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  • Product Name
    Phospho-ERK1/2 (Thr202/Tyr204) Antibody
  • Catalog No.
    AF1015
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity:
    Human, Mouse, Rat
  • Prediction:
    Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)
  • UniProt
  • Mol.Wt.
    42,44kDa
  • Concentration
    1mg/ml
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Product Information

Alternative Names:Expand▼

ERK 1; ERK; ERK-1; ERK1; ERT 2; ERT2; Extracellular Signal Regulated Kinase 1; Extracellular signal related kinase 1; Extracellular signal-regulated kinase 1; HGNC6877; HS44KDAP; HUMKER1A; Insulin Stimulated MAP2 Kinase; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP Kinase; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK; MAPK1; Mapk3; MGC20180; Microtubule Associated Protein 2 Kinase; Microtubule-associated protein 2 kinase; Mitogen Activated Protein Kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3; MK03_HUMAN; OTTHUMP00000174538; OTTHUMP00000174541; p44 ERK1; p44 MAPK; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM 3; PRKM3; Protein Kinase Mitogen Activated 3; ERK 2; ERK; ERK-2; ERT1; Extracellular Signal Regulated Kinase 2; Extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAPK 1; MAPK 2; Mapk1; MAPK2; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; MK01_HUMAN; P38; P40; P41; p42-MAPK; P42MAPK; PRKM1; PRKM2; protein kinase, mitogen-activated, 1; protein kinase, mitogen-activated, 2; protein tyrosine kinase ERK2;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, IF 1:200, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-ERK1/2 (Thr202/Tyr204) Antibody detects endogenous levels of ERK1/2 only when phosphorylated at Threonine 202/Tyrosine 204.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human ERK1/2 around the phosphorylation site of Thr202/Tyr204.

Uniprot:



>>Visit The Human Protein Atlas

Gene id:

Molecular Weight:

Observed Mol.Wt.: 42,44kDa.
Predicted Mol.Wt.: 44kDa.

Subcellular Location:

Nucleus.

Description:

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. Publishing reference https://www.spandidos-publications.com/10.3892/mmr.2016.5305/download http://www.sciencedirect.com/science/article/pii/S0306452216000464 http://www.sciencedirect.com/science/article/pii/S0171298514002782 https://www.spandidos-publications.com/etm/12/1/499/download

Sequence:
        10         20         30         40         50
MAAAAAQGGG GGEPRRTEGV GPGVPGEVEM VKGQPFDVGP RYTQLQYIGE
60 70 80 90 100
GAYGMVSSAY DHVRKTRVAI KKISPFEHQT YCQRTLREIQ ILLRFRHENV
110 120 130 140 150
IGIRDILRAS TLEAMRDVYI VQDLMETDLY KLLKSQQLSN DHICYFLYQI
160 170 180 190 200
LRGLKYIHSA NVLHRDLKPS NLLINTTCDL KICDFGLARI ADPEHDHTGF
210 220 230 240 250
LTEYVATRWY RAPEIMLNSK GYTKSIDIWS VGCILAEMLS NRPIFPGKHY
260 270 280 290 300
LDQLNHILGI LGSPSQEDLN CIINMKARNY LQSLPSKTKV AWAKLFPKSD
310 320 330 340 350
SKALDLLDRM LTFNPNKRIT VEEALAHPYL EQYYDPTDEP VAEEPFTFAM
360 370
ELDDLPKERL KELIFQETAR FQPGVLEAP

Background

Function:

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.

Post-translational Modifications:

Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204.

Subcellular Location:

Nucleus;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Binds both upstream activators and downstream substrates in multimolecular complexes. Found in a complex with at least BRAF, HRAS, MAP2K1/MEK1, MAPK3 and RGS14 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with ADAM15, ARRB2, CANX, DAPK1 (via death domain), HSF4, IER3, MAP2K1/MEK1, MORG1, NISCH, and SGK1. Interacts with PEA15 and MKNK2 (By similarity). MKNK2 isoform 1 binding prevents from dephosphorylation and inactivation (By similarity). Interacts with TPR. Interacts with CDKN2AIP. Interacts with HSF1 (via D domain and preferentially with hyperphosphorylated form); this interaction occurs upon heat shock (PubMed:10747973). Interacts with CAVIN4 (By similarity).

Similarity:

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Research Fields

Research Fields:

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.(View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Gap junction.(View pathway)
· Cellular Processes > Cell growth and death > Apoptosis.(View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Adherens junction.(View pathway)
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.(View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence.(View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.(View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway.(View pathway)
· Human Diseases > Cancers: Specific types > Pancreatic cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma.(View pathway)
· Human Diseases > Cancers: Specific types > Thyroid cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Bladder cancer.(View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Gastric cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Glioma.(View pathway)
· Human Diseases > Cancers: Specific types > Colorectal cancer.(View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.(View pathway)
· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.(View pathway)
· Human Diseases > Cancers: Specific types > Endometrial cancer.(View pathway)
· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.
· Human Diseases > Cancers: Specific types > Breast cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.(View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.(View pathway)
· Human Diseases > Cancers: Overview > Choline metabolism in cancer.(View pathway)
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Cancers: Specific types > Melanoma.(View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Substance dependence > Alcoholism.
· Human Diseases > Neurodegenerative diseases > Prion diseases.
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.
· Human Diseases > Cancers: Specific types > Prostate cancer.(View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.(View pathway)
· Organismal Systems > Immune system > Toll-like receptor signaling pathway.(View pathway)
· Organismal Systems > Immune system > IL-17 signaling pathway.(View pathway)
· Organismal Systems > Immune system > Platelet activation.(View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway.(View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.(View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.(View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.(View pathway)
· Organismal Systems > Endocrine system > Relaxin signaling pathway.
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.(View pathway)
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.(View pathway)
· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.
· Organismal Systems > Endocrine system > Insulin signaling pathway.(View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation.(View pathway)
· Organismal Systems > Endocrine system > Prolactin signaling pathway.(View pathway)
· Organismal Systems > Nervous system > Long-term potentiation.
· Organismal Systems > Nervous system > Neurotrophin signaling pathway.(View pathway)
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.(View pathway)
· Organismal Systems > Development > Osteoclast differentiation.(View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
· Organismal Systems > Development > Axon guidance.(View pathway)
· Organismal Systems > Nervous system > Long-term depression.
· Organismal Systems > Nervous system > Glutamatergic synapse.
· Organismal Systems > Nervous system > Cholinergic synapse.
· Organismal Systems > Environmental adaptation > Circadian entrainment.
· Organismal Systems > Immune system > NOD-like receptor signaling pathway.(View pathway)
· Organismal Systems > Endocrine system > Melanogenesis.
· Organismal Systems > Immune system > Chemokine signaling pathway.(View pathway)
· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.(View pathway)
· Organismal Systems > Nervous system > Serotonergic synapse.
· Organismal Systems > Immune system > B cell receptor signaling pathway.(View pathway)
· Organismal Systems > Endocrine system > Estrogen signaling pathway.(View pathway)

Western blot analysis of extracts from K562, using Phospho-ERK1/2 (Thr202/Tyr204) Antibody. The lane on the left was treated with blocking peptide.
Western blot analysis of ERK1/2 phosphorylation expression in HeLa whole cell lysates,The lane on the left was treated with the antigen-specific peptide.
AF1015 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF1015 at 1/200 staining Mouse liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF1015 at 1/200 staining Human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF1015 at 1/200 staining Human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
IHC analysis of paraffin-embedded human testis tissue using Phospho-ERK1/2 (Thr202/Tyr204) Antibody at dilution of 1:100.
AF1015 staining lovo cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
ELISA analysis of AF1015 showing specificity to Phospho-ERK1/2 (Thr202/Tyr204) peptide. Peptides concentration: 1ug/ml.
P-peptide: phospho-peptide; N-peptide: non-phospho-peptide.

Reference Citations:

1). Liu Y et al. LncRNA-TCONS_00034812 in cell proliferation and apoptosis of pulmonary artery smooth muscle cells and its mechanism. J Cell Physiol 2018 Jun;233(6):4801-4814 (PubMed: 29150946) [IF=4.522]

2). Liu Y et al. LncRNA-TCONS_00034812 in cell proliferation and apoptosis of pulmonary artery smooth muscle cells and its mechanism. J Cell Physiol 2018 Jun;233(6):4801-4814 (PubMed: 29150946) [IF=4.522]

3). Liu Y et al. LncRNA-TCONS_00034812 in cell proliferation and apoptosis of pulmonary artery smooth muscle cells and its mechanism. J Cell Physiol 2018 Jun;233(6):4801-4814 (PubMed: 29150946) [IF=4.522]

4). Fan H et al. The in vitro and in vivo anti-inflammatory effect of osthole, the major natural coumarin from Cnidium monnieri (L.) Cuss, via the blocking of the activation of the NF-κB and MAPK/p38 pathways. Phytomedicine 2019 Feb 18;58:152864 (PubMed: 30878874) [IF=4.180]

5). Lu G et al. Inhibition of the cyclophilin A-CD147 interaction attenuates right ventricular injury and dysfunction after acute pulmonary embolism in rats. J Biol Chem 2018 Aug 3;293(31):12199-12208 (PubMed: 29914983) [IF=4.106]

6). Lu G et al. Inhibition of the cyclophilin A-CD147 interaction attenuates right ventricular injury and dysfunction after acute pulmonary embolism in rats. J Biol Chem 2018 Aug 3;293(31):12199-12208 (PubMed: 29914983) [IF=4.106]

7). Xie J et al. 5-aminolevulinic acid photodynamic therapy reduces HPV viral load via autophagy and apoptosis by modulating Ras/Raf/MEK/ERK and PI3K/AKT pathways in HeLa cells. J Photochem Photobiol B 2019 Mar 21;194:46-55 (PubMed: 30925276) [IF=4.067]

8). Yang F et al. Ligustilide, a major bioactive component of Angelica sinensis, promotes bone formation via the GPR30/EGFR pathway. Sci Rep 2019 May 6;9(1):6991 (PubMed: 31061445) [IF=4.011]

9). Zhang S et al. A novel, highly-water-soluble apigenin derivative provides neuroprotection following ischemia in male rats by regulating the ERK/Nrf2/HO-1 pathway. Eur J Pharmacol 2019 Mar 14 (PubMed: 30880180)

