Product: CD226/DNAM-1 Antibody
Catalog: AF0087
Description: Rabbit polyclonal antibody to CD226/DNAM-1
Application: WB IF/ICC
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Horse
Mol.Wt.: 38kDa; 39kD(Calculated).
Uniprot: Q15762
RRID: AB_2833272

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(88%), Horse(100%)
Clonality:
Polyclonal
Specificity:
CD226/DNAM-1 Antibody detects endogenous levels of total CD226/DNAM-1.
RRID:
AB_2833272
Cite Format: Affinity Biosciences Cat# AF0087, RRID:AB_2833272.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

adhesion glycoprotein; CD226; CD226 antigen; CD226 molecule; CD226_HUMAN; DNAM 1; DNAM-1; DNAM1; DNAX accessory molecule 1; platelet and T cell activation antigen 1; PTA1; T lineage specific activation antigen 1 antigen; TLiSA1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q15762 CD226_HUMAN:

Expressed by peripheral blood T-lymphocytes.

Description:
DNAM-1 Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell. Interacts with PVR and PVRL2.
Sequence:
MDYPTLLLALLHVYRALCEEVLWHTSVPFAENMSLECVYPSMGILTQVEWFKIGTQQDSIAIFSPTHGMVIRKPYAERVYFLNSTMASNNMTLFFRNASEDDVGYYSCSLYTYPQGTWQKVIQVVQSDSFEAAVPSNSHIVSEPGKNVTLTCQPQMTWPVQAVRWEKIQPRQIDLLTYCNLVHGRNFTSKFPRQIVSNCSHGRWSVIVIPDVTVSDSGLYRCYLQASAGENETFVMRLTVAEGKTDNQYTLFVAGGTVLLLLFVISITTIIVIFLNRRRRRERRDLFTESWDTQKAPNNYRSPISTSQPTNQSMDDTREDIYVNYPTFSRRPKTRV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
88
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15762 As Substrate

Site PTM Type Enzyme
K167 Ubiquitination
Y249 Phosphorylation
T250 Phosphorylation
S290 Phosphorylation
T293 Phosphorylation
K295 Ubiquitination
S313 Phosphorylation
Y322 Phosphorylation P06241 (FYN)
Y325 Phosphorylation
S329 Phosphorylation P17252 (PRKCA)

Research Backgrounds

Function:

Involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell. Cell surface receptor for NECTIN2. Upon ligand binding, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Competes with PVRIG for NECTIN2-binding.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed by peripheral blood T-lymphocytes.

Subunit Structure:

Interacts with PVR and NECTIN2. Competes with PVRIG for NECTIN2-binding.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

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