Product: Phospho-Cdk1/2 (Thr14) Antibody
Catalog: DF2944
Source: Rabbit
Application: WB, IHC, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 34kD; 34kD(Calculated).
Uniprot: P06493 | P24941
RRID: AB_2840928

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-Cdk1/2 (Thr14) Antibody detects endogenous levels of Cdk1/2 only when phosphorylated at Thr14.
RRID:
AB_2840928
Cite Format: Affinity Biosciences Cat# DF2944, RRID:AB_2840928.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cdc 2; Cdc2; CDC28A; CDK 1; CDK1; CDK1_HUMAN; CDKN1; CELL CYCLE CONTROLLER CDC2; Cell division control protein 2; Cell division control protein 2 homolog; Cell division cycle 2 G1 to S and G2 to M; Cell division protein kinase 1; Cell Divsion Cycle 2 Protein; Cyclin Dependent Kinase 1; Cyclin-dependent kinase 1; DKFZp686L20222; MGC111195; p34 Cdk1; p34 protein kinase; P34CDC2; Cdc2 related protein kinase; cdc2-related protein kinase; CDC28; CDC2A; Cdk 2; CDK1; CDK2; CDK2_HUMAN; CDKN2; Cell devision kinase 2; Cell division protein kinase 2; Cyclin dependent kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; kinase Cdc2; MPF; p33 protein kinase; p33(CDK2);

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P06493 CDK1_HUMAN:

Isoform 2 is found in breast cancer tissues.

Sequence:
MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM

MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P06493/P24941 As Substrate

Site PTM Type Enzyme
M1 Acetylation
Y4 Phosphorylation P19525 (EIF2AK2)
T5 Phosphorylation
K6 Acetylation
K6 Sumoylation
K6 Ubiquitination
K9 Acetylation
K9 Sumoylation
K9 Ubiquitination
T14 Phosphorylation Q99640 (PKMYT1)
Y15 Phosphorylation P12931 (SRC) , P30291 (WEE1) , Q99640 (PKMYT1) , P04626 (ERBB2) , P07948 (LYN)
Y19 Phosphorylation
K20 Ubiquitination
K24 Sumoylation
K24 Ubiquitination
K33 Acetylation
K33 Sumoylation
K33 Ubiquitination
K34 Acetylation
K34 Sumoylation
K34 Ubiquitination
S39 Phosphorylation P68400 (CSNK2A1)
S46 Phosphorylation
S53 Phosphorylation
K56 Ubiquitination
Y77 Phosphorylation
K89 Ubiquitination
C119 S-Nitrosylation
S121 Phosphorylation
K130 Sumoylation
K130 Ubiquitination
K139 Sumoylation
K139 Ubiquitination
T141 Phosphorylation
K143 Ubiquitination
Y160 Phosphorylation
T161 Phosphorylation P41279 (MAP3K8) , P50613 (CDK7)
S171 Phosphorylation
S178 Phosphorylation
K201 Sumoylation
K201 Ubiquitination
S208 Phosphorylation O14757 (CHEK1)
T222 Phosphorylation
S233 Phosphorylation
K238 Sumoylation
K238 Ubiquitination
T240 Phosphorylation
K243 Ubiquitination
K245 Sumoylation
K245 Ubiquitination
S248 Phosphorylation
K254 Sumoylation
K254 Ubiquitination
S265 Phosphorylation
K266 Ubiquitination
Y270 Phosphorylation
K274 Ubiquitination
S277 Phosphorylation
K279 Sumoylation
K279 Ubiquitination
Y286 Phosphorylation
K295 Sumoylation
K295 Ubiquitination
K296 Ubiquitination
Site PTM Type Enzyme
M1 Acetylation
K6 Acetylation
K6 Ubiquitination
K9 Ubiquitination
T14 Phosphorylation P30291 (WEE1)
Y15 Phosphorylation P07948 (LYN) , P30291 (WEE1)
Y19 Phosphorylation
K20 Ubiquitination
K24 Ubiquitination
K33 Sumoylation
K33 Ubiquitination
T39 Phosphorylation P31749 (AKT1)
S46 Phosphorylation P50613 (CDK7)
T47 Phosphorylation
K56 Ubiquitination
K65 Ubiquitination
K129 Ubiquitination
T137 Phosphorylation
K142 Ubiquitination
T158 Phosphorylation
Y159 Phosphorylation
T160 Phosphorylation P24941 (CDK2) , P28482 (MAPK1) , Q8IZL9 (CDK20) , P50613 (CDK7) , P27361 (MAPK3)
T165 Phosphorylation P50613 (CDK7)
Y168 Phosphorylation P50613 (CDK7)
K237 Ubiquitination
K250 Ubiquitination
K273 Ubiquitination
K278 Ubiquitination
K291 Ubiquitination
R297 Methylation

