Product: Phospho-JIP1 (Thr103) Antibody
Catalog: DF8740
Source: Rabbit
Application: WB, IF/ICC, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine
Mol.Wt.: 77 kD; 78kD(Calculated).
Uniprot: Q9UQF2
RRID: AB_2841944

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%)
Clonality:
Polyclonal
Specificity:
Phospho-JIP1 (Thr103) Antibody detects endogenous levels of JIP1 only when phosphorylated at Thr103.
RRID:
AB_2841944
Cite Format: Affinity Biosciences Cat# DF8740, RRID:AB_2841944.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C jun amino terminal kinase interacting protein 1; C-jun-amino-terminal kinase-interacting protein 1; IB 1; IB-1; IB1; Islet brain 1; Islet-brain 1; JIP 1; JIP-1; JIP1_HUMAN; JNK interacting protein 1; JNK MAP kinase scaffold protein 1; JNK-interacting protein 1; MAPK8IP 1; MAPK8IP1; MAPK8IP1 mitogen-activated protein kinase 8 interacting protein 1; Mitogen activated protein kinase 8 interacting protein 1; Mitogen-activated protein kinase 8-interacting protein 1; PRKM8; PRKM8 interacting protein; PRKM8IP;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9UQF2 JIP1_HUMAN:

Highly expressed in brain. Expressed in neurons, localizing to neurite tips in differentiating cells. Also expressed in the pancreas, testis and prostate. Low levels in heart, ovary and small intestine. Decreased levels in pancreatic beta cells sensitize cells to IL-1-beta-induced apoptosis.

Sequence:
MAERESGGLGGGAASPPAASPFLGLHIASPPNFRLTHDISLEEFEDEDLSEITDECGISLQCKDTLSLRPPRAGLLSAGGGGAGSRLQAEMLQMDLIDATGDTPGAEDDEEDDDEERAARRPGAGPPKAESGQEPASRGQGQSQGQSQGPGSGDTYRPKRPTTLNLFPQVPRSQDTLNNNSLGKKHSWQDRVSRSSSPLKTGEQTPPHEHICLSDELPPQSGPAPTTDRGTSTDSPCRRSTATQMAPPGGPPAAPPGGRGHSHRDRIHYQADVRLEATEEIYLTPVQRPPDAAEPTSAFLPPTESRMSVSSDPDPAAYPSTAGRPHPSISEEEEGFDCLSSPERAEPPGGGWRGSLGEPPPPPRASLSSDTSALSYDSVKYTLVVDEHAQLELVSLRPCFGDYSDESDSATVYDNCASVSSPYESAIGEEYEEAPRPQPPACLSEDSTPDEPDVHFSKKFLNVFMSGRSRSSSAESFGLFSCIINGEEQEQTHRAIFRFVPRHEDELELEVDDPLLVELQAEDYWYEAYNMRTGARGVFPAYYAIEVTKEPEHMAALAKNSDWVDQFRVKFLGSVQVPYHKGNDVLCAAMQKIATTRRLTVHFNPPSSCVLEISVRGVKIGVKADDSQEAKGNKCSHFFQLKNISFCGYHPKNNKYFGFITKHPADHRFACHVFVSEDSTKALAESVGRAFQQFYKQFVEYTCPTEDIYLE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Horse
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UQF2 As Substrate

