PDS5B Antibody - #DF9206

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Product: PDS5B Antibody
Catalog: DF9206
Description: Rabbit polyclonal antibody to PDS5B
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 165 kDa; 165kD(Calculated).
Uniprot: Q9NTI5
RRID: AB_2842402

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PDS5B Antibody detects endogenous levels of total PDS5B.
RRID:
AB_2842402
Cite Format: Affinity Biosciences Cat# DF9206, RRID:AB_2842402.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI646570; Androgen induced proliferation inhibitor; Androgen-induced prostate proliferative shutoff associated protein AS3; APRIN; AS3; AW212954; CG008; FLJ23236; KIAA0979; mKIAA0979; PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae); Pds5b; RP1 267P19.1; Sister chromatid cohesion protein PDS5 homolog B;

Immunogens

Immunogen:
Uniprot:

Q9NTI5(PDS5B_HUMAN) >>Visit HPA database.

Gene(ID):
PDS5B(23047)
Expression:
Q9NTI5 PDS5B_HUMAN:

Widely expressed.

Sequence:
MAHSKTRTNDGKITYPPGVKEISDKISKEEMVRRLKMVVKTFMDMDQDSEEEKELYLNLALHLASDFFLKHPDKDVRLLVACCLADIFRIYAPEAPYTSPDKLKDIFMFITRQLKGLEDTKSPQFNRYFYLLENIAWVKSYNICFELEDSNEIFTQLYRTLFSVINNGHNQKVHMHMVDLMSSIICEGDTVSQELLDTVLVNLVPAHKNLNKQAYDLAKALLKRTAQAIEPYITNFFNQVLMLGKTSISDLSEHVFDLILELYNIDSHLLLSVLPQLEFKLKSNDNEERLQVVKLLAKMFGAKDSELASQNKPLWQCYLGRFNDIHVPIRLECVKFASHCLMNHPDLAKDLTEYLKVRSHDPEEAIRHDVIVSIVTAAKKDILLVNDHLLNFVRERTLDKRWRVRKEAMMGLAQIYKKYALQSAAGKDAAKQIAWIKDKLLHIYYQNSIDDRLLVERIFAQYMVPHNLETTERMKCLYYLYATLDLNAVKALNEMWKCQNLLRHQVKDLLDLIKQPKTDASVKAIFSKVMVITRNLPDPGKAQDFMKKFTQVLEDDEKIRKQLEVLVSPTCSCKQAEGCVREITKKLGNPKQPTNPFLEMIKFLLERIAPVHIDTESISALIKQVNKSIDGTADDEDEGVPTDQAIRAGLELLKVLSFTHPISFHSAETFESLLACLKMDDEKVAEAALQIFKNTGSKIEEDFPHIRSALLPVLHHKSKKGPPRQAKYAIHCIHAIFSSKETQFAQIFEPLHKSLDPSNLEHLITPLVTIGHIALLAPDQFAAPLKSLVATFIVKDLLMNDRLPGKKTTKLWVPDEEVSPETMVKIQAIKMMVRWLLGMKNNHSKSGTSTLRLLTTILHSDGDLTEQGKISKPDMSRLRLAAGSAIVKLAQEPCYHEIITLEQYQLCALAINDECYQVRQVFAQKLHKGLSRLRLPLEYMAICALCAKDPVKERRAHARQCLVKNINVRREYLKQHAAVSEKLLSLLPEYVVPYTIHLLAHDPDYVKVQDIEQLKDVKECLWFVLEILMAKNENNSHAFIRKMVENIKQTKDAQGPDDAKMNEKLYTVCDVAMNIIMSKSTTYSLESPKDPVLPARFFTQPDKNFSNTKNYLPPEMKSFFTPGKPKTTNVLGAVNKPLSSAGKQSQTKSSRMETVSNASSSSNPSSPGRIKGRLDSSEMDHSENEDYTMSSPLPGKKSDKRDDSDLVRSELEKPRGRKKTPVTEQEEKLGMDDLTKLVQEQKPKGSQRSRKRGHTASESDEQQWPEEKRLKEDILENEDEQNSPPKKGKRGRPPKPLGGGTPKEEPTMKTSKKGSKKKSGPPAPEEEEEEERQSGNTEQKSKSKQHRVSRRAQQRAESPESSAIESTQSTPQKGRGRPSKTPSPSQPKKNVRVGRSKQAATKENDSSEEVDVFQGSSPVDDIPQEETEEEEVSTVNVRRRSAKRERR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
80
Pig
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NTI5 As Substrate

