FOXJ3 Antibody - #AF0602
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Protocols
Product Info
Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
FOXJ3 Antibody detects endogenous levels of total FOXJ3.
RRID:
AB_2834393
Cite Format: Affinity Biosciences Cat# AF0602, RRID:AB_2834393.
Cite Format: Affinity Biosciences Cat# AF0602, RRID:AB_2834393.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Forkhead box J3; Forkhead box protein J3; FOXJ3; FOXJ3_HUMAN; KIAA1041; MGC165036; MGC176686;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Description:
FOXJ3 2 isoforms of the human protein are produced by alternative splicing.
Sequence:
- Q9UPW0 FOXJ3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGLYGQACPSVTSLRMTSELESSLTSMDWLPQLTMRAAIQKSDATQNAHGTGISKKNALLDPNTTLDQEEVQQHKDGKPPYSYASLITFAINSSPKKKMTLSEIYQWICDNFPYYREAGSGWKNSIRHNLSLNKCFLKVPRSKDDPGKGSYWAIDTNPKEDVLPTRPKKRARSVERASTPYSIDSDSLGMECIISGSASPTLAINTVTNKVTLYNTDQDGSDSPRSSLNNSLSDQSLASVNLNSVGSVHSYTPVTSHPESVSQSLTPQQQPQYNLPERDKQLLFSEYNFEDLSASFRSLYKSVFEQSLSQQGLMNIPSESSQQSHTSCTYQHSPSSTVSTHPHSNQSSLSNSHGSGLNTTGSNSVAQVSLSHPQMHTQPSPHPPHRPHGLPQHPQRSPHPAPHPQQHSQLQSPHPQHPSPHQHIQHHPNHQHQTLTHQAPPPPQQVSCNSGVSNDWYATLDMLKESCRIASSVNWSDVDLSQFQGLMESMRQADLKNWSLDQVQFADLCSSLNQFFTQTGLIHSQSNVQQNVCHGAMHPTKPSQHIGTGNLYIDSRQNLPPSVMPPPGYPHIPQALSTPGTTMAGHHRAMNQQHMMPSQAFQMRRSLPPDDIQDDFDWDSIV
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Bovine
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UPW0 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S22 | Phosphorylation | Uniprot | |
| K41 | Sumoylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| T45 | Phosphorylation | Uniprot | |
| T51 | Phosphorylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| T64 | Phosphorylation | Uniprot | |
| T65 | Phosphorylation | Uniprot | |
| Y81 | Phosphorylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| Y214 | Phosphorylation | Uniprot | |
| T216 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| S472 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| S606 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Transcriptional activator of MEF2C involved in the regulation pf adult muscle fiber type identity and skeletal muscle regeneration.
Subcellular Location:
Nucleus.
References
1). Multiple roles of FOXJ3 in spermatogenesis: A lesson from Foxj3 conditional knockout mouse models. MOLECULAR REPRODUCTION AND DEVELOPMENT, 2016
(PubMed: 27739607)
[IF=2.5]
Application: WB Species: mouse Sample:
Figure 1. Loss of FOXJ3 in different germ cell population. A: FOXJ3 protein abundance in testes at Embryonic Day 15.5 (E15.5), Postnatal Day 3 (PND3), and 1, 2, and 3 weeks old (W). GAPDH served as a loading control. B: FOXJ3 immunostaining of adult mouse testes. Scale bars, 100 μm. C: Strategy for Mvh- or Stra8-driven Cre recombinase-induced deletion of Foxj3 (exon 3) in male germ cells. Black, numbered boxes represent exons in the Foxj3 gene. D: FOXJ3 immunoblot for Mvh-cKO and control testes at PND3. E: FOXJ3 immunoblot for Stra8-cKO and control spermatocyte lysate. GAPDH served as a loading control.
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