Site PTM Type Enzyme
S15 Phosphorylation P45983 (MAPK8)
S29 Phosphorylation P45983 (MAPK8)
S40 Phosphorylation
R86 Methylation
T100 Phosphorylation
T103 Phosphorylation P45984 (MAPK9) , P45983 (MAPK8)
K128 Acetylation
S152 Phosphorylation
S181 Phosphorylation
S187 Phosphorylation
S193 Phosphorylation
S195 Phosphorylation
S196 Phosphorylation
S197 Phosphorylation P45983 (MAPK8)
T201 Phosphorylation
T205 Phosphorylation P45983 (MAPK8)
S214 Phosphorylation
S235 Phosphorylation
S240 Phosphorylation
T241 Phosphorylation
T243 Phosphorylation
T284 Phosphorylation P45983 (MAPK8)
S311 Phosphorylation
S328 Phosphorylation
S330 Phosphorylation
S340 Phosphorylation
S341 Phosphorylation P45983 (MAPK8)
S355 Phosphorylation
S366 Phosphorylation
S369 Phosphorylation
S407 Phosphorylation
S409 Phosphorylation
T411 Phosphorylation
S421 Phosphorylation P45983 (MAPK8)
S444 Phosphorylation
S447 Phosphorylation
T448 Phosphorylation
S469 Phosphorylation
S471 Phosphorylation
S472 Phosphorylation
S473 Phosphorylation
S574 Phosphorylation
T702 Phosphorylation

Research Backgrounds

Function:

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and inhibiting JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response. Acts as a scaffold protein that coordinates with SH3RF1 in organizing different components of the JNK pathway, including RAC1 or RAC2, MAP3K11/MLK3 or MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and/or MAPK9/JNK2 into a functional multiprotein complex to ensure the effective activation of the JNK signaling pathway. Regulates the activation of MAPK8/JNK1 and differentiation of CD8(+) T-cells.

PTMs:

Phosphorylated by MAPK8, MAPK9 and MAPK10. Phosphorylation on Thr-103 is also necessary for the dissociation and activation of MAP3K12. Phosphorylated by isoform 1 and isoform 2 of VRK2. Hyperphosphorylated during mitosis following activation of stress-activated and MAP kinases.

Ubiquitinated. Two preliminary events are required to prime for ubiquitination; phosphorylation and an increased in intracellular calcium concentration. Then, the calcium influx initiates ubiquitination and degradation by the ubiquitin-proteasome pathway.

Subcellular Location:

Cytoplasm. Cytoplasm>Perinuclear region. Nucleus. Endoplasmic reticulum membrane. Mitochondrion membrane.
Note: Accumulates in cell surface projections. Under certain stress conditions, translocates to the perinuclear region of neurons. In insulin-secreting cells, detected in both the cytoplasm and nucleus (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in brain. Expressed in neurons, localizing to neurite tips in differentiating cells. Also expressed in the pancreas, testis and prostate. Low levels in heart, ovary and small intestine. Decreased levels in pancreatic beta cells sensitize cells to IL-1-beta-induced apoptosis.

Subunit Structure:

Forms homo- or heterooligomeric complexes. Binds specific components of the JNK signaling pathway namely, MAPK8/JNK1, MAPK9/JNK2, MAPK10/JNK3, MAP2K7/MKK7, MAP3K11/MLK3 and DLK1. Also binds the proline-rich domain-containing splice variant of apolipoprotein E receptor 2 (ApoER2). Interacts, via the PID domain, with ARHGEF28. Binds the cytoplasmic tails of LRP1 and LRP2 (Megalin). Binds the TPR motif-containing C-terminal of KNS2, then the pre-assembled MAPK8IP1 scaffolding complexes are transported as a cargo of kinesin, to the required subcellular location. Interacts with the cytoplasmic domain of APP. Interacts with DCLK2 (By similarity). Interacts with MAP3K7/TAK1. Interacts with isoform 1 and isoform 2 of VRK2. Found in a complex with SH3RF1, RAC1, MAP3K11/MLK3, MAP2K7/MKK7 and MAPK8/JNK1. Found in a complex with SH3RF1, RAC2, MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and MAPK9/JNK2. Interacts with SH3RF2 (By similarity).

Family&Domains:

The destruction boxes (D-box) may act as recognition signals for degradation via the ubiquitin-proteasome pathway.

A minimal inhibitory domain prevents pancreatic beta cell apoptosis in vitro, and prevents activation of c-jun by MAPK8, MAPK9 and MAPK10.

The SH3 domain mediates homodimerization.

Belongs to the JIP scaffold family.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

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