Product: S-Tag Antibody
Catalog: T0016
Description: Mouse monoclonal antibody to S-Tag
Application: WB ELISA
Reactivity: All
Uniprot:
RRID: AB_2834421

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Product Info

Source:
Mouse
Application:
WB 1:3000-1:10000, ELISA 1:3000-1:5000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
All
Clonality:
Monoclonal [T198]
Specificity:
S- Tag antibody can recognize C-terminal, internal, and N-terminal S-tagged proteins.
RRID:
AB_2834421
Cite Format: Affinity Biosciences Cat# T0016, RRID:AB_2834421.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

A synthetic peptide KETAAAKFERQHMDS coupled to KLH.

Description:
S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). If RNase A is digested with subtilisin, a single peptide bond is cleaved, but the resulting two products remain weakly bound to each other and the protein, called ribonuclease S, remains active although each of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag.The amino acid sequence of the S-tag is: KETAAAKFERQHMDS conjugated to KLH. S- Tag antibody can recognize C-terminal, internal, and N-terminal S-tagged proteins.

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