10). Zhang X et al. Neuroprotective Effect of Modified Xijiao Dihuang Decoction against Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury in PC12 Cells: Involvement of TLR4-MyD88/NF-κB Signaling Pathway. Evid Based Complement Alternat Med 2017;2017:3848595 (PubMed: 29234386)

11). Wu Z et al. MFAP5 promotes tumor progression and bone metastasis by regulating ERK/MMP signaling pathways in breast cancer. Biochem Biophys Res Commun 2018 Apr 6;498(3):495-501 (PubMed: 29526753)

12). Wang W et al. Umbilical cord‑derived mesenchymal stem cells can inhibit the biological functions of melanoma A375 cells. Oncol Rep 2018 Jul;40(1):511-517 (PubMed: 29767256)

13). Wu CX et al. Electroacupuncture exerts neuroprotective effects on ischemia/reperfusion injury in JNK knockout mice: the underlying mechanism. Neural Regen Res 2018 Sep;13(9):1594-1601 (PubMed: 30127120)

14). Huang C et al. Estrogen regulates excitatory amino acid carrier 1 (EAAC1) expression through sphingosine kinase 1 (SphK1) transacting FGFR-mediated ERK signaling in rat C6 astroglial cells. Neuroscience 2016 Apr 5;319:9-22 (PubMed: 26804240)

Application: WB    Species:rat;    Sample:rat

Fig. 4. FGFR- ERK signaling pathway is involved in FGF2-induced EAAC1 expression. (A) C6 cells were incubated with FGF2 (10 ng/mL) for different durations. (B, C) Before incubation with FGF2 (10 ng/mL) for 15 min (B) or 24 h (C), cells were pretreated with the FGFR kinase inhibitor SU5402 (SU; 25 lM, 5 min) or the ERK inhibitor U0126 (U0; 10 lM, 1 h). Phosphorylation of FRS2a or ERK1/2 (A and B, respectively), and EAAC1 expression (C) were analyzed by Western blots. Data are shown as mean ± SEM from three independent experiments. * p < 0.05; **p < 0.01 versus the control group. # p < 0.05; ##p < 0.01 versus the FGF2 alone group.


15). Liao ZL et al. Salidroside protects PC-12 cells against amyloid β-induced apoptosis by activation of the ERK1/2 and AKT signaling pathways. Int J Mol Med 2019 Feb 1 (PubMed: 30720058)

16). Cui C et al. Hypoxic postconditioning attenuates apoptosis via inactivation of adenosine A2a receptor through NDRG3-Raf-ERK pathway. Biochem Biophys Res Commun 2017 Sep 16;491(2):277-284 (PubMed: 28743501)

Application: WB    Species:human;    Sample:Not available

Fig.2 Protein Expression of NDRD3-Raf-ERK pathway, A2a receptors, Cytochrome C.


17). Chai L et al. Biological functions of lung cancer cells are suppressed in co-culture with mesenchymal stem cells isolated from umbilical cord. Exp Ther Med 2018 Jan;15(1):1076-1080 (PubMed: 29399109)

18). Liu Y et al. (3R, 7R)-7-Acetoxyl-9-Oxo-de-O-Methyllasiodiplodin, a Secondary Metabolite of Penicillium Sp., Inhibits LPS-Mediated Inflammation in RAW 264.7 Macrophages through Blocking ERK/MAPKs and NF-κB Signaling Pathways. Inflammation 2019 Apr 23 (PubMed: 31011928)

19). Tang Q et al. Ferroptosis is newly characterized form of neuronal cell death in response to arsenite exposure. Neurotoxicology 2018 Jul;67:27-36 (PubMed: 29678591)

20). Jin X et al. 11-O-acetylcyathatriol inhibits MAPK/p38-mediated inflammation in LPS-activated RAW 264.7 macrophages and has a protective effect on ethanol-induced gastric injury. Mol Med Rep 2016 Jul;14(1):874-80 (PubMed: 27222252)

Application: WB    Species:mouse;    Sample:Not available

Figure 3. Effects of 11‑O‑acetylcyathatriol on the protein expression levels of iNOS and COX‑2. The RAW 264.7 cells were treated by 1 µg/ml of LPS with indicated concentrations of 11-O‑acetylcyathatriol (12.5, 25, 50 and 100 µM) for 24 h, and the expression levels of (A) iNOS and COX‑2 were detected using western blot analysis. (B) Effects of 11‑O-acetylcyathatriol on the phosphorylation of ERK1/2, JNK and p38 proteins. RAW 264.7 cells were treated with 1 µg/ml LPS with 11‑O‑acetylcyathatriol (12.5, 25, 50 and 100 µM) for 30 min, and the protein expression levels of p‑ERK1/2, p‑JNK and p‑p38 were detected using western blot analysis. (C) Effects of 11‑O-acetylcyathatriol on the protein degradation of IκB-α. RAW 264.7 cells were treated with 1 µg/ml of LPS with 11-O‑acetylcyathatriol (12.5, 25, 50 and 100 µM) for 10 min, and the protein expression of IκB-α was detected using western blot analysis. iNOS, inducible nitric oxide synthase; COX‑2, cyclooxygenase‑2; LPS, lipopolysaccharide; ERK, extracellular signal‑regulated kinase; JNK, c‑Jun N‑terminal kinase; IκB-α, inhibitor of nuclear factor-κB-α; p‑, phosphorylated.


21). Deng LJ et al. Oxabicycloheptene Sulfonate Protects Against β-Amyloid-induced Toxicity by Activation of PI3K/Akt and ERK Signaling Pathways Via GPER1 in C6 Cells. Neurochem Res 2017 Aug;42(8):2246-2256 (PubMed: 28374135)

Application: WB    Species:rat;    Sample:C6 Cells

Fig. 3 OBHS and RAL increase Akt and ERK phosphorylation through GPER1. a Akt and ERK inhibitors attenuated the pro-survival effects of OBHS and RAL against Aβ toxicity. Cells were pretreated with specific inhibitors LY294002 (LY, 20  μM), U0126 (U,10 μM) for one hour and then incubated with OBHS (1 μM) or RAL (1 μM) for 45 min, after Aβ treatment for an additional 24 h. Cell viability was measured by MTT method. b,c C6 cells were preincubated with PI3K/Akt inhibitor LY (b) or ERK inhibitor U (c) for one hour, followed by OBHS (1 μM) or RAL (1 μM) treatment for 15 min. d GPER1 antagonist G15 (10  μM) was added to the media for 24  h, before OBHS (1  μM) or RAL (1  μM) treatment for 15  min of C6 cells. The proteins were analyzed by the western blot method. The data were shown as the mean±SD from three independent experiments.


22). Yuan Q et al. The role of adenosine in up-regulation of p38 MAPK and ERK during limb ischemic preconditioning-induced brain ischemic tolerance. Brain Res 2018 Nov 13 (PubMed: 30445027)

23). Zhang T et al. Interaction with tumor‑associated macrophages promotes PRL‑3‑induced invasion of colorectal cancer cells via MAPK pathway‑induced EMT and NF‑κB signaling‑induced angiogenesis. Oncol Rep 2019 Mar 7 (PubMed: 30864736)

24). Li H et al. Dysifragilone A inhibits LPS‑induced RAW264.7 macrophage activation by blocking the p38 MAPK signaling pathway. Mol Med Rep 2018 Jan;17(1):674-682 (PubMed: 29115475)

25). Han Y et al. Mechanosensitive ion channel Piezo1 promotes prostate cancer development through the activation of the Akt/mTOR pathway and acceleration of cell cycle. Int J Oncol 2019 Jul 15 (PubMed: 31322184)

26). Zhang Q et al. Anti-inflammatory action of ambuic acid, a natural product isolated from the solid culture of Pestalotiopsis neglecta, through blocking ERK/JNK mitogen-activated protein kinase signaling pathway. Exp Ther Med 2018 Aug;16(2):1538-1546 (PubMed: 30116402)

27). Peng J et al. miR-221 negatively regulates inflammation and insulin sensitivity in white adipose tissue by repression of sirtuin-1 (SIRT1). J Cell Biochem 2018 Aug;119(8):6418-6428 (PubMed: 29236311)

28). Song T et al. GPR120 promotes adipogenesis through intracellular calcium and extracellular signal-regulated kinase 1/2 signal pathway. Mol Cell Endocrinol 2016 Oct 15;434:1-13 (PubMed: 27302893)

Application: WB    Species:Not available;    Sample:Not available


29). Peng J et al. MiR-377 promotes white adipose tissue inflammation and decreases insulin sensitivity in obesity via suppression of sirtuin-1 (SIRT1). Oncotarget 2017 Jul 31;8(41):70550-70563 (PubMed: 29050301)

Application: WB    Species:mouse;    Sample:Not available

Figure 4: MiR-377 promotes inflammation and insulin-resistance in mature 3T3-L1 cells. After transfection with 100 nM miR-377 mimics or inhibitor for 24 h, differentiated 3T3-L1 adipocytes were treated with 10 ng/ml TNFα for 24 h and then stimulated with 100 nM insulin for 15 min. Cells were then harvested for real-time PCR and immunoblotting analyses. (A) The effect of miR-377 overexpression on inflammation-related gene expression. *P < 0.05, **P < 0.01 vs. NC 0.1% BSA; ##P < 0.01 vs. NC 10 ng/ml TNFα; ns, not signifcant (n = 3). (B) The effect of miR-377 inhibition on inflammation-related gene expression under conditions of TNFα-induced insulin-resistance. ##P < 0.01 vs. NC 10 ng/ml TNFα(n = 3). (C) The effect of miR-377 overexpression on JNK phosphorylation under conditions of TNFα-induced insulin-resistance. *P < 0.05 vs. NC without insulin; #P < 0.05 vs. NC with insulin (n = 3). (D and E) The effect of miR-377 overexpression on AKT and ERK phosphorylation. *P < 0.05 vs. NC with 0.1% BSA and insulin; #P < 0.05 vs. NC with 10 ng/ml TNFα and insulin (n = 3). (F) The effect of miR-377 inhibition on JNK phosphorylation under conditions of TNFα-induced insulin-resistance. *P < 0.05 vs. NC without insulin; #P < 0.05 vs. NC with insulin (n = 3). (G and H) The effect of miR-377 inhibition on AKT and ERK phosphorylation. *P < 0.05 vs. NC with 0.1% BSA and insulin; #P < 0.05 vs. NC with 10 ng/ml TNFα and insulin (n = 3).


