IL4R/CD124 Antibody | Affinity Biosciences

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Product:	IL4R/CD124 Antibody
Catalog:	DF8567
Description:	Rabbit polyclonal antibody to IL4R/CD124
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Horse, Dog
Mol.Wt.:	89 kDa; 90kD(Calculated).
Uniprot:	P24394
RRID:	AB_2841771

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Horse(100%), Dog(88%)

Clonality:

Polyclonal

Specificity:

IL4R/CD124 Antibody detects endogenous levels of total IL4R/CD124.

RRID:

AB_2841771
Cite Format: Affinity Biosciences Cat# DF8567, RRID:AB_2841771.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD124; IL 4R alpha; IL-4 receptor subunit alpha; IL-4-binding protein; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; IL4-BP; Il4r; IL4R nirs variant 1; IL4RA; IL4RA_HUMAN; Interleukin 4 receptor alpha chain; Interleukin 4 receptor; sIL4Ralpha/prot; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; Soluble interleukin-4 receptor subunit alpha;

Immunogens

Immunogen:

Uniprot:

P24394(IL4RA_HUMAN) >>Visit HPA database.

Gene(ID):

IL4R(3566)

Expression:

P24394 IL4RA_HUMAN:

Isoform 1 and isoform 2 are highly expressed in activated T-cells.

Sequence:

P24394 IL4RA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGWLCSGLLFPVSCLVLLQVASSGNMKVLQEPTCVSDYMSISTCEWKMNGPTNCSTELRLLYQLVFLLSEAHTCIPENNGGAGCVCHLLMDDVVSADNYTLDLWAGQQLLWKGSFKPSEHVKPRAPGNLTVHTNVSDTLLLTWSNPYPPDNYLYNHLTYAVNIWSENDPADFRIYNVTYLEPSLRIAASTLKSGISYRARVRAWAQCYNTTWSEWSPSTKWHNSYREPFEQHLLLGVSVSCIVILAVCLLCYVSITKIKKEWWDQIPNPARSRLVAIIIQDAQGSQWEKRSRGQEPAKCPHWKNCLTKLLPCFLEHNMKRDEDPHKAAKEMPFQGSGKSAWCPVEISKTVLWPESISVVRCVELFEAPVECEEEEEVEEEKGSFCASPESSRDDFQEGREGIVARLTESLFLDLLGEENGGFCQQDMGESCLLPPSGSTSAHMPWDEFPSAGPKEAPPWGKEQPLHLEPSPPASPTQSPDNLTCTETPLVIAGNPAYRSFSNSLSQSPCPRELGPDPLLARHLEEVEPEMPCVPQLSEPTTVPQPEPETWEQILRRNVLQHGAAAAPVSAPTSGYQEFVHAVEQGGTQASAVVGLGPPGEAGYKAFSSLLASSAVSPEKCGFGASSGEEGYKPFQDLIPGCPGDPAPVPVPLFTFGLDREPPRSPQSSHLPSSSPEHLGLEPGEKVEDMPKPPLPQEQATDPLVDSLGSGIVYSALTCHLCGHLKQCHGQEDGGQTPVMASPCCGCCCGDRSSPPTTPLRAPDPSPGGVPLEASLCPASLAPSGISEKSKSSSSFHPAPGNAQSSSQTPKIVNFVSVGPTYMRVS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

Rabbit

Pig

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P24394 As Substrate

P24394

Site	PTM Type	Source
K192	Ubiquitination	Uniprot
S383	Phosphorylation	Uniprot
S387	Phosphorylation	Uniprot
S390	Phosphorylation	Uniprot
S391	Phosphorylation	Uniprot
S470	Phosphorylation	Uniprot
S474	Phosphorylation	Uniprot
T476	Phosphorylation	Uniprot
Y497	Phosphorylation	Uniprot
Y575	Phosphorylation	Uniprot
Y603	Phosphorylation	Uniprot
S616	Phosphorylation	Uniprot
Y631	Phosphorylation	Uniprot
Y713	Phosphorylation	Uniprot
S753	Phosphorylation	Uniprot
T756	Phosphorylation	Uniprot
S791	Phosphorylation	Uniprot
T820	Phosphorylation	Uniprot
Y821	Phosphorylation	Uniprot

Research Backgrounds

P24394_IL4RA_HUMAN

Function:

Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.

Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.

PTMs:

On IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.

The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Secreted.

Tissue Specificity:

Isoform 1 and isoform 2 are highly expressed in activated T-cells.

Subunit Structure:

The functional IL4 receptor is formed by initial binding of IL4 to IL4R. Subsequent recruitment to the complex of the common gamma chain, in immune cells, creates a type I receptor and, in non-immune cells, of IL13RA1 forms a type II receptor. IL4R can also interact with the IL13/IL13RA1 complex to form a similar type II receptor. Interacts with PIK3C3 (By similarity). Interacts with the SH2-containing phosphatases, PTPN6/SHIP1, PTPN11/SHIP2 and INPP5D/SHIP. Interacts with JAK1 through a Box 1-containing region; inhibited by SOCS5. Interacts with SOCS5; inhibits IL4 signaling (By similarity). Interacts with JAK3. Interacts with CLM1 (By similarity).

Family&Domains:

The extracellular domain represents the IL4 binding protein (IL4BP).

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

Belongs to the type I cytokine receptor family. Type 4 subfamily.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)

References

1). Constructing a better binding peptide for drug delivery targeting the interleukin-4 receptor. JOURNAL OF DRUG TARGETING (PubMed: 32363946) [IF=4.5]

Application: WB Species: human Sample: tumor cell

Figure 3.| ILBP-b is a better IL-4R-targeted peptide than ILBP-a. (A) Expression of IL-4Rα in different tumor cell lines. GAPDH was used as an internal control. And gray value analysis was performed on each lane in the Western Blot results map using ImageJ software. The obtained values were used as the numerical results of IL-4Rα relative expression levels in the manner of IL-4Rα/GAPDH. The error bars represent the mean ± SD, N=3.**p<0.01, ***p <0.001, compared with SMMC group.

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IL4R/CD124 Antibody - #DF8567