PTMs - P06493/P24941 As Enzyme

Substrate Site Source
A6NKT7 (RGPD3) S1276 Uniprot
O00267 (SUPT5H) S666 Uniprot
O00311 (CDC7) T376 Uniprot
O00399 (DCTN6) T186 Uniprot
O00418 (EEF2K) S359 Uniprot
O00429 (DNM1L) S616 Uniprot
O00443 (PIK3C2A) S259 Uniprot
O00562 (PITPNM1) T287 Uniprot
O00562 (PITPNM1) S382 Uniprot
O00571 (DDX3X) T204 Uniprot
O00571 (DDX3X) T323 Uniprot
O14715 (RGPD8) S1275 Uniprot
O14745 (SLC9A3R1) S280 Uniprot
O14745 (SLC9A3R1) S302 Uniprot
O14757 (CHEK1) S286 Uniprot
O14757 (CHEK1) S301 Uniprot
O14773 (TPP1) T201 Uniprot
O14974 (PPP1R12A) S432 Uniprot
O14974 (PPP1R12A) S473 Uniprot
O14974 (PPP1R12A) S601 Uniprot
O14980 (XPO1) S391 Uniprot
O14994 (SYN3) S470 Uniprot
O15151 (MDM4) S96 Uniprot
O15287 (FANCG) S383 Uniprot
O15287 (FANCG) S387 Uniprot
O15287 (FANCG) T487 Uniprot
O15350-1 (TP73) T86 Uniprot
O15392 (BIRC5) T34 Uniprot
O15446 (CD3EAP) S285 Uniprot
O43521-2 (BCL2L11) S44 Uniprot
O43663 (PRC1) T481 Uniprot
O43683 (BUB1) S593 Uniprot
O43683 (BUB1) T609 Uniprot
O60331 (PIP5K1C) S650 Uniprot
O60566 (BUB1B) S543 Uniprot
O60566 (BUB1B) T620 Uniprot
O60566 (BUB1B) S670 Uniprot
O60566 (BUB1B) S1043 Uniprot
O60610 (DIAPH1) T768 Uniprot
O60701 (UGDH) T461 Uniprot
O75122 (CLASP2) S507 Uniprot
O75122 (CLASP2) S541 Uniprot
O75152 (ZC3H11A) T321 Uniprot
O75154 (RAB11FIP3) S102 Uniprot
O94782 (USP1) S313 Uniprot
O94916 (NFAT5) T135 Uniprot
O95251 (KAT7) T85 Uniprot
O95251 (KAT7) T88 Uniprot
O95835-1 (LATS1) T490 Uniprot
O95835 (LATS1) S613 Uniprot
O95997 (PTTG1) S165 Uniprot
P00338 (LDHA) T248 Uniprot
P00519-1 (ABL1) S569 Uniprot
P00519-2 (ABL1) S588 Uniprot
P00533 (EGFR) S1026 Uniprot
P02545-3 (LMNA) T19 Uniprot
P02545-1 (LMNA) S22 Uniprot
P02545 (LMNA) S390 Uniprot
P02545 (LMNA) S392 Uniprot
P04179 (SOD2) S106 Uniprot
P04183 (TK1) S13 Uniprot
P04637 (TP53) S315 Uniprot
P05067 (APP) T743 Uniprot
P05412 (JUN) S63 Uniprot
P05412 (JUN) S73 Uniprot
P05412 (JUN) S243 Uniprot
P05783 (KRT18) S34 Uniprot
P05787 (KRT8) S432 Uniprot
P06400 (RB1) S249 Uniprot
P06400 (RB1) T252 Uniprot
P06400 (RB1) T373 Uniprot
P06400 (RB1) S807 Uniprot
P06400 (RB1) S811 Uniprot
P06493-2 (CDK1) Y19 Uniprot
P06748 (NPM1) S70 Uniprot
P06748 (NPM1) T199 Uniprot
P06748-2 (NPM1) T205 Uniprot
P06748-2 (NPM1) T208 Uniprot
P06748-1 (NPM1) T219 Uniprot
P06748 (NPM1) T234 Uniprot
P06748 (NPM1) T237 Uniprot
P08047 (SP1) T739 Uniprot
P08670 (VIM) S55 Uniprot
P08670 (VIM) S56 Uniprot
P0C1Z6 (TFPT) S180 Uniprot
P10275 (AR) S83 Uniprot
P10275 (AR) S310 Uniprot
P10275 (AR) S516 Uniprot
P10412 (HIST1H1E) T18 Uniprot
P10415 (BCL2) T56 Uniprot
P10415 (BCL2) S70 Uniprot
P10636-6 (MAPT) T95 Uniprot
P10636-8 (MAPT) T153 Uniprot
P10636-8 (MAPT) S202 Uniprot
P10636-8 (MAPT) T205 Uniprot
P10636-8 (MAPT) T212 Uniprot
P10636-8 (MAPT) T231 Uniprot
P10636-8 (MAPT) S235 Uniprot
P10636-8 (MAPT) S404 Uniprot