30). Lin F et al. GTSE1 is involved in breast cancer progression in p53 mutation-dependent manner. J Exp Clin Cancer Res 2019 Apr 8;38(1):152 (PubMed: 30961661)

31). Huang YX et al. Ovostatin 2 knockdown significantly inhibits the growth, migration, and tumorigenicity of cutaneous malignant melanoma cells. PLoS One 2018 Apr 23;13(4):e0195610 (PubMed: 29684087)

32). Huikun Xu et al. Chemerin promotes the viability and migration of human placental microvascular endothelial cells and activates MAPK/AKT signaling . Int J Clin Exp Med 2018;11(2):721-727

33). Su Z et al. HMGB1 modulates Lewis cell autophagy and promotes cell survival via RAGE-HMGB1-Erk1/2 positive feedback during nutrient depletion. Immunobiology 2015 May;220(5):539-44 (PubMed: 25578401)

Application: WB    Species:Not available;    Sample:Not available


34). Li ZH et al. miR-29a regulated ER-positive breast cancer cell growth and invasion and is involved in the insulin signaling pathway. Oncotarget 2017 May 16;8(20):32566-32575 (PubMed: 28427228)

Application: WB    Species:human;    Sample:MCF-7

Figure 5: Effect of miR-29a on the insulin signaling pathways. (A) IGF-1R, CDC42, p85α, p-ERK and ERK mRNA levels were determined by qPCR in ER-positive cells transfected with a miR-29a mimic or miR-29a inhibitor. (B, C) Western blotting analysis was performed to monitor IGF-1R, CDC42, p85α, p-ERK and ERK expression in ER-positive cells transfected with a miR-29a mimic or miR-29a inhibitor. The results showed that ERK, CDC42 and p85α are the target genes of miR-29a; however, miR-29a promotes breast cancer cell growth and proliferation mainly by activating ERK phosphorylation.


35). Liu X et al. MicroRNA-370 inhibits the growth and metastasis of lung cancer by down-regulating epidermal growth factor receptor expression. Oncotarget 2017 Oct 4;8(50):88139-88151 (PubMed: 29152147)

Application: WB    Species:human;    Sample:Not available

Figure 4: Induction of miR-370 over-expression reduces EGFR and HIF-1α expression and inhibits the ERK and AKT phosphorylation in XWLC-05 and H157 cells. XWLC-05 and H157 cells were transfected with miR-370 mimics, miR-370 inhibitor or corresponding controls for 24 h. The relative levels of EGFR, HIF-1α, ERK, AKT expression, ERK and AKT phosphorylation were determined by Western blot assays. Data are representative images or expressed as the means ± SEM of each group of cells from three separate experiments. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.


36). Xin H et al. Chemokine CXCL3 mediates prostate cancer cells proliferation, migration and gene expression changes in an autocrine/paracrine fashion. Int Urol Nephrol 2018 May;50(5):861-868 (PubMed: 29524043)

37). Jin X et al. Tiliroside, the major component of Agrimonia pilosa Ledeb ethanol extract, inhibits MAPK/JNK/p38-mediated inflammation in lipopolysaccharide-activated RAW 264.7 macrophages. Exp Ther Med 2016 Jul;12(1):499-505 (PubMed: 27347085)

Application: WB    Species:mouse;    Sample:Not available

Figure 7. Effect of tiliroside on the phosphorylation of mitogen‑activated protein kinase‑ERK/JNK/p38 proteins. (A) RAW 264.7 cells were treated with LPS 1 µg/ml with or without tiliroside (12.5, 25, 50, and 100 µM) for 45 min and the expression of p‑ERK1/2, p‑JNK and p‑p38 was assessed by western blot analysis. Detection of β‑actin was conducted to confirm the equal loading of proteins. Densitometric analysis of p‑ERK1/2 (B) p‑JNK (C) and p‑p38 (D) expression represent the mean±standard deviation of three separate experiments. Data were normalized with respect to β‑actin levels. **P


38). Zhao H et al. CCL17-CCR4 axis promotes metastasis via ERK/MMP13 pathway in bladder cancer. J Cell Biochem 2018 Sep 19 (PubMed: 30230587)

39). Xie XC et al. Relaxin Attenuates Contrast-Induced Human Proximal Tubular Epithelial Cell Apoptosis by Activation of the PI3K/Akt Signaling Pathway In Vitro. Biomed Res Int 2017;2017:2869405 (PubMed: 28540295)

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Catalog Number :

