Product: DIRA1 Antibody
Catalog: AF0516
Description: Rabbit polyclonal antibody to DIRA1
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 22kDa; 22kD(Calculated).
Uniprot: O95057
RRID: AB_2834402

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(82%), Bovine(82%), Horse(82%), Sheep(82%), Dog(82%)
Clonality:
Polyclonal
Specificity:
DIRA1 Antibody detects endogenous levels of total DIRA1.
RRID:
AB_2834402
Cite Format: Affinity Biosciences Cat# AF0516, RRID:AB_2834402.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Di Ras1; DIRAS family GTP binding RAS like 1; Distinct subgroup of the Ras family member 1; FLJ42681; GBTS1; GTP binding protein Di Ras1; Ras related inhibitor of cell growth; RIG; Rig protein; Small GTP binding tumor suppressor 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O95057 DIRA1_HUMAN:

Highly expressed in heart and brain.

Description:
DIRAS1 Displays low GTPase activity and exist predominantly in the GTP-bound form. Belongs to the small GTPase superfamily. Di-Ras family. Note: This description may include information from UniProtKB.
Sequence:
MPEQSNDYRVVVFGAGGVGKSSLVLRFVKGTFRDTYIPTIEDTYRQVISCDKSVCTLQITDTTGSHQFPAMQRLSISKGHAFILVFSVTSKQSLEELGPIYKLIVQIKGSVEDIPVMLVGNKCDETQREVDTREAQAVAQEWKCAFMETSAKMNYNVKELFQELLTLETRRNMSLNIDGKRSGKQKRTDRVKGKCTLM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
82
Horse
82
Bovine
82
Sheep
82
Dog
82
Chicken
73
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95057 As Substrate

Site PTM Type Enzyme
K29 Ubiquitination
S53 Phosphorylation
K180 Ubiquitination

Research Backgrounds

Function:

Displays low GTPase activity and exists predominantly in the GTP-bound form.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in heart and brain.

Family&Domains:

Belongs to the small GTPase superfamily. Di-Ras family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.