Product: Phospho-RSK1 p90 (Ser380) Antibody
Catalog: DF7702
Source: Rabbit
Application: WB, IF/ICC, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 90 kD; 83kD(Calculated).
Uniprot: Q15418
RRID: AB_2841173

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-RSK1 p90 (Ser380) Antibody detects endogenous levels of RSK1 p90 only when phosphorylated at S380.
RRID:
AB_2841173
Cite Format: Affinity Biosciences Cat# DF7702, RRID:AB_2841173.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1; dJ590P13.1; EC 2.7.11.1; HU 1; HU1; KS6A1_HUMAN; MAP kinase activated protein kinase 1a; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; MAPKAPK1A; MGC79981; Mitogen-activated protein kinase-activated protein kinase 1A; OTTHUMP00000004113; p90 RSK1; p90-RSK 1; p90rsk; p90RSK1; p90S6K; pp90RSK1; Ribosomal protein S6 kinase 90kD 1; Ribosomal protein S6 kinase 90kD polypeptide 1; Ribosomal protein S6 kinase 90kDa polypeptide 1; Ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase alpha-1; Ribosomal protein S6 kinase polypeptide 1; Ribosomal S6 kinase 1; RPS6K1 alpha; rps6ka; Rps6ka1; RSK 1; RSK 1 p90; RSK; RSK-1; RSK1; S6K alpha 1; S6K-alpha-1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15418 As Substrate

Site PTM Type Enzyme
S45 Phosphorylation
T47 Phosphorylation
K51 Ubiquitination
S54 Phosphorylation
K56 Ubiquitination
K66 Ubiquitination
S72 Phosphorylation
K75 Acetylation
K75 Ubiquitination
K81 Ubiquitination
Y91 Phosphorylation
K94 Ubiquitination
Y138 Phosphorylation
S154 Phosphorylation
K189 Ubiquitination
S209 Phosphorylation
K210 Ubiquitination
Y220 Phosphorylation
S221 Phosphorylation Q15418 (RPS6KA1) , O15530 (PDPK1)
T225 Phosphorylation
Y228 Phosphorylation
K278 Ubiquitination
S307 Phosphorylation
K316 Ubiquitination
T359 Phosphorylation
S363 Phosphorylation
S369 Phosphorylation
S380 Phosphorylation P49137 (MAPKAPK2) , P41279 (MAP3K8) , Q13237 (PRKG2) , Q15418 (RPS6KA1)
T384 Phosphorylation
K392 Ubiquitination
S402 Phosphorylation
K410 Ubiquitination
K421 Ubiquitination
K433 Ubiquitination
K438 Ubiquitination
K453 Ubiquitination
Y466 Phosphorylation
T474 Phosphorylation
K476 Ubiquitination
Y486 Phosphorylation
T489 Phosphorylation
K500 Ubiquitination
K505 Ubiquitination
S513 Phosphorylation
T522 Phosphorylation
K537 Ubiquitination
K562 Ubiquitination
T573 Phosphorylation P28482 (MAPK1)
Y576 Phosphorylation
T577 Phosphorylation
K587 Ubiquitination
K633 Ubiquitination
S637 Phosphorylation
K649 Ubiquitination
K654 Ubiquitination
K667 Ubiquitination
K678 Ubiquitination
K680 Ubiquitination
S684 Phosphorylation
S687 Phosphorylation
T701 Phosphorylation
Y702 Phosphorylation
S703 Phosphorylation
K709 Ubiquitination
S732 Phosphorylation
T733 Phosphorylation
T734 Phosphorylation