AF1015-BP

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Rabbit
100%
Zebrafish
100%
Pig
100%
Sheep
100%
Horse
100%
Bovine
100%
Chicken
0%
Dog
0%
Xenopus
0%
High similarity Medium similarity Low similarity No similarity
P27361/P28482 as Substrate
Site PTM Type Enzyme
A2 Acetylation
K32 Ubiquitination
S58 Phosphorylation
K72 Ubiquitination
T80 Phosphorylation
Y130 Phosphorylation
K155 Ubiquitination
S159 Phosphorylation
S170 Phosphorylation
K181 Acetylation
K181 Ubiquitination
T198 Phosphorylation
T202 Phosphorylation Q02750 (MAP2K1) , P36507 (MAP2K2)
Y204 Phosphorylation P27361 (MAPK3) , O60674 (JAK2) , Q02750 (MAP2K1) , P07949 (RET) , P06239 (LCK) , P36507 (MAP2K2)
T207 Phosphorylation Q02750 (MAP2K1) , P27361 (MAPK3)
Y210 Phosphorylation Q02750 (MAP2K1)
R211 Methylation
S219 Phosphorylation
K220 Ubiquitination
Y222 Phosphorylation
T223 Phosphorylation
S265 Phosphorylation
K287 Ubiquitination
K289 Ubiquitination
K294 Ubiquitination
K298 Ubiquitination
K302 Methylation
K302 Ubiquitination
R309 Methylation
K317 Ubiquitination
K357 Ubiquitination
K361 Methylation
K361 Ubiquitination
Site PTM Type Enzyme
A2 Acetylation
Y25 Phosphorylation
S29 Phosphorylation O00141 (SGK1)
Y36 Phosphorylation
Y43 Phosphorylation
K55 Ubiquitination
T63 Phosphorylation
C65 S-Nitrosylation
K99 Ubiquitination
Y113 Phosphorylation
K138 Ubiquitination
S142 Phosphorylation
K151 Ubiquitination
K164 Ubiquitination
C166 S-Nitrosylation
T181 Phosphorylation
T185 Phosphorylation Q02750 (MAP2K1) , P36507 (MAP2K2)
Y187 Phosphorylation P28482 (MAPK1) , P07949 (RET) , O60674 (JAK2) , Q02750 (MAP2K1) , P36507 (MAP2K2)
T190 Phosphorylation P28482 (MAPK1)
Y193 Phosphorylation
R194 Methylation
S202 Phosphorylation
K203 Ubiquitination
Y205 Phosphorylation
T206 Phosphorylation
S246 Phosphorylation P68400 (CSNK2A1) , P28482 (MAPK1)
S248 Phosphorylation P68400 (CSNK2A1)
K259 Ubiquitination
Y263 Phosphorylation
K270 Ubiquitination
K272 Ubiquitination
S284 Phosphorylation
K285 Ubiquitination
K292 Ubiquitination
T295 Phosphorylation
K300 Ubiquitination
K330 Ubiquitination
K340 Ubiquitination
K344 Ubiquitination
S360 Phosphorylation
P27361/P28482 as PTM Enzyme
Substrate Site Source
C9JLW8 (MCRIP1) S21 Calculation
C9JLW8 (MCRIP1) T30 Calculation
O00213 (APBB1) S175 Signor
O00213 (APBB1) S287 Signor
O00213 (APBB1) S347 Signor
O00213 (APBB1) T709 Signor
O00429 (DNM1L) S616 Calculation
O14994 (SYN3) S470 neXtProt, phospho.ELM, Signor
O43521 (BCL2L11) S69 Signor
O43524 (FOXO3) S294 Signor
O43524 (FOXO3) S344 Signor
O43524 (FOXO3) S425 Signor
O43561-2 (LAT) T155 Calculation
O43561 (LAT) T184 Signor
O60244 (MED14) S986 Calculation
O60331 (PIP5K1C) S650 Calculation
O60674 (JAK2) S523 Signor
O75030-9 (MITF) S73 Calculation
O75581 (LRP6) S1490 Calculation
O75581 (LRP6) T1572 Calculation
O75582 (RPS6KA5) S360 Signor
O75582-2 (RPS6KA5) S376 HPRD
O75582-1 (RPS6KA5) T581 Signor
O75582 (RPS6KA5) T700 neXtProt
O95644 (NFATC1) S172 Signor
O95997 (PTTG1) S165 Signor
P00533 (EGFR) T693 Signor
P01100 (FOS) T232 phospho.ELM, Signor
P01100 (FOS) T325 phospho.ELM, Signor
P01100 (FOS) T331 phospho.ELM, Signor
P01100 (FOS) S374 Signor
P02686 (MBP) S96 neXtProt
P02686 (MBP) T232 Signor
P03372 (ESR1) S104 Signor
P03372 (ESR1) S106 Signor
P03372 (ESR1) S118 Signor
P04049 (RAF1) S289 Signor
P04049 (RAF1) S296 Signor
P04049 (RAF1) S301 Signor
P04150 (NR3C1) S226 Signor
P04637 (TP53) S15 Signor
P04637 (TP53) S392 Calculation
P05412 (JUN) S63 Signor
P05412 (JUN) S73 Signor
P05787-1 (KRT8) S74 HPRD
P05787 (KRT8) S432 Signor
P06239 (LCK) S42 phospho.ELM, Signor
P06239 (LCK) S59 Signor
P06401 (PGR) S294 Signor
P07101-3 (TH) S31 Calculation
P07101-4 (TH) S35 Calculation
P07101-2 (TH) S58 Calculation
P07101 (TH) S62 Signor
P08047 (SP1) S59 Signor
P08047 (SP1) T453 Signor
P08047 (SP1) T739 Signor
P09917 (ALOX5) S664 neXtProt
P10242 (MYB) S532 Signor
P10275 (AR) S516 Calculation
P10275 (AR) S651 Calculation
P10415 (BCL2) T56 Signor
P10415 (BCL2) S70 neXtProt, Signor
P10415 (BCL2) T74 Signor
P10415 (BCL2) S87 Calculation
P10636-8 (MAPT) S46 Calculation
P10636-8 (MAPT) T50 Calculation
P10636-8 (MAPT) T153 Calculation
P10636-8 (MAPT) T175 Calculation
P10636-8 (MAPT) T181 Calculation
P10636-8 (MAPT) S199 Calculation
P10636-8 (MAPT) S202 Calculation
P10636-8 (MAPT) T205 Calculation
P10636-8 (MAPT) T212 Calculation
P10636-8 (MAPT) T217 Calculation
P10636-8 (MAPT) T231 Calculation
P10636-8 (MAPT) S235 Calculation
P10636-8 (MAPT) S396 Calculation
P10636-8 (MAPT) S404 Calculation
P10636-8 (MAPT) S422 Calculation
P10828 (THRB) S142 Signor
P11362 (FGFR1) S777 Signor
P11388 (TOP2A) S1213 Calculation
P11388 (TOP2A) S1247 Calculation
P11388 (TOP2A) S1354 Calculation
P11388 (TOP2A) S1361 Calculation
P11388 (TOP2A) S1393 Calculation
P12980 (LYL1) S36 Calculation
P13631 (RARG) S77 neXtProt
P13631 (RARG) S79 Calculation
P14598 (NCF1) S345 Signor
P14598 (NCF1) S348 Calculation
P14784 (IL2RB) T476 Calculation
P14921 (ETS1) T38 HPRD, neXtProt, Signor
P15036 (ETS2) T72 neXtProt
P15056 (BRAF) S151 Calculation
P15056 (BRAF) T401 Calculation
P15056 (BRAF) T753 Signor
P15336 (ATF2) T69 Signor
P15336 (ATF2) T71 Signor
P15923-1 (TCF3) T355 HPRD
P15976 (GATA1) S26 neXtProt
P16144 (ITGB4) S1356 Calculation
P16220 (CREB1) S133 Calculation
P16949 (STMN1) S25 Signor
P16949 (STMN1) S38 Signor
P17302 (GJA1) S255 HPRD, neXtProt, Signor
P17302 (GJA1) S279 HPRD, neXtProt, Signor
P17302 (GJA1) S282 HPRD, neXtProt, Signor
P17480 (UBTF) T117 HPRD, neXtProt, phospho.ELM, Signor
P17480 (UBTF) T201 HPRD, neXtProt, phospho.ELM, Signor
P17535 (JUND) S100 Signor
P17542-1 (TAL1) S122 Signor
P17600 (SYN1) S62 neXtProt
P17600 (SYN1) S67 neXtProt
P17600 (SYN1) S551 neXtProt
P17655 (CAPN2) S50 Signor
P17676 (CEBPB) T235 Signor
P17861 (XBP1) S68 Calculation
P17861 (XBP1) S181 Calculation
P18545 (PDE6G) T22 HPRD
P19419 (ELK1) S324 Signor
P19419 (ELK1) T336 Signor
P19419 (ELK1) S383 Signor
P19419 (ELK1) S389 Signor
P19419 (ELK1) S422 Signor
P19793 (RXRA) S260 Signor
P20339 (RAB5A) S123 Calculation
P23396 (RPS3) T42 Signor
P23443 (RPS6KB1) S434 neXtProt
P24928 (POLR2A) S1619 phospho.ELM, Signor
P24928 (POLR2A) S1626 Signor
P24928 (POLR2A) S1647 Signor
P24928 (POLR2A) S1654 Signor
P24928 (POLR2A) S1668 Signor
P24928 (POLR2A) S1675 Signor
P24928 (POLR2A) S1696 Signor
P24928 (POLR2A) S1717 Signor
P24928 (POLR2A) S1724 Signor
P24928 (POLR2A) S1738 Signor
P24928 (POLR2A) S1766 Signor
P24928 (POLR2A) S1787 Signor
P24928 (POLR2A) S1864 Signor
P24928 (POLR2A) S1871 Signor
P24928 (POLR2A) S1878 Signor
P24928 (POLR2A) S1892 Signor
P24928 (POLR2A) S1899 Signor
P24928 (POLR2A) S1913 Signor
P24928 (POLR2A) S1920 Signor
P24928 (POLR2A) S1927 Signor
P24928 (POLR2A) S1934 Signor
P24928 (POLR2A) S1944 Signor
P24928 (POLR2A) S1951 Signor
P24941 (CDK2) T160 Calculation
P25098 (GRK2) S670 HPRD, neXtProt, Signor
P27361-3 (MAPK3) T202 HPRD
P27361 (MAPK3) Y204 Calculation
P27361 (MAPK3) T207 Signor, UniProt
P27708 (CAD) T456 Signor
P27824 (CANX) S583 Calculation
P28324 (ELK4) T361 neXtProt
P28324 (ELK4) T366 neXtProt