Site PTM Type Enzyme
K12 Acetylation
K20 Acetylation
K20 Ubiquitination
K28 Ubiquitination
T41 Phosphorylation
S49 Phosphorylation
K53 Acetylation
T98 Phosphorylation
T111 Phosphorylation
K121 Ubiquitination
K212 Acetylation
Y215 Phosphorylation
K219 Acetylation
S283 Phosphorylation
K294 Ubiquitination
K312 Ubiquitination
K349 Ubiquitination
Y354 Phosphorylation
K356 Acetylation
K356 Ubiquitination
K380 Ubiquitination
K418 Ubiquitination
Y419 Phosphorylation
K427 Ubiquitination
K507 Ubiquitination
K514 Ubiquitination
T518 Phosphorylation
T533 Phosphorylation
K558 Ubiquitination
K574 Ubiquitination
K627 Ubiquitination
S628 Phosphorylation
K693 Ubiquitination
K698 Ubiquitination
K717 Ubiquitination
Y728 Phosphorylation
S787 Phosphorylation
R802 Methylation
K810 Ubiquitination
S844 Phosphorylation
K872 Ubiquitination
K974 Ubiquitination
S985 Phosphorylation
Y990 Phosphorylation
Y1005 Phosphorylation
K1015 Ubiquitination
T1050 Phosphorylation
S1087 Phosphorylation
K1089 Ubiquitination
K1103 Acetylation
K1103 Ubiquitination
K1109 Ubiquitination
S1118 Phosphorylation
T1121 Phosphorylation
K1136 Acetylation
S1139 Phosphorylation
S1140 Phosphorylation
S1145 Phosphorylation
T1147 Phosphorylation
T1154 Phosphorylation
S1156 Phosphorylation
S1159 Phosphorylation
S1160 Phosphorylation
S1161 Phosphorylation
S1162 Phosphorylation
S1165 Phosphorylation
S1166 Phosphorylation
S1176 Phosphorylation
S1177 Phosphorylation
S1182 Phosphorylation
Y1187 Phosphorylation
T1188 Phosphorylation
S1190 Phosphorylation
S1191 Phosphorylation
S1204 Phosphorylation
S1209 Phosphorylation
K1213 Acetylation
T1220 Phosphorylation
K1228 Acetylation
T1235 Phosphorylation
S1246 Phosphorylation
T1255 Phosphorylation
S1257 Phosphorylation
S1259 Phosphorylation
S1283 Phosphorylation
T1301 Phosphorylation
S1319 Phosphorylation
S1334 Phosphorylation
K1344 Methylation
S1349 Phosphorylation
S1358 Phosphorylation
S1361 Phosphorylation
S1362 Phosphorylation
S1366 Phosphorylation
T1367 Phosphorylation
S1369 Phosphorylation P24941 (CDK2)
T1370 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
S1379 Phosphorylation
T1381 Phosphorylation
S1383 Phosphorylation
S1385 Phosphorylation
S1407 Phosphorylation
S1416 Phosphorylation
S1417 Phosphorylation
S1441 Phosphorylation

Research Backgrounds

Function:

Regulator of sister chromatid cohesion in mitosis which may stabilize cohesin complex association with chromatin. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Plays a role in androgen-induced proliferative arrest in prostate cells.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Interacts with the cohesin complex. Interacts with RAD21; the interaction is direct. Interacts with WAPL (via FGF motifs) or CDCA5 (via the FGF motif); the interaction is direct, cohesin-dependent and competitive (Probable).

Family&Domains:

Belongs to the PDS5 family.

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