PTMs - Q15418 As Enzyme

Substrate Site Source
O00418 (EEF2K) S366 Uniprot
O14727 (APAF1) S268 Uniprot
O14727 (APAF1) S357 Uniprot
O14757 (CHEK1) S280 Uniprot
O60343 (TBC1D4) S341 Uniprot
O60343 (TBC1D4) S751 Uniprot
O75030-9 (MITF) S409 Uniprot
P01100 (FOS) S362 Uniprot
P03372-1 (ESR1) S118 Uniprot
P03372-1 (ESR1) S167 Uniprot
P11831 (SRF) S103 Uniprot
P13796 (LCP1) S5 Uniprot
P16144 (ITGB4) S1364 Uniprot
P16220-1 (CREB1) S133 Uniprot
P17676 (CEBPB) T235 Uniprot
P17676-1 (CEBPB) T266 Uniprot
P19634 (SLC9A1) S703 Uniprot
P21333 (FLNA) S2152 Uniprot
P22736-1 (NR4A1) S351 Uniprot
P23588 (EIF4B) S422 Uniprot
P25963 (NFKBIA) S32 Uniprot
P25963 (NFKBIA) S36 Uniprot
P29317 (EPHA2) S897 Uniprot
P30304 (CDC25A) S293 Uniprot
P30304 (CDC25A) S295 Uniprot
P30305 (CDC25B) S353 Uniprot
P30305 (CDC25B) T355 Uniprot
P30307 (CDC25C) S247 Uniprot
P32004-1 (L1CAM) S1152 Uniprot
P42345 (MTOR) T2446 Uniprot
P42345 (MTOR) S2448 Uniprot
P43354-1 (NR4A2) S347 Uniprot
P46527 (CDKN1B) T198 Uniprot
P49427 (CDC34) T162 Uniprot
P49815 (TSC2) S1798 Uniprot
P49841 (GSK3B) S9 Uniprot
P50549 (ETV1) S191 Uniprot
P50549 (ETV1) S216 Uniprot
P50549-1 (ETV1) S334 Uniprot
P50552 (VASP) T278 Uniprot
P53355 (DAPK1) S289 Uniprot
P60983 (GMFB) T27 Uniprot
P62753 (RPS6) S235 Uniprot
P62753 (RPS6) S236 Uniprot
P62753 (RPS6) S240 Uniprot
P62753 (RPS6) S244 Uniprot
P67809 (YBX1) S102 Uniprot
P78371 (CCT2) S260 Uniprot
P84243 (H3F3B) S11 Uniprot
Q04206 (RELA) S536 Uniprot
Q05195 (MXD1) S145 Uniprot
Q07889 (SOS1) S1134 Uniprot
Q07889 (SOS1) S1161 Uniprot
Q13164 (MAPK7) S496 Uniprot
Q15418 (RPS6KA1) S221 Uniprot
Q15418-1 (RPS6KA1) T225 Uniprot
Q15418-2 (RPS6KA1) T234 Uniprot
Q15418-1 (RPS6KA1) S380 Uniprot
Q15418-2 (RPS6KA1) S389 Uniprot
Q15418-1 (RPS6KA1) T577 Uniprot
Q15418-2 (RPS6KA1) T586 Uniprot
Q15653 (NFKBIB) S19 Uniprot
Q15653 (NFKBIB) S23 Uniprot
Q15831 (STK11) S428 Uniprot
Q16821-1 (PPP1R3A) S46 Uniprot
Q16875 (PFKFB3) S461 Uniprot
Q8IX03 (WWC1) T929 Uniprot
Q8IX03 (WWC1) S947 Uniprot
Q8N122 (RPTOR) S719 Uniprot
Q8N122 (RPTOR) S721 Uniprot
Q8N122 (RPTOR) S722 Uniprot
Q8TB45 (DEPTOR) S286 Uniprot
Q8TB45 (DEPTOR) S287 Uniprot
Q8TB45 (DEPTOR) S291 Uniprot
Q92570 (NR4A3) S376 Uniprot
Q92570-3 (NR4A3) S387 Uniprot
Q92882 (OSTF1) S202 Uniprot
Q92934 (BAD) S75 Uniprot
Q92934 (BAD) S99 Uniprot
Q92934 (BAD) S118 Uniprot
Q92934 (BAD) S153 Uniprot
Q96RK0 (CIC) S173 Uniprot
Q9H6Z4 (RANBP3) S126 Uniprot
Q9HC62 (SENP2) T369 Uniprot
Q9UBP6-1 (METTL1) S27 Uniprot
Q9UN36 (NDRG2) S332 Uniprot
Q9UN36 (NDRG2) S350 Uniprot
Q9Y2V2 (CARHSP1) S52 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB (By similarity). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration.

PTMs:

Activated by phosphorylation at Ser-221 by PDPK1. Autophosphorylated on Ser-380, as part of the activation process. May be phosphorylated at Thr-359 and Ser-363 by MAPK1/ERK2 and MAPK3/ERK1.

N-terminal myristoylation results in an activated kinase in the absence of added growth factors.

Subcellular Location:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation. Interacts with ETV1/ER81 and FGFR1.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.