P28324 (ELK4) S381 neXtProt
P28324 (ELK4) S387 neXtProt
P28324 (ELK4) T420 neXtProt
P28324 (ELK4) S425 neXtProt
P28562 (DUSP1) S296 Signor
P28562 (DUSP1) S323 Signor
P28562 (DUSP1) S359 Signor
P28562 (DUSP1) S364 HPRD, phospho.ELM, Signor
P28698 (MZF1) S256 neXtProt
P28698 (MZF1) S274 neXtProt
P28698 (MZF1) S294 neXtProt
P29353 (SHC1) S36 neXtProt
P29590-4 (PML) T28 Calculation
P29590-4 (PML) S36 Calculation
P29590-4 (PML) S38 Calculation
P29590-4 (PML) S40 Calculation
P29590-4 (PML) S527 Calculation
P29590-4 (PML) S530 Signor
P30307 (CDC25C) S216 Calculation
P31645 (SLC6A4) T616 Calculation
P32121 (ARRB2) S14 Calculation
P32121 (ARRB2) T276 Calculation
P33076 (CIITA) S280 Signor
P33076 (CIITA) S288 Signor
P33981 (TTK) S821 Calculation
P35228 (NOS2) S745 Signor
P35236-2 (PTPN7) T105 HPRD
P35236-2 (PTPN7) S132 HPRD
P35568 (IRS1) S616 Signor
P35658 (NUP214) S1710 Calculation
P35658 (NUP214) S1809 Calculation
P36956 (SREBF1) S117 Signor
P36956-4 (SREBF1) S147 HPRD
P37231 (PPARG) S112 Signor
P40763 (STAT3) Y705 Signor
P40763-2 (STAT3) S726 HPRD
P40763 (STAT3) S727 Signor
P41182 (BCL6) S333 Signor
P41182 (BCL6) S343 Signor
P41212 (ETV6) S213 Signor
P41212 (ETV6) S257 Calculation
P42224 (STAT1) S727 Calculation
P42229 (STAT5A) S780 Signor
P42702 (LIFR) S1044 Signor
P43364 (MAGEA11) S174 Signor
P43694 (GATA4) S105 Calculation
P46527 (CDKN1B) T187 Signor
P47712 (PLA2G4A) S505 Signor
P49023-2 (PXN) S83 Calculation
P49023-2 (PXN) S126 Calculation
P49023 (PXN) S130 Calculation
P49137 (MAPKAPK2) T222 neXtProt
P49137 (MAPKAPK2) T334 neXtProt
P49407-2 (ARRB1) S404 HPRD
P49407-1 (ARRB1) S412 Signor
P49418 (AMPH) S293 Signor
P49418 (AMPH) S295 Signor
P49585 (PCYT1A) S315 Signor
P49675 (STAR) S233 neXtProt
P49715 (CEBPA) S21 Signor
P49790 (NUP153) S257 Calculation
P49790 (NUP153) S320 Calculation
P49790 (NUP153) S334 Calculation
P49790 (NUP153) S338 Calculation
P49790 (NUP153) T369 Calculation
P49790 (NUP153) T388 Signor
P49790 (NUP153) T413 Calculation
P49790 (NUP153) S516 Calculation
P49790 (NUP153) S522 Calculation
P49790 (NUP153) S529 Signor
P49815 (TSC2) S664 Calculation
P49841 (GSK3B) T43 Signor
P50616 (TOB1) S152 Signor
P50616 (TOB1) S154 Signor
P50616 (TOB1) S164 Signor
P51168 (SCNN1B) T615 neXtProt
P51170 (SCNN1G) T622 neXtProt
P51812 (RPS6KA3) S227 Signor
P51812 (RPS6KA3) T577 phospho.ELM, Signor
P53004 (BLVRA) S230 Calculation
P53355 (DAPK1) S734 Calculation
P53805-4 (RCAN1) S32 HPRD
P53805-2 (RCAN1) S112 HPRD
P53805 (RCAN1) S167 HPRD, neXtProt
P55211 (CASP9) T125 Signor
P56270 (MAZ) T72 Signor
P61586 (RHOA) S88 Calculation
P61586 (RHOA) T100 Calculation
P61978 (HNRNPK) S284 Signor
P61978 (HNRNPK) S353 Calculation
P63000 (RAC1) T108 Calculation
P68431 (HIST1H3J) S29 neXtProt
P78347-2 (GTF2I) S627 Calculation
P78347-2 (GTF2I) S633 Calculation
P78347 (GTF2I) S668 Signor
P78347 (GTF2I) S674 Signor
P78536 (ADAM17) T735 Signor
P84022 (SMAD3) S204 Calculation
P84243 (H3F3B) S29 Signor
Q00613-1 (HSF1) S307 Signor
Q00975 (CACNA1B) S411 neXtProt
Q00975 (CACNA1B) S446 neXtProt
Q01196-8 (RUNX1) S276 Calculation
Q01196-8 (RUNX1) S293 Calculation
Q01196-8 (RUNX1) T300 Calculation
Q01196-8 (RUNX1) S303 Calculation
Q01196-8 (RUNX1) S462 Calculation
Q01844 (EWSR1) T79 Signor
Q01860 (POU5F1) S111 Signor
Q01892 (SPIB) T56 HPRD, phospho.ELM, Signor
Q02447 (SP3) S73 Signor
Q02641 (CACNB1) S161 neXtProt
Q02641 (CACNB1) S348 neXtProt
Q02750 (MAP2K1) T286 Calculation
Q02750 (MAP2K1) T292 Calculation
Q02750 (MAP2K1) T386 neXtProt
Q04637-8 (EIF4G1) S1232 Calculation
Q05209 (PTPN12) S571 Calculation
Q05397 (PTK2) S910 Calculation
Q05469-1 (LIPE) T891 HPRD
Q05682-4 (CALD1) S534 Calculation
Q05682 (CALD1) S759 Signor
Q05682 (CALD1) S789 Signor
Q07812 (BAX) T167 Calculation
Q07820 (MCL1) T92 Calculation
Q07820 (MCL1) T163 Signor
Q07866 (KLC1) S460 Signor
Q07869-1 (PPARA) S12 HPRD, Signor
Q07869-1 (PPARA) S21 HPRD, Signor
Q07889 (SOS1) S1132 Signor
Q07889 (SOS1) S1167 Signor
Q07889 (SOS1) S1178 Signor
Q07889 (SOS1) S1193 Signor
Q08499-2 (PDE4D) S579 Calculation
Q08499 (PDE4D) S715 Signor
Q09472 (EP300) S1038 Calculation
Q09472 (EP300) S2039 Calculation
Q12772 (SREBF2) S432 Signor
Q12772 (SREBF2) S455 Signor
Q12800 (TFCP2) S291 Signor
Q12800 (TFCP2) S309 Signor
Q12929 (EPS8) S625 neXtProt
Q12929 (EPS8) T629 neXtProt
Q13153 (PAK1) T292 Signor
Q13164 (MAPK7) T733 Calculation
Q13322 (GRB10) S150 phospho.ELM, Signor
Q13322 (GRB10) S476 phospho.ELM, Signor
Q13362 (PPP2R5C) S337 Signor
Q13480-2 (GAB1) T312 HPRD
Q13480-2 (GAB1) S381 HPRD
Q13480-2 (GAB1) S454 HPRD
Q13480-1 (GAB1) T476 HPRD
Q13480-1 (GAB1) S551 HPRD
Q13480-1 (GAB1) S567 HPRD
Q13480-2 (GAB1) S581 HPRD
Q13480-2 (GAB1) S597 HPRD
Q13485 (SMAD4) T277 phospho.ELM, Signor
Q13522 (PPP1R1A) S67 neXtProt
Q13541 (EIF4EBP1) S65 Calculation
Q13586 (STIM1) S575 Calculation
Q13586 (STIM1) S608 Calculation
Q13586 (STIM1) S621 Calculation
Q13614 (MTMR2) S58 Calculation
Q13950 (RUNX2) S24 Calculation
Q13950 (RUNX2) S28 Calculation
Q13950 (RUNX2) S43 Calculation
Q13950 (RUNX2) S275 Calculation
Q13950 (RUNX2) S294 Signor
Q13950 (RUNX2) S312 Signor
Q13950 (RUNX2) S314 Calculation
Q13950 (RUNX2) S340 Calculation
Q13950 (RUNX2) S503 Calculation
Q14005 (IL16) S845 Signor
Q14160 (SCRIB) T553 neXtProt
Q14160 (SCRIB) S1448 neXtProt
Q14247 (CTTN) S405 Signor
Q14247 (CTTN) S418 Signor
Q14790 (CASP8) S387 Signor
Q14934 (NFATC4) S676 Signor
Q15046 (KARS) S207 Signor
Q15366 (PCBP2) S173 Calculation
Q15366 (PCBP2) S189 Calculation
Q15366 (PCBP2) T213 Calculation
Q15366 (PCBP2) S272 Calculation
Q15596 (NCOA2) S736 Calculation
Q15648 (MED1) T1032 Signor
Q15648 (MED1) T1457 Signor
Q15672 (TWIST1) S68 Signor
Q15746 (MYLK) S1779 Calculation
Q15788 (NCOA1) T1179 Signor
Q15788 (NCOA1) S1185 Signor
Q15796 (SMAD2) T8 Signor
Q15796 (SMAD2) T220 Signor
Q15796 (SMAD2) S245 Signor
Q15796 (SMAD2) S250 Signor
Q15796 (SMAD2) S255 Signor
Q15831 (STK11) S428 Signor
Q16204 (CCDC6) S244 HPRD
Q16254 (E2F4) S244 Calculation
Q16254 (E2F4) S384 Calculation
Q16559 (TAL2) S100 Calculation
Q16665 (HIF1A) S641 Signor
Q16665 (HIF1A) S643 Signor
Q16690 (DUSP5) T321 Calculation
Q16690 (DUSP5) S346 Calculation
Q16690 (DUSP5) S376 Calculation
Q16828 (DUSP6) S159 Signor
Q16828 (DUSP6) S174 Calculation
Q16828 (DUSP6) S197 Signor
Q16828 (DUSP6) S300 Calculation
Q2M1Z3 (ARHGAP31) T789 neXtProt
Q86UC2 (RSPH3) T286 Calculation
Q86UR1 (NOXA1) S282 Signor
Q86WB0 (ZC3HC1) S354 neXtProt
Q86WB0 (ZC3HC1) S359 neXtProt
Q8IX03 (WWC1) S542 Calculation
Q8IX03 (WWC1) S548 Signor
Q8IZP0 (ABI1) S183 Calculation
Q8IZP0 (ABI1) S216 Calculation
Q8IZP0 (ABI1) S225 Signor
Q8IZP0 (ABI1) T265 Calculation
Q8IZP0 (ABI1) S267 Calculation
Q8IZP0 (ABI1) S392 Calculation
Q8IZP0 (ABI1) T394 Calculation
Q8IZP0 (ABI1) S410 Calculation
Q8N122 (RPTOR) S8 Signor
Q8N122 (RPTOR) S696 Signor
Q8N122 (RPTOR) S863 Signor
Q8N9N5 (BANP) T337 Calculation
Q8N9N5 (BANP) T352 Calculation
Q8WX93 (PALLD) S688 Calculation
Q8WX93 (PALLD) S808 Calculation
Q92793 (CREBBP) S93 Calculation
Q92908 (GATA6) S266 Calculation
Q92934 (BAD) S75 Calculation
