MST1 Antibody - #DF8430

Fig. 4 Interaction of Integrin β1 and LN-5γ2 promoted TB and aggressiveness of CRC cells by activation of YAP. a The HCT-8 and SW480 cells were transfected with M02-ITGB1 or treated with exogenous recombinant LN-5γ2 (2 μg/mL) or both. The expression of FAK, p-FAK(Y397), MST1, LATS1, p-YAP, E-cadherin, N-Cadherin, and Vimentin was then examined by western blot analysis of the whole cell lysates, and the expression of YAP and TAZ in the nucleus was also examined. b In vitro inverse Matrigel invasion model showed that treatment with LN-5γ2 or overexpression of integrin β1 or both promoted TB in vitro, which was abolished when the expression of YAP was knocked down. Each bar represents the mean ± SD for biological triplicate experiments. c Immunohistochemical results of YAP in TB-positive CRC tissues. N/C, The nucleus/cytoplasmic ratio of YAP staining index. d Overexpression of Integrin β1 (by stable transfection of M02-ITGB1) or LN-5γ2 (by stable transfection of M02-LAMC2) or both promoted the tumor growth of HCT-8 xenografts, and the effects were abolished when the expression of YAP was knocked down. e Overexpression of integrin β1 or LN-5γ2 or both promoted the lung metastasis of HCT-8 xenografts, which was abolished when YAP was knocked down. Lung metastasis was evaluated by quantifying the percentage of lung metastatic area per lung tissue area of mice. (f) Increased expression of nucleus YAP can be found in the Integrin β1 and LN-5γ2 co-overexpressed xenograft tissues by IF and IHC analysis. *P < 0.05; **P < 0.01. NS not significant

Figure 3. FKN regulated polarization in IFN-γ-induced RAW264.7 cells via the Hippo signaling pathway. A, B, C, Western blot analysis and their respective quantitation were performed to assess the expression levels of FKN, MST1, YAP, p-YAP, p-TAZ, iNOS, TNF-α, Arg-1 and IL-10 protein in RAW264.7 cells(full-length blots are presented in Supplementary Figure1). Representative blots are shown. *P