ACCN2 Antibody - #DF9198

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Product: ACCN2 Antibody
Catalog: DF9198
Description: Rabbit polyclonal antibody to ACCN2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 60 kDa; 60kD(Calculated).
Uniprot: P78348
RRID: AB_2842394

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
ACCN2 Antibody detects endogenous levels of total ACCN2.
RRID:
AB_2842394
Cite Format: Affinity Biosciences Cat# DF9198, RRID:AB_2842394.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACCN2; acid-sensing (proton-gated) ion channel 1; acid-sensing ion channel 1; acid-sensing ion channel 1a protein; amiloride-sensitive cation channel 2, neuronal; ASIC; BNaC2; brain sodium channel 2; Cation channel, amiloride-sensitive, neuronal, 2;

Immunogens

Immunogen:
Uniprot:

P78348(ASIC1_HUMAN) >>Visit HPA database.

Gene(ID):
ASIC1(41)
Expression:
P78348 ASIC1_HUMAN:

Expressed in most or all neurons.

Sequence:
MELKAEEEEVGGVQPVSIQAFASSSTLHGLAHIFSYERLSLKRALWALCFLGSLAVLLCVCTERVQYYFHYHHVTKLDEVAASQLTFPAVTLCNLNEFRFSQVSKNDLYHAGELLALLNNRYEIPDTQMADEKQLEILQDKANFRSFKPKPFNMREFYDRAGHDIRDMLLSCHFRGEVCSAEDFKVVFTRYGKCYTFNSGRDGRPRLKTMKGGTGNGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVACQEQRLIYLPPPWGTCKAVTMDSDLDFFDSYSITACRIDCETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDFLVEKDQEYCVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKFNKSEQYIGENILVLDIFFEVLNYETIEQKKAYEIAGLLGDIGGQMGLFIGASILTVLELFDYAYEVIKHKLCRRGKCQKEAKRSSADKGVALSLDDVKRHNPCESLRGHPAGMTYAANILPHHPARGTFEDFTC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78348 As Substrate

Site PTM Type Enzyme
S25 Phosphorylation
S40 Phosphorylation
K193 Ubiquitination
T316 Phosphorylation
K374 Ubiquitination
S479 Phosphorylation P17612 (PRKACA)
K492 Ubiquitination
S499 Phosphorylation

Research Backgrounds

Function:

Isoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.

Isoform 1 does not display proton-gated cation channel activity.

PTMs:

Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4 (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in most or all neurons.

Subunit Structure:

Homotrimer or heterotrimer with other ASIC proteins (By similarity). Interacts with STOM and ASIC2 (By similarity). Interacts with PRKCABP. Homotrimer of Asic1a (ASIC1 isoform 2) interacts with spider venom psalmotoxin-1. Homotrimer of Asic1a (ASIC1 isoform 2) and Asic1b (ASIC1 isoform 3) interacts with the spider venom pi-theraphotoxin-Hm3a. Homotrimer of Asic1a (ASIC1 isoform 2) interacts with the snake venom mambalgin-1. Heterotrimer of Asic1a-Asic2a (ASIC1 isoform 2-ASIC2 isoform 1) interacts with the snake venom mambalgin-1.

Family&Domains:

Channel opening involves a conformation change that affects primarily the extracellular domain and the second transmembrane helix and its orientation in the membrane. In the open state, the second transmembrane helix is nearly perpendicular to the plane of the membrane; in the desensitized state it is strongly tilted. Besides, the second transmembrane domain is discontinuously helical in the open state. The GAS motif of the selectivity filter is in an extended conformation, giving rise to a distinct kink in the polypeptide chain. A domain swap between subunits gives rise to a full-length transmembrane helix (By similarity).

Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC1 subfamily.

Research Fields

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

References

1). Nerve growth factor promotes ASIC1a expression via the NF-κB pathway and enhances acid-induced chondrocyte apoptosis. International Immunopharmacology (PubMed: 32146316) [IF=5.6]

Application: WB    Species: rat    Sample: articular chondrocytes

Fig. 1. |NGF up-regulated ASIC1a expression in rat articular chondrocytes. (A) Extracorporeal culture and identification of primary articular chondrocytes in rats. (i) The biosynthesis of intracellular glycosaminoglycan stained positive with toluidine blue. (ii) Immunohistochemical staining of collagen. (B and D) NGF stimulated the expression of ASIC1a mRNA and protein in a concentration-dependent manner. Treating the cells with or without the indicated NGF concentrations (5–40 ng/ml) for 24 h.
2). Acidosis induces synovial fibroblasts to release vascular endothelial growth factor via acid-sensitive ion channel 1a. LABORATORY INVESTIGATION (PubMed: 32826932) [IF=5.0]

Application: WB    Species: human    Sample: synovial

Fig. 1| ASIC1a is highly expressed in RA synovial tissue. a Immunohistochemical visualization of ASIC1a in normal human synovial tissue and synovial tissue in RA patients (normal: n = 3, RA: n = 25)***P < 0.001 versus normal group. b, c Detection of CD55 and ASIC1a in RASF by flow cytometry. d Western blotting analysis of ASIC1a and 2,3,4 protein expression in RASF.Histograms show semiquantitative analysis of gels from western blotting.
3). Nesfatin-1 exerts protective effects on acidosis-stimulated chondrocytes and rats with adjuvant-induced arthritis by inhibiting ASIC1a expression. Laboratory Investigation (PubMed: 35292759) [IF=5.0]
4). 17β-estradiol attenuates rat articular chondrocyte injury by targeting ASIC1a-mediated apoptosis. MOLECULAR AND CELLULAR ENDOCRINOLOGY (PubMed: 32006608) [IF=4.1]

Application: WB    Species: rat    Sample: chondrocyte

Fig. 2. |Effects of 17β-E2 on ASIC1a expression and activity. A. Western blot analysis of ASIC1a protein in chondrocyte in different concentrations. C. Western blot analysis of ASIC1a protein in different times.

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