Product: Bamacan Antibody
Catalog: DF7558
Description: Rabbit polyclonal antibody to Bamacan
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 142kDa; 142kD(Calculated).
Uniprot: Q9UQE7
RRID: AB_2841052

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(86%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Bamacan Antibody detects endogenous levels of total Bamacan.
RRID:
AB_2841052
Cite Format: Affinity Biosciences Cat# DF7558, RRID:AB_2841052.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BAM; Bamacan; Basement membrane associated chondroitin proteoglycan; Basement membrane-associated chondroitin proteoglycan; BMH; CDLS3; chondroitin sulfate proteoglycan 6 (bamacan); Chondroitin sulfate proteoglycan 6; Chromosome associated polypeptide; Chromosome-associated polypeptide; CSPG 6; CSPG6; hCAP; Human chromosome associated polypeptide; im:7142991; SMC 3; SMC protein 3; SMC-3; smc3; SMC3_HUMAN; SMC3L1; Structural maintenance of chromosome 3; Structural maintenance of chromosomes 3; Structural maintenance of chromosomes protein 3; u:fb22e01; wu:fc30d07;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MYIKQVIIQGFRSYRDQTIVDPFSSKHNVIVGRNGSGKSNFFYAIQFVLSDEFSHLRPEQRLALLHEGTGPRVISAFVEIIFDNSDNRLPIDKEEVSLRRVIGAKKDQYFLDKKMVTKNDVMNLLESAGFSRSNPYYIVKQGKINQMATAPDSQRLKLLREVAGTRVYDERKEESISLMKETEGKREKINELLKYIEERLHTLEEEKEELAQYQKWDKMRRALEYTIYNQELNETRAKLDELSAKRETSGEKSRQLRDAQQDARDKMEDIERQVRELKTKISAMKEEKEQLSAERQEQIKQRTKLELKAKDLQDELAGNSEQRKRLLKERQKLLEKIEEKQKELAETEPKFNSVKEKEERGIARLAQATQERTDLYAKQGRGSQFTSKEERDKWIKKELKSLDQAINDKKRQIAAIHKDLEDTEANKEKNLEQYNKLDQDLNEVKARVEELDRKYYEVKNKKDELQSERNYLWREENAEQQALAAKREDLEKKQQLLRAATGKAILNGIDSINKVLDHFRRKGINQHVQNGYHGIVMNNFECEPAFYTCVEVTAGNRLFYHIVDSDEVSTKILMEFNKMNLPGEVTFLPLNKLDVRDTAYPETNDAIPMISKLRYNPRFDKAFKHVFGKTLICRSMEVSTQLARAFTMDCITLEGDQVSHRGALTGGYYDTRKSRLELQKDVRKAEEELGELEAKLNENLRRNIERINNEIDQLMNQMQQIETQQRKFKASRDSILSEMKMLKEKRQQSEKTFMPKQRSLQSLEASLHAMESTRESLKAELGTDLLSQLSLEDQKRVDALNDEIRQLQQENRQLLNERIKLEGIITRVETYLNENLRKRLDQVEQELNELRETEGGTVLTATTSELEAINKRVKDTMARSEDLDNSIDKTEAGIKELQKSMERWKNMEKEHMDAINHDTKELEKMTNRQGMLLKKKEECMKKIRELGSLPQEAFEKYQTLSLKQLFRKLEQCNTELKKYSHVNKKALDQFVNFSEQKEKLIKRQEELDRGYKSIMELMNVLELRKYEAIQLTFKQVSKNFSEVFQKLVPGGKATLVMKKGDVEGSQSQDEGEGSGESERGSGSQSSVPSVDQFTGVGIRVSFTGKQGEMREMQQLSGGQKSLVALALIFAIQKCDPAPFYLFDEIDQALDAQHRKAVSDMIMELAVHAQFITTTFRPELLESADKFYGVKFRNKVSHIDVITAEMAKDFVEDDTTHG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
86
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UQE7 As Substrate

