Product: Phospho-MAPKAPK2 (Thr334) Antibody
Catalog: DF7632
Source: Rabbit
Application: WB, IF/ICC, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Mol.Wt.: 46 kD.; 46kD(Calculated).
Uniprot: P49137
RRID: AB_2841117

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-MAPKAPK2 (Thr334) Antibody detects endogenous levels of MAPKAPK2 only when phosphorylated at threonine 334 (human) and threonine 320 (mouse and rat).
RRID:
AB_2841117
Cite Format: Affinity Biosciences Cat# DF7632, RRID:AB_2841117.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MAP kinase activated protein Kinase 2; MAP kinase-activated protein kinase 2; MAPK activated protein kinase 2; MAPK-activated protein kinase 2; MAPK2_HUMAN; MAPKAP K2; MAPKAP kinase 2; MAPKAPK 2; MAPKAPK-2; MAPKAPK2; Mitogen activated protein kinase activated protein kinase 2; MK 2; MK2;

Immunogens

Immunogen:

Synthetic phosphopeptide from around the phosphorylation site of threonine 334 (PQTPPL) of human MAPKAP Kinase 2.

Uniprot:
Gene(ID):
Expression:
P49137 MAPK2_HUMAN:

Expressed in all tissues examined.

Sequence:
MLSNSQGQSPPVPFPAPAPPPQPPTPALPHPPAQPPPPPPQQFPQFHVKSGLQIKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHNSLTTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWEDVKEEMTSALATMRVDYEQIKIKKIEDASNPLLLKRRKKARALEAAALAH

PTMs - P49137 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation P28482 (MAPK1)
T25 Phosphorylation Q16539 (MAPK14) , P28482 (MAPK1)
K56 Ubiquitination
Y63 Phosphorylation
T66 Phosphorylation
S67 Phosphorylation
K84 Ubiquitination
K93 Ubiquitination
K100 Ubiquitination
K188 Ubiquitination
K204 Ubiquitination
T206 Phosphorylation
K212 Ubiquitination
T214 Phosphorylation
T215 Phosphorylation
S219 Phosphorylation
T221 Phosphorylation
T222 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3) , Q16539 (MAPK14)
Y225 Phosphorylation
T226 Phosphorylation
S272 Phosphorylation P28482 (MAPK1) , Q16539 (MAPK14)
K307 Ubiquitination
T317 Phosphorylation
S328 Phosphorylation
T329 Phosphorylation
T334 Phosphorylation Q16539 (MAPK14) , P28482 (MAPK1) , P27361 (MAPK3)
T338 Phosphorylation P28482 (MAPK1) , P49137 (MAPKAPK2)
S339 Phosphorylation
K353 Sumoylation
K353 Ubiquitination
Y367 Phosphorylation
K371 Ubiquitination
K374 Ubiquitination
K385 Ubiquitination