Q92974-2 (ARHGEF2) T678 Calculation
Q92974 (ARHGEF2) T679 Signor
Q92974-2 (ARHGEF2) S959 Calculation
Q93045-1 (STMN2) S62 HPRD, neXtProt
Q93045-1 (STMN2) S73 HPRD, neXtProt
Q969H0 (FBXW7) T205 Calculation
Q969V6 (MRTFA) S454 Signor
Q96FF9 (CDCA5) S79 Calculation
Q96FF9 (CDCA5) S209 Calculation
Q96Q27 (ASB2) S323 Calculation
Q96RK0 (CIC) S1389 Calculation
Q96RK0 (CIC) S1409 Signor
Q9BR01 (SULT4A1) T11 Signor
Q9BUB5 (MKNK1) T385 Signor
Q9BZI1 (IRX2) S317 neXtProt
Q9H8Y8 (GORASP2) T222 Calculation
Q9H8Y8 (GORASP2) T225 Calculation
Q9NQ66 (PLCB1) S982 Calculation
Q9NRA0 (SPHK2) S387 Signor
Q9NRA0 (SPHK2) T614 Signor
Q9NRF2 (SH2B1) S96 Calculation
Q9NYA1 (SPHK1) S225 Signor
Q9NYV6 (RRN3) S633 Signor
Q9NYV6 (RRN3) S649 Signor
Q9UBN7 (HDAC6) T1031 Signor
Q9UBN7 (HDAC6) S1035 Signor
Q9UHB6 (LIMA1) S362 Calculation
Q9UHB6 (LIMA1) S604 Calculation
Q9UIG0 (BAZ1B) S158 Signor
Q9UJY1 (HSPB8) S24 Calculation
Q9UJY1 (HSPB8) S27 Signor
Q9UJY1 (HSPB8) T87 Signor
Q9UJY1 (HSPB8) S159 neXtProt
Q9UKX7 (NUP50) S221 Signor
Q9UKX7 (NUP50) S315 Signor
Q9ULC4 (MCTS1) T81 Calculation
Q9UPT5 (EXOC7) S250 Calculation
Q9UQC2 (GAB2) S623 Signor
Q9Y6W5 (WASF2) S343 Calculation
Q9Y6W5 (WASF2) T346 Calculation
Q9Y6W5 (WASF2) S351 Calculation
Substrate Site Source
C9JLW8 (MCRIP1) S21 Calculation
C9JLW8 (MCRIP1) T30 Calculation
O00213 (APBB1) S175 Signor
O00213 (APBB1) S287 Signor
O00213 (APBB1) S347 Signor
O00213 (APBB1) T709 Signor
O00267 (SUPT5H) S666 neXtProt
O00429 (DNM1L) S616 Calculation
O00562 (PITPNM1) T794 Signor
O00562 (PITPNM1) T1223 Signor
O14613 (CDC42EP2) S101 neXtProt
O43251 (RBFOX2) T7 neXtProt
O43294 (TGFB1I1) S141 neXtProt
O43521 (BCL2L11) S69 Signor
O43524 (FOXO3) S294 Signor
O43524 (FOXO3) S344 Signor
O43524 (FOXO3) S425 Signor
O43623 (SNAI2) S87 Calculation
O43623 (SNAI2) S104 Calculation
O60331 (PIP5K1C) S650 neXtProt
O60502 (OGA) T709 neXtProt
O60504-2 (SORBS3) S188 Calculation
O60504 (SORBS3) S530 Signor
O60504 (SORBS3) T585 neXtProt
O60749 (SNX2) T104 neXtProt
O75030-9 (MITF) S73 Calculation
O75030 (MITF) S180 Signor
O75151 (PHF2) S655 neXtProt
O75581 (LRP6) S1490 Calculation
O75581 (LRP6) T1572 Calculation
O75582 (RPS6KA5) S360 Signor
O75582-1 (RPS6KA5) S376 HPRD
O75582 (RPS6KA5) T581 Signor
O75582 (RPS6KA5) T700 neXtProt
O94804 (STK10) T952 neXtProt
O94811 (TPPP) T14 neXtProt
O94811 (TPPP) S18 Calculation
O94811 (TPPP) S160 Calculation
O95785 (WIZ) S983 neXtProt
O95863 (SNAI1) S82 Calculation
O95863 (SNAI1) S104 Calculation
O95997 (PTTG1) S165 Signor
P00533 (EGFR) T693 Calculation
P01100 (FOS) T232 Signor
P01100 (FOS) T325 HPRD, neXtProt, Signor
P01100-1 (FOS) T331 HPRD, neXtProt, Signor
P01100 (FOS) S374 HPRD, neXtProt, Signor
P01106 (MYC) T58 Calculation
P01106 (MYC) S62 Calculation
P01106 (MYC) S71 neXtProt
P02545 (LMNA) S22 neXtProt
P02686-5 (MBP) T99 HPRD
P02686-1 (MBP) T229 HPRD, Signor
P02686 (MBP) T232 Signor
P03372 (ESR1) S104 Signor
P03372 (ESR1) S106 Signor
P03372 (ESR1) S118 Signor
P04049-1 (RAF1) S29 HPRD, neXtProt
P04049-1 (RAF1) S289 HPRD, neXtProt
P04049 (RAF1) S296 HPRD, neXtProt
P04049-1 (RAF1) S301 HPRD, neXtProt
P04049 (RAF1) S642 HPRD, neXtProt
P04150-2 (NR3C1) S226 HPRD, neXtProt
P04637-1 (TP53) S15 Calculation
P04637 (TP53) T55 Calculation
P05023 (ATP1A1) S16 neXtProt
P05198 (EIF2S1) S52 Calculation
P05787-1 (KRT8) S74 HPRD
P05787 (KRT8) T431 neXtProt
P06239 (LCK) S42 phospho.ELM, Signor
P06239 (LCK) S59 Signor
P06401 (PGR) S294 Signor
P07101-3 (TH) S31 Calculation
P07101-2 (TH) S58 Calculation
P07101 (TH) S62 Signor
P07196 (NEFL) T21 Calculation
P08047 (SP1) S59 Signor
P08047-1 (SP1) T453 Signor
P08047 (SP1) T739 Signor
P09874 (PARP1) S372 Signor
P09874 (PARP1) T373 Signor
P09917 (ALOX5) S664 Calculation
P10070 (GLI2) T903 neXtProt
P10242-1 (MYB) S532 HPRD
P10275-1 (AR) S516 Signor
P10415 (BCL2) T56 Signor
P10415 (BCL2) S70 neXtProt, Signor
P10415 (BCL2) T74 Signor
P10415-1 (BCL2) S87 Signor
P10636-8 (MAPT) S46 Calculation
P10636-8 (MAPT) T50 Calculation
P10636-6 (MAPT) T117 HPRD
P10636-2 (MAPT) T123 HPRD
P10636-2 (MAPT) S144 HPRD
P10636-8 (MAPT) T153 Calculation
P10636-8 (MAPT) T175 Calculation
P10636-8 (MAPT) T181 Calculation
P10636-8 (MAPT) S199 Calculation
P10636-8 (MAPT) S202 Calculation
P10636-8 (MAPT) T205 Calculation
P10636-8 (MAPT) T212 Calculation
P10636-8 (MAPT) T217 Calculation
P10636-8 (MAPT) T231 neXtProt
P10636-8 (MAPT) S235 Calculation
P10636-8 (MAPT) S396 Calculation
P10636-8 (MAPT) S404 Calculation
P10636-8 (MAPT) S422 Calculation
P10636 (MAPT) S519 Signor
P10636 (MAPT) T522 Signor
P10636 (MAPT) S721 Signor
P10646 (TFPI) S202 neXtProt
P10828 (THRB) S142 Signor
P11308 (ERG) S222 Calculation
P11308 (ERG) S283 Calculation
P11362 (FGFR1) S777 Signor
P11388 (TOP2A) S1213 Calculation
P11388 (TOP2A) S1247 Calculation
P11388 (TOP2A) S1354 Calculation
P11388 (TOP2A) S1361 Calculation
P11388 (TOP2A) S1393 Calculation
P12036 (NEFH) S518 HPRD
P12036 (NEFH) S526 HPRD
P12036 (NEFH) S532 HPRD
P12270 (TPR) T2116 neXtProt, Signor
P12270 (TPR) T2137 neXtProt, Signor
P12270 (TPR) S2155 neXtProt, Signor
P12270 (TPR) T2214 Signor
P12980 (LYL1) S36 Calculation
P14543 (NID1) S333 neXtProt
P14598 (NCF1) S345 Signor
P14598 (NCF1) S348 neXtProt
P14618 (PKM) S37 Calculation
P14635 (CCNB1) S126 Calculation
P14635 (CCNB1) S128 Calculation
P14784 (IL2RB) T476 Calculation
P14921 (ETS1) T38 Calculation
P15036 (ETS2) T72 neXtProt
P15056 (BRAF) S151 Calculation
P15056 (BRAF) T401 Calculation
P15056 (BRAF) S750 neXtProt
P15056 (BRAF) T753 Calculation
P15336 (ATF2) T69 Signor
P15336 (ATF2) T71 Signor
P15336 (ATF2) S90 Calculation
P15391-1 (CD19) Y348 HPRD
P15391-1 (CD19) Y378 HPRD
P15391-1 (CD19) Y409 HPRD
P15391-1 (CD19) Y439 HPRD
P15923-2 (TCF3) S352 Calculation
P15923-2 (TCF3) T355 Calculation
P15923-2 (TCF3) S359 Calculation
P16220 (CREB1) S133 Calculation
P16949 (STMN1) S25 neXtProt
P16949 (STMN1) S38 neXtProt, Signor
P17252 (PRKCA) T638 neXtProt
P17302 (GJA1) S255 HPRD, neXtProt, Signor
P17302 (GJA1) S279 Signor
P17302 (GJA1) S282 HPRD, Signor
P17480 (UBTF) T117 HPRD, neXtProt, phospho.ELM, Signor
P17480 (UBTF) T201 HPRD, neXtProt, phospho.ELM, Signor
P17535 (JUND) S100 Signor
P17655 (CAPN2) S50 Signor
P17676 (CEBPB) T235 Signor
P17861 (XBP1) S68 Calculation
P17861 (XBP1) S181 Calculation
P19419 (ELK1) S383 Calculation
P19419 (ELK1) S389 neXtProt
P19438-1 (TNFRSF1A) S274 HPRD
P19438-1 (TNFRSF1A) T280 HPRD
P19484 (TFEB) S142 Signor
P19525 (EIF2AK2) T451 Signor
P19634 (SLC9A1) S693 Calculation
P19634-1 (SLC9A1) S766 HPRD
P19634 (SLC9A1) S770 Signor
P19634-1 (SLC9A1) T779 HPRD
P19634-1 (SLC9A1) S785 Calculation
P19793 (RXRA) S260 Signor
P19878 (NCF2) T233 Calculation
P22736-1 (NR4A1) T143 HPRD
P22736 (NR4A1) S431 Calculation
P23396 (RPS3) T42 Signor
P23396 (RPS3) T221 neXtProt
P23443 (RPS6KB1) S434 Signor
P23443 (RPS6KB1) T444 Signor
P23443 (RPS6KB1) S447 Signor
P24046-1 (GABRR1) T394 HPRD
P24046-1 (GABRR1) S435 HPRD
P24046-1 (GABRR1) S440 HPRD
P24928 (POLR2A) S1619 phospho.ELM, Signor
P24928 (POLR2A) S1626 Signor
P24928 (POLR2A) S1647 Signor
P24928 (POLR2A) S1654 Signor