Site PTM Type Enzyme
T18 Phosphorylation
K26 Ubiquitination
T69 Phosphorylation
R72 Methylation
S85 Phosphorylation
K105 Acetylation
K106 Acetylation
K106 Ubiquitination
Y109 Phosphorylation
K113 Sumoylation
K113 Ubiquitination
K118 Ubiquitination
Y136 Phosphorylation
K140 Acetylation
K140 Ubiquitination
K143 Ubiquitination
R155 Methylation
K172 Ubiquitination
S175 Phosphorylation
S177 Phosphorylation
K180 Ubiquitination
K188 Ubiquitination
K194 Ubiquitination
K207 Ubiquitination
Y213 Phosphorylation
K215 Acetylation
K215 Ubiquitination
Y225 Phosphorylation
R236 Methylation
K238 Ubiquitination
S243 Phosphorylation
K245 Methylation
K245 Ubiquitination
R272 Methylation
S292 Phosphorylation
R323 Methylation
K336 Acetylation
K350 Ubiquitination
K378 Ubiquitination
K409 Methylation
K427 Methylation
K429 Methylation
K436 Ubiquitination
K445 Methylation
K445 Ubiquitination
R453 Methylation
K459 Ubiquitination
K486 Methylation
K486 Ubiquitination
K503 Ubiquitination
Y560 Phosphorylation
S565 Phosphorylation
K592 Ubiquitination
Y600 Phosphorylation
K612 Ubiquitination
Y615 Phosphorylation
S635 Phosphorylation
S639 Phosphorylation
R644 Methylation
Y668 Phosphorylation
Y669 Phosphorylation
S674 Phosphorylation
K695 Ubiquitination
S731 Phosphorylation
S737 Phosphorylation
T773 Phosphorylation
T783 Phosphorylation
S787 Phosphorylation
S790 Phosphorylation
K795 Acetylation
K795 Ubiquitination
Y831 Phosphorylation
R851 Methylation
K871 Ubiquitination
S886 Phosphorylation
K889 Ubiquitination
T926 Phosphorylation
K956 Acetylation
K956 Ubiquitination
K963 Ubiquitination
K968 Acetylation
K968 Ubiquitination
K977 Ubiquitination
K985 Ubiquitination
K997 Methylation
K997 Ubiquitination
K1002 Ubiquitination
R1009 Methylation
K1012 Ubiquitination
S1013 Phosphorylation
K1025 Ubiquitination
K1034 Ubiquitination
K1038 Ubiquitination
K1046 Ubiquitination
K1059 Ubiquitination
S1065 Phosphorylation
S1067 Phosphorylation P19784 (CSNK2A2) , P68400 (CSNK2A1)
S1074 Phosphorylation
S1077 Phosphorylation
R1079 Methylation
S1081 Phosphorylation
S1083 Phosphorylation Q13315 (ATM)
S1085 Phosphorylation
S1086 Phosphorylation
K1105 Ubiquitination
S1116 Phosphorylation
K1190 Acetylation
K1190 Ubiquitination
K1194 Ubiquitination
K1207 Ubiquitination

Research Backgrounds

Function:

Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.

PTMs:

Phosphorylated at Ser-1083 in a SPO11-dependent manner.

Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication. Deacetylation by HDAC8, regulates release of the cohesin complex from chromatin.

Subcellular Location:

Nucleus. Chromosome. Chromosome>Centromere.
Note: Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. The phosphorylated form at Ser-1083 is preferentially associated with unsynapsed chromosomal regions (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms a heterodimer with SMC1A or SMC1B in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their SMC hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Also found in meiosis-specific cohesin complexes. Found in a complex with SMC1A, CDCA5 and RAD21, PDS5A/SCC-112 and PDS5B/APRIN. Interacts with NUMA1, and forms a ternary complex with KIF3B and KIFAP3, suggesting a function in tethering the chromosomes to the spindle pole and in chromosome movement. Interacts with PDS5A and WAPL; regulated by SMC3 acetylation. Interacts (via SMC hinge domain) with KIAA1328 (via N- and C-terminal domains). Interacts with DDX11. Found in a cohesin complex with SMC1A, STAG1 and RAD21. The SMC1A-SMC3 heterodimer interacts with the NIPBL-MAU2 heterodimer. Interacts with MXI1, MXD3, MXD4, SYCP2, RPGR and STAG3 (By similarity).

Family&Domains:

The flexible SMC hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC1A or SMC1B, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure (By similarity).

Belongs to the SMC family. SMC3 subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

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