PTMs - P49137 As Enzyme

Substrate Site Source
O00418 (EEF2K) S377 Uniprot
O15511 (ARPC5) S77 Uniprot
O75925 (PIAS1) S522 Uniprot
O95453 (PARN) S557 Uniprot
O95816 (BAG2) S20 Uniprot
P04637 (TP53) S20 Uniprot
P04792 (HSPB1) S15 Uniprot
P04792 (HSPB1) S78 Uniprot
P04792 (HSPB1) S82 Uniprot
P05783 (KRT18) S53 Uniprot
P07101-2 (TH) S19 Uniprot
P07101 (TH) S40 Uniprot
P07101-4 (TH) S44 Uniprot
P07101-2 (TH) S67 Uniprot
P07101 (TH) S71 Uniprot
P07101-3 (TH) S404 Uniprot
P08670 (VIM) S39 Uniprot
P08670 (VIM) S51 Uniprot
P08670 (VIM) S56 Uniprot
P08670 (VIM) S83 Uniprot
P09917 (ALOX5) S272 Uniprot
P11831 (SRF) S85 Uniprot
P11831 (SRF) S103 Uniprot
P16220-1 (CREB1) S133 Uniprot
P17844 (DDX5) S198 Uniprot
P26651 (ZFP36) S60 Uniprot
P26651 (ZFP36) S66 Uniprot
P26651 (ZFP36) S113 Uniprot
P26651 (ZFP36) S184 Uniprot
P26651 (ZFP36) S186 Uniprot
P26651 (ZFP36) T257 Uniprot
P26651 (ZFP36) T271 Uniprot
P26651 (ZFP36) S273 Uniprot
P26651 (ZFP36) S323 Uniprot
P27815 (PDE4A) S152 Uniprot
P30305 (CDC25B) S169 Uniprot
P30305 (CDC25B) S323 Uniprot
P30305 (CDC25B) S353 Uniprot
P30305 (CDC25B) S375 Uniprot
P30307 (CDC25C) S216 Uniprot
P31749 (AKT1) S473 Uniprot
P33241-2 (LSP1) S142 Uniprot
P33241-2 (LSP1) S190 Uniprot
P33241-1 (LSP1) S204 Uniprot
P33241 (LSP1) S252 Uniprot
P35900 (KRT20) S13 Uniprot
P49137 (MAPKAPK2) T338 Uniprot
P49815 (TSC2) S1254 Uniprot
P50549-1 (ETV1) S191 Uniprot
P50549-1 (ETV1) S216 Uniprot
P51812 (RPS6KA3) S386 Uniprot
P53350 (PLK1) S326 Uniprot
P53350 (PLK1) S383 Uniprot
P53667 (LIMK1) S323 Uniprot
P63104 (YWHAZ) S58 Uniprot
Q00613 (HSF1) S121 Uniprot
Q00987 (MDM2) S157 Uniprot
Q00987 (MDM2) S166 Uniprot
Q01094 (E2F1) S364 Uniprot
Q07352 (ZFP36L1) S54 Uniprot
Q07352 (ZFP36L1) S92 Uniprot
Q07352 (ZFP36L1) S203 Uniprot
Q07817 (BCL2L1) S62 Uniprot
Q13151 (HNRNPA0) S84 Uniprot
Q13263 (TRIM28) S473 Uniprot
Q14134 (TRIM29) S550 Uniprot
Q14134 (TRIM29) S552 Uniprot
Q14457 (BECN1) S90 Uniprot
Q15418 (RPS6KA1) S380 Uniprot
Q16875 (PFKFB3) S461 Uniprot
Q6JBY9 (RCSD1) S179 Uniprot
Q6JBY9 (RCSD1) S244 Uniprot
Q7L5N7 (LPCAT2) S34 Uniprot
Q969V6 (MRTFA) S312 Uniprot
Q969V6 (MRTFA) S333 Uniprot
Q96J92 (WNK4) S575 Uniprot
Q9H1R3 (MYLK2) S151 Uniprot
Q9NQC3-2 (RTN4) S7 Uniprot
Q9NQC3-2 (RTN4) S107 Uniprot
Q9NY61 (AATF) T366 Uniprot
Q9UKV8 (AGO2) S387 Uniprot
Q9UPN4 (CEP131) S47 Uniprot
Q9UPN4 (CEP131) S78 Uniprot
Q9Y385 (UBE2J1) S184 Uniprot

Research Backgrounds

Function:

Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites. Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.

PTMs:

Sumoylation inhibits the protein kinase activity.

Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-222, Ser-272 and Thr-334.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Phosphorylation and subsequent activation releases the autoinhibitory helix, resulting in the export from the nucleus into the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues examined.

Subunit Structure:

Heterodimer with p38-alpha/MAPK14; this heterodimer forms a stable complex: molecules are positioned 'face to face' so that the ATP-binding sites of both kinases are at the heterodimer interface. Interacts with PHC2. Interacts with HSF1.

Family&Domains:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Research Fields

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

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