P24928 (POLR2A) S1668 Signor
P24928 (POLR2A) S1675 Signor
P24928 (POLR2A) S1696 Signor
P24928 (POLR2A) S1717 Signor
P24928 (POLR2A) S1724 Signor
P24928 (POLR2A) S1738 Signor
P24928 (POLR2A) S1766 Signor
P24928 (POLR2A) S1787 Signor
P24928 (POLR2A) S1864 Signor
P24928 (POLR2A) S1871 Signor
P24928 (POLR2A) S1878 Signor
P24928 (POLR2A) S1892 Signor
P24928 (POLR2A) S1899 Signor
P24928 (POLR2A) S1913 Signor
P24928 (POLR2A) S1920 Signor
P24928 (POLR2A) S1927 Signor
P24928 (POLR2A) S1934 Signor
P24928 (POLR2A) S1944 Signor
P24928 (POLR2A) S1951 Signor
P24941 (CDK2) T160 Signor
P27708 (CAD) T456 Signor
P28482-1 (MAPK1) S41 HPRD
P28482-1 (MAPK1) T185 HPRD
P28482 (MAPK1) Y187 HPRD, neXtProt
P28482 (MAPK1) T190 neXtProt, UniProt
P28482 (MAPK1) S246 Calculation
P28562 (DUSP1) S296 Signor
P28562 (DUSP1) S323 Signor
P28562 (DUSP1) S359 HPRD, Signor
P28562 (DUSP1) S364 Signor
P29590-4 (PML) T28 Signor
P29590 (PML) S36 Signor
P29590 (PML) S38 Signor
P29590 (PML) S40 Signor
P29590 (PML) S527 Signor
P29590-4 (PML) S530 Signor
P30041 (PRDX6) T177 Signor
P30307 (CDC25C) S216 Calculation
P31645 (SLC6A4) T616 Calculation
P31749 (AKT1) S473 Calculation
P31943 (HNRNPH1) S104 neXtProt
P32004 (L1CAM) S1204 Calculation
P32004 (L1CAM) S1248 Calculation
P32121 (ARRB2) S14 Calculation
P32121 (ARRB2) T276 Calculation
P33076 (CIITA) S280 Signor
P33076 (CIITA) S288 Signor
P33981 (TTK) S821 Calculation
P35236 (PTPN7) T66 Calculation
P35236 (PTPN7) S93 Calculation
P35236-2 (PTPN7) T105 HPRD
P35236-2 (PTPN7) S132 HPRD
P35398-4 (RORA) T128 Calculation
P35398 (RORA) T183 Signor
P35568 (IRS1) S312 Signor
P35568 (IRS1) S616 HPRD, neXtProt, Signor
P35568 (IRS1) S636 Calculation
P35568 (IRS1) S639 Calculation
P35658 (NUP214) S1710 Calculation
P35658 (NUP214) S1809 Calculation
P36956-3 (SREBF1) T81 Calculation
P36956-3 (SREBF1) S93 Calculation
P36956 (SREBF1) S117 Signor
P36956-4 (SREBF1) S147 HPRD
P37231-2 (PPARG) S84 HPRD
P37231 (PPARG) S112 Signor
P38936 (CDKN1A) T57 Signor
P38936 (CDKN1A) S130 Signor
P40763-2 (STAT3) S726 HPRD
P40763 (STAT3) S727 Calculation
P41162 (ETV3) S139 neXtProt
P41182 (BCL6) S333 Signor
P41182 (BCL6) S343 Signor
P42224 (STAT1) S727 neXtProt
P42229 (STAT5A) S780 Signor
P42702-1 (LIFR) S1044 Signor
P43354 (NR4A2) S126 phospho.ELM, Signor
P43354 (NR4A2) T132 phospho.ELM, Signor
P43694 (GATA4) S105 neXtProt
P46527 (CDKN1B) S10 Signor
P46527 (CDKN1B) S178 Signor
P46527 (CDKN1B) T187 Signor
P46695 (IER3) T18 Signor
P46821 (MAP1B) S1785 neXtProt
P47712 (PLA2G4A) S505 Signor
P48634 (PRRC2A) T610 neXtProt
P48634 (PRRC2A) S1004 neXtProt
P48634 (PRRC2A) S1219 neXtProt
P49023-2 (PXN) S83 Calculation
P49023-2 (PXN) S126 Calculation
P49023-2 (PXN) S130 Calculation
P49137 (MAPKAPK2) S9 Calculation
P49137 (MAPKAPK2) T25 Calculation
P49137 (MAPKAPK2) T222 Signor
P49137 (MAPKAPK2) S272 Signor
P49137 (MAPKAPK2) T334 Signor
P49137 (MAPKAPK2) T338 neXtProt
P49407-2 (ARRB1) S404 HPRD
P49407 (ARRB1) S412 Signor
P49418 (AMPH) S293 Signor
P49418 (AMPH) S295 Signor
P49585 (PCYT1A) S315 Signor
P49715 (CEBPA) S21 Signor
P49790 (NUP153) S257 Calculation
P49790 (NUP153) S320 Calculation
P49790 (NUP153) S334 Calculation
P49790 (NUP153) S338 Calculation
P49790 (NUP153) T369 Calculation
P49790 (NUP153) T388 Signor
P49790 (NUP153) T413 Calculation
P49790 (NUP153) S516 Calculation
P49790 (NUP153) S522 Calculation
P49790 (NUP153) S529 Signor
P49790 (NUP153) S614 neXtProt
P49795 (RGS19) S151 Signor
P49815-1 (TSC2) S540 HPRD
P49815-1 (TSC2) S664 Signor
P49841 (GSK3B) T43 Signor
P50548-1 (ERF) S161 Signor
P50548-1 (ERF) S246 Signor
P50548 (ERF) S251 Signor
P50548 (ERF) T526 Signor
P50616 (TOB1) S152 Signor
P50616 (TOB1) S154 Signor
P50616 (TOB1) S164 Signor
P51168 (SCNN1B) T615 neXtProt
P51170 (SCNN1G) T622 HPRD, neXtProt
P51812 (RPS6KA3) S386 neXtProt
P51812 (RPS6KA3) Y488 Calculation
P51812 (RPS6KA3) Y529 Calculation
P52945 (PDX1) S61 Calculation
P52945 (PDX1) S66 Calculation
P53355-1 (DAPK1) S734 Signor
P53805 (RCAN1) S167 neXtProt
P55211 (CASP9) T125 Signor
P61978 (HNRNPK) S284 Calculation
P68400 (CSNK2A1) T360 Signor
P68400 (CSNK2A1) S362 Signor
P68431 (HIST1H3J) S29 neXtProt
P78347-2 (GTF2I) S627 Calculation
P78347-2 (GTF2I) S633 Calculation
P78347 (GTF2I) S668 Signor
P78347 (GTF2I) S674 Signor
P78352 (DLG4) T287 neXtProt
P78536 (ADAM17) T735 Calculation
P78559 (MAP1A) T2042 neXtProt
P78559 (MAP1A) S2419 neXtProt
P84022 (SMAD3) T179 Signor
P84022 (SMAD3) S204 Signor
P84022 (SMAD3) S208 Signor
P84022-1 (SMAD3) S213 Signor
P84243 (H3F3B) S29 Signor
Q00587 (CDC42EP1) S113 neXtProt
Q00587 (CDC42EP1) S195 neXtProt
Q00613 (HSF1) S307 neXtProt
Q00975 (CACNA1B) S411 neXtProt
Q00975 (CACNA1B) S446 neXtProt
Q01196 (RUNX1) S249 Signor
Q01196 (RUNX1) S266 Signor
Q01196 (RUNX1) T273 Signor
Q01196-8 (RUNX1) S276 Signor
Q01196-8 (RUNX1) S293 Calculation
Q01196-8 (RUNX1) T300 Calculation
Q01196-8 (RUNX1) S303 Calculation
Q01196-8 (RUNX1) S462 Calculation
Q01844 (EWSR1) T79 Signor
Q01860 (POU5F1) S111 Signor
Q01860 (POU5F1) T118 Calculation
Q01860 (POU5F1) S355 Calculation
Q01959 (SLC6A3) S53 neXtProt
Q02224 (CENPE) S2605 Calculation
Q02224 (CENPE) S2608 Calculation
Q02224 (CENPE) S2639 Calculation
Q02224 (CENPE) S2654 Calculation
Q02447 (SP3) S73 Signor
Q02641 (CACNB1) S161 neXtProt
Q02641 (CACNB1) S348 neXtProt
Q02750 (MAP2K1) T286 Calculation
Q02750 (MAP2K1) T292 Calculation
Q02750 (MAP2K1) T386 Signor
Q02952 (AKAP12) S286 neXtProt
Q04206 (RELA) T435 phospho.ELM
Q04637-8 (EIF4G1) S1232 Calculation
Q05209 (PTPN12) S571 Calculation
Q05397 (PTK2) S910 Calculation
Q05469-1 (LIPE) T891 HPRD
Q05682-4 (CALD1) S534 Calculation
Q05682 (CALD1) S759 Signor
Q05682 (CALD1) S789 neXtProt, Signor
Q07343-2 (PDE4B) S487 Calculation
Q07343 (PDE4B) S659 Calculation
Q07666 (KHDRBS1) S58 HPRD, Signor
Q07666 (KHDRBS1) T72 Signor
Q07666-1 (KHDRBS1) T84 HPRD, Signor
Q07812 (BAX) T167 Calculation
Q07820 (MCL1) T163 Signor
Q07866 (KLC1) S460 neXtProt, Signor
Q07869-1 (PPARA) S12 HPRD, Signor
Q07869 (PPARA) S21 HPRD, Signor
Q07889 (SOS1) S1132 Calculation
Q07889-1 (SOS1) S1167 Calculation
Q07889-1 (SOS1) S1178 Calculation
Q07889 (SOS1) S1193 Calculation
Q07889-1 (SOS1) S1197 Calculation
Q08493-2 (PDE4C) S535 Calculation
Q08493 (PDE4C) S641 Signor
Q08499-5 (PDE4D) S413 Calculation
Q08499-4 (PDE4D) S490 Calculation
Q08499-2 (PDE4D) S579 Signor
Q08499 (PDE4D) S715 Signor
Q09472 (EP300) T317 Calculation
Q09472 (EP300) T938 Calculation
Q09472 (EP300) S1038 Calculation
Q09472 (EP300) T1960 Calculation
Q09472 (EP300) S2039 Calculation
Q09472 (EP300) S2279 Signor
Q09472 (EP300) S2315 Signor
Q09472 (EP300) S2366 Signor
Q09666 (AHNAK) S216 neXtProt
Q09666 (AHNAK) T694 neXtProt
Q09666 (AHNAK) S5099 neXtProt
Q09666 (AHNAK) S5110 neXtProt
Q09666 (AHNAK) T5794 neXtProt
Q12772 (SREBF2) S432 Signor
Q12772 (SREBF2) S455 Signor
Q12778 (FOXO1) S246 neXtProt
Q12778 (FOXO1) S413 neXtProt
Q12778 (FOXO1) S418 neXtProt
Q12778 (FOXO1) S429 neXtProt
Q12778 (FOXO1) S470 neXtProt
Q12778 (FOXO1) T478 neXtProt
Q12778 (FOXO1) T560 neXtProt
Q12800 (TFCP2) S291 Signor
