbit IgG (H+L) HRP (S0001) Goat Anti-Rabbit IgG (H+L) AP (S0003) Goat Anti-Rabbit IgG (H+L) Fluor594-conjugated (S0006) Goat Anti-Rabbit IgG (H+L) FITC-conjugated (S0008) Goat Anti-Rabbit IgG (H+L) CY3-conjugated (S0011) Goat Anti-Rabbit IgG (H+L) Fluor647-conjugated (S0013) Goat Anti-Rabbit IgG (H+L) TRITC-conjugated (S0015) Goat Anti-Rabbit IgG (H+L) Fluor488-conjugated (S0018)

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