Q12800 (TFCP2) S309 Signor
Q13153 (PAK1) T212 phospho.ELM
Q13164 (MAPK7) T733 Calculation
Q13285 (NR5A1) S203 Calculation
Q13322-3 (GRB10) S92 HPRD
Q13322 (GRB10) S150 Signor
Q13322-3 (GRB10) S360 HPRD
Q13322-2 (GRB10) S372 HPRD
Q13322 (GRB10) S418 HPRD, neXtProt
Q13322-2 (GRB10) S430 HPRD
Q13322 (GRB10) S476 Signor
Q13362 (PPP2R5C) S337 Signor
Q13409 (DYNC1I2) S87 neXtProt
Q13459 (MYO9B) T1346 neXtProt
Q13480-1 (GAB1) T312 HPRD, neXtProt
Q13480-2 (GAB1) S381 HPRD, neXtProt
Q13480 (GAB1) S454 HPRD, neXtProt
Q13480 (GAB1) T476 Calculation
Q13480-1 (GAB1) S551 HPRD
Q13480-1 (GAB1) S567 HPRD
Q13480-2 (GAB1) S581 HPRD
Q13480 (GAB1) S582 neXtProt
Q13480-2 (GAB1) S597 HPRD, neXtProt
Q13485 (SMAD4) T277 HPRD, Signor
Q13541 (EIF4EBP1) T37 Signor
Q13541 (EIF4EBP1) T45 Signor
Q13541 (EIF4EBP1) T46 Signor
Q13541 (EIF4EBP1) S65 Signor
Q13541 (EIF4EBP1) T70 Signor
Q13541 (EIF4EBP1) S83 HPRD, Signor
Q13562 (NEUROD1) S162 neXtProt
Q13562 (NEUROD1) S259 neXtProt
Q13562 (NEUROD1) S266 neXtProt
Q13562 (NEUROD1) S274 neXtProt
Q13586 (STIM1) S575 Signor
Q13586 (STIM1) S608 Calculation
Q13586 (STIM1) S621 Calculation
Q13614 (MTMR2) S58 Calculation
Q13625 (TP53BP2) T586 neXtProt
Q13625 (TP53BP2) S827 Calculation
Q14005 (IL16) S845 Signor
Q14151 (SAFB2) S343 neXtProt
Q14157-1 (UBAP2L) T844 neXtProt
Q14185 (DOCK1) T1772 neXtProt
Q14247 (CTTN) S405 Signor
Q14247 (CTTN) S418 Signor
Q14643 (ITPR1) S436 neXtProt
Q14643 (ITPR1) S1774 neXtProt
Q14654 (KCNJ11) T341 neXtProt
Q14654 (KCNJ11) S385 neXtProt
Q14674 (ESPL1) S1126 Signor
Q14686 (NCOA6) S884 Calculation
Q14790 (CASP8) S387 Signor
Q14934 (NFATC4) S676 Signor
Q15366 (PCBP2) S173 Calculation
Q15366 (PCBP2) S189 Calculation
Q15366 (PCBP2) T213 Calculation
Q15366 (PCBP2) S272 Calculation
Q15398 (DLGAP5) T329 neXtProt
Q15418-1 (RPS6KA1) T359 HPRD
Q15418-1 (RPS6KA1) S363 HPRD
Q15418-2 (RPS6KA1) T368 HPRD
Q15418-2 (RPS6KA1) S372 HPRD
Q15418 (RPS6KA1) T573 Signor
Q15596 (NCOA2) S736 Calculation
Q15648 (MED1) T1032 Signor
Q15648 (MED1) T1457 Signor
Q15672 (TWIST1) S6 neXtProt
Q15672 (TWIST1) S68 Signor
Q15700 (DLG2) S323 Calculation
Q15746 (MYLK) S1779 Calculation
Q15788-3 (NCOA1) S395 Signor
Q15788-2 (NCOA1) T1179 Signor
Q15788 (NCOA1) S1185 Signor
Q15796 (SMAD2) T220 Signor
Q15796 (SMAD2) S245 Signor
Q15796 (SMAD2) S250 Signor
Q15796 (SMAD2) S255 Signor
Q15797 (SMAD1) S187 Signor
Q15797 (SMAD1) S195 Signor
Q15797-1 (SMAD1) S206 Signor
Q15797 (SMAD1) S214 Signor
Q15831 (STK11) S428 Signor
Q16526 (CRY1) S247 neXtProt
Q16665 (HIF1A) S641 Signor
Q16665 (HIF1A) S643 Signor
Q16690 (DUSP5) T321 Calculation
Q16690 (DUSP5) S346 Calculation
Q16690 (DUSP5) S376 Calculation
Q16828 (DUSP6) S159 Signor
Q16828 (DUSP6) S197 Signor
Q49AN0 (CRY2) S266 neXtProt
Q53EZ4 (CEP55) S425 Signor
Q53EZ4 (CEP55) S428 Signor
Q5SW79 (CEP170) T565 neXtProt
Q5VZ89 (DENND4C) S989 neXtProt
Q6P0Q8 (MAST2) S1337 neXtProt
Q6P996 (PDXDC1) T691 neXtProt
Q6PKG0 (LARP1) T303 neXtProt
Q6PKG0 (LARP1) S774 neXtProt
Q6Y7W6 (GIGYF2) S30 neXtProt
Q6ZS17 (RIPOR1) S351 neXtProt
Q70E73 (RAPH1) S610 neXtProt
Q7L5N1 (COPS6) S148 Calculation
Q7LDG7 (RASGRP2) S394 Calculation
Q7Z5J4 (RAI1) T1068 neXtProt
Q86UC2 (RSPH3) T286 neXtProt
Q86UR1 (NOXA1) S239 neXtProt
Q86UR1 (NOXA1) S282 Signor
Q86UU1 (PHLDB1) S443 neXtProt
Q86UU1 (PHLDB1) S461 neXtProt
Q86WB0 (ZC3HC1) S354 neXtProt
Q86WB0 (ZC3HC1) S359 neXtProt
Q8IUW5 (RELL1) T261 neXtProt
Q8IUX7 (AEBP1) T621 neXtProt
Q8IUX7 (AEBP1) T1012 neXtProt
Q8IVF2 (AHNAK2) T510 neXtProt
Q8IVT5-4 (KSR1) T137 HPRD
Q8IVT5-4 (KSR1) T151 HPRD
Q8IVT5-4 (KSR1) S320 HPRD
Q8IW41 (MAPKAPK5) T182 Signor
Q8IX03 (WWC1) S542 Calculation
Q8IX03 (WWC1) S548 Signor
Q8IYB8 (SUPV3L1) S725 neXtProt
Q8IZP0 (ABI1) S183 Signor
Q8IZP0 (ABI1) S216 Calculation
Q8IZP0 (ABI1) S222 Signor
Q8IZP0 (ABI1) S225 Signor
Q8IZP0 (ABI1) T265 Calculation
Q8IZP0 (ABI1) S267 Calculation
Q8IZP0 (ABI1) S392 Calculation
Q8IZP0 (ABI1) T394 Calculation
Q8IZP0 (ABI1) S410 Calculation
Q8IZQ8 (MYOCD) S815 neXtProt
Q8IZQ8 (MYOCD) S862 neXtProt
Q8IZQ8 (MYOCD) S869 neXtProt
Q8IZQ8 (MYOCD) T896 neXtProt
Q8N122 (RPTOR) S8 Signor
Q8N122 (RPTOR) S696 Signor
Q8N122 (RPTOR) S863 Signor
Q8N1G1 (REXO1) T269 neXtProt
Q8N9N5 (BANP) T337 Calculation
Q8N9N5 (BANP) T352 Calculation
Q8NEL9 (DDHD1) S727 neXtProt
Q8NHW3 (MAFA) S14 Signor
Q8NHW3 (MAFA) S65 Signor
Q8TDM6 (DLG5) T1279 neXtProt
Q8TER0 (SNED1) T1239 neXtProt
Q8WX93 (PALLD) S688 Calculation
Q8WX93 (PALLD) S808 Calculation
Q92731 (ESR2) S105 neXtProt
Q92793 (CREBBP) S93 Calculation
Q92908 (GATA6) S266 Calculation
Q92934 (BAD) S75 Calculation
Q92974 (ARHGEF2) T679 Signor
Q93062 (RBPMS) T118 neXtProt
Q969H0 (FBXW7) T205 Calculation
Q969V6 (MRTFA) S454 Signor
Q96BY7 (ATG2B) T398 neXtProt
Q96EP5 (DAZAP1) T269 Calculation
Q96EP5 (DAZAP1) T315 Calculation
Q96FF9 (CDCA5) S79 Calculation
Q96FF9 (CDCA5) S209 Calculation
Q96I25 (RBM17) T71 Calculation
Q96I25 (RBM17) S222 Calculation
Q96LC9 (BMF) S74 Signor
Q96LC9 (BMF) S77 Signor
Q96LL9 (DNAJC30) S137 neXtProt
Q96PE2 (ARHGEF17) T702 neXtProt
Q96PH1-4 (NOX5) S498 Calculation
Q96PH1 (NOX5) S544 Signor
Q96Q27 (ASB2) S323 Calculation
Q96RK0 (CIC) S1389 Calculation
Q96RK0 (CIC) S1409 Signor
Q96RS0 (TGS1) S298 Calculation
Q96SB3 (PPP1R9B) S15 HPRD, Signor
Q9BQG0 (MYBBP1A) S1267 neXtProt
Q9BQQ3 (GORASP1) S274 neXtProt
Q9BUB5 (MKNK1) T255 Calculation
Q9BWW4 (SSBP3) T360 neXtProt
Q9BY84-1 (DUSP16) S446 Calculation
Q9BZI1 (IRX2) S46 neXtProt
Q9BZI1 (IRX2) S64 neXtProt
Q9C0C2 (TNKS1BP1) T131 neXtProt
Q9C0C2 (TNKS1BP1) T1032 neXtProt
Q9C0D5 (TANC1) S1564 neXtProt
Q9GZM8 (NDEL1) T219 Calculation
Q9GZM8 (NDEL1) T245 Calculation
Q9H0D6 (XRN2) T439 neXtProt
Q9H8Y8 (GORASP2) T222 Calculation
Q9H8Y8 (GORASP2) T225 Signor
Q9H9S0 (NANOG) S52 neXtProt
Q9H9S0 (NANOG) S71 neXtProt
Q9H9S0 (NANOG) T78 neXtProt
Q9HBH9 (MKNK2) S43 neXtProt
Q9HBH9 (MKNK2) S74 neXtProt
Q9HBH9 (MKNK2) S220 neXtProt
Q9HBH9 (MKNK2) T379 neXtProt
Q9HBH9 (MKNK2) S440 neXtProt
Q9HBH9 (MKNK2) S452 neXtProt
Q9NQ66 (PLCB1) S982 Calculation
Q9NQC3 (RTN4) T172 neXtProt
Q9NRA0 (SPHK2) S387 Signor
Q9NRA0 (SPHK2) T614 Signor
Q9NRA8 (EIF4ENIF1) S115 neXtProt
Q9NRF2 (SH2B1) S96 Calculation
Q9NSI2 (FAM207A) S39 neXtProt
Q9NUY8 (TBC1D23) T514 neXtProt
Q9NXR1 (NDE1) T215 HPRD, Signor
Q9NXR1 (NDE1) S239 HPRD, Signor
Q9NYA1 (SPHK1) S225 Signor
Q9NYZ3 (GTSE1) S461 neXtProt
Q9NZ72 (STMN3) S68 Calculation
Q9NZV8 (KCND2) T602 neXtProt
Q9NZV8 (KCND2) T607 neXtProt
Q9NZV8 (KCND2) S616 neXtProt
Q9P0K7 (RAI14) T249 neXtProt
Q9UBN7 (HDAC6) T1031 Calculation
Q9UHB6 (LIMA1) S362 Calculation
Q9UHB6 (LIMA1) S604 Calculation
Q9UKX7 (NUP50) S221 Signor
Q9UKX7 (NUP50) S315 Signor
Q9ULC4 (MCTS1) T81 Calculation
Q9ULW0 (TPX2) T369 neXtProt
Q9UNA1 (ARHGAP26) S685 neXtProt
Q9UPT5 (EXOC7) S250 Signor
Q9UQC2 (GAB2) S623 Signor
Q9Y463 (DYRK1B) S421 Calculation
Q9Y4F5 (CEP170B) S969 neXtProt
Q9Y4H2 (IRS2) T657 neXtProt
Q9Y4K1 (CRYBG1) S675 neXtProt
Q9Y698 (CACNG2) T321 neXtProt
Q9Y6G9 (DYNC1LI1) S516 neXtProt
Q9Y6W5 (WASF2) S296 Calculation
Q9Y6W5 (WASF2) S308 Calculation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.