P11171-2 (EPB41) T60 Uniprot
P11171-2 (EPB41) S679 Uniprot
P11387 (TOP1) S112 Uniprot
P11387 (TOP1) S394 Uniprot
P11388 (TOP2A) S1213 Uniprot
P11388 (TOP2A) S1247 Uniprot
P11388 (TOP2A) S1354 Uniprot
P11388 (TOP2A) S1361 Uniprot
P11388 (TOP2A) S1393 Uniprot
P12270 (TPR) S2073 Uniprot
P12272 (PTHLH) T108 Uniprot
P12272 (PTHLH) T121 Uniprot
P13051 (UNG) S64 Uniprot
P14136-1 (GFAP) S8 Uniprot
P14635 (CCNB1) S126 Uniprot
P14635 (CCNB1) S128 Uniprot
P15172 (MYOD1) S5 Uniprot
P15172 (MYOD1) S200 Uniprot
P15531 (NME1) T94 Uniprot
P15531 (NME1) S120 Uniprot
P15927 (RPA2) S23 Uniprot
P15927 (RPA2) S29 Uniprot
P15927 (RPA2) S33 Uniprot
P16220 (CREB1) S270 Uniprot
P16220 (CREB1) S271 Uniprot
P16949 (STMN1) S25 Uniprot
P16949-1 (STMN1) S38 Uniprot
P17096 (HMGA1) S36 Uniprot
P17096-2 (HMGA1) T42 Uniprot
P17096 (HMGA1) T53 Uniprot
P17096-2 (HMGA1) T67 Uniprot
P17096 (HMGA1) T78 Uniprot
P17302 (GJA1) S255 Uniprot
P17661 (DES) S32 Uniprot
P17676 (CEBPB) S76 Uniprot
P17706-2 (PTPN2) S304 Uniprot
P18031 (PTPN1) S386 Uniprot
P18754-1 (RCC1) S2 Uniprot
P18754 (RCC1) S11 Uniprot
P18754 (RCC1) T274 Uniprot
P18754 (RCC1) S387 Uniprot
P18858 (LIG1) S76 Uniprot
P19338 (NCL) T121 Uniprot
P19338 (NCL) T641 Uniprot
P19338 (NCL) T707 Uniprot
P20338 (RAB4A) S204 Uniprot
P20700 (LMNB1) S23 Uniprot
P20700 (LMNB1) S393 Uniprot
P21333 (FLNA) S1084 Uniprot
P21333 (FLNA) S1436 Uniprot
P21333 (FLNA) S1459 Uniprot
P21333 (FLNA) S1533 Uniprot
P21333 (FLNA) S1630 Uniprot
P22234 (PAICS) S27 Uniprot
P22314-1 (UBA1) S4 Uniprot
P22314-1 (UBA1) S835 Uniprot
P22392 (NME2) T94 Uniprot
P23396 (RPS3) T221 Uniprot
P23443 (RPS6KB1) S394 Uniprot
P23443 (RPS6KB1) S434 Uniprot
P23443 (RPS6KB1) T444 Uniprot
P23443 (RPS6KB1) S447 Uniprot
P23769 (GATA2) T176 Uniprot
P24928 (POLR2A) S1920 Uniprot
P24928 (POLR2A) S1934 Uniprot
P25205 (MCM3) S112 Uniprot
P26358 (DNMT1) S154 Uniprot
P26368 (U2AF2) S79 Uniprot
P27361-3 (MAPK3) S343 Uniprot
P27816 (MAP4) S696 Uniprot
P27816 (MAP4) S787 Uniprot
P27816 (MAP4) T892 Uniprot
P27816 (MAP4) T901 Uniprot
P27816 (MAP4) T917 Uniprot
P27824 (CANX) S583 Uniprot
P29590 (PML) S518 Uniprot
P29692 (EEF1D) S133 Uniprot
P30101 (PDIA3) S456 Uniprot
P30260 (CDC27) T205 Uniprot
P30260 (CDC27) T209 Uniprot
P30260 (CDC27) S222 Uniprot
P30260 (CDC27) T289 Uniprot
P30260 (CDC27) S312 Uniprot
P30260 (CDC27) T329 Uniprot
P30260 (CDC27) T343 Uniprot
P30260 (CDC27) S364 Uniprot
P30260 (CDC27) S426 Uniprot
P30260 (CDC27) T430 Uniprot
P30260 (CDC27) S434 Uniprot
P30260 (CDC27) S435 Uniprot
P30260 (CDC27) T446 Uniprot
P30291 (WEE1) S123 Uniprot
P30304 (CDC25A) S18 Uniprot
P30304 (CDC25A) S116 Uniprot
P30305-2 (CDC25B) S146 Uniprot
P30305 (CDC25B) S160 Uniprot
P30305-2 (CDC25B) S307 Uniprot
P30305 (CDC25B) S321 Uniprot
P30307 (CDC25C) T48 Uniprot
P30307 (CDC25C) T67 Uniprot
P30307-1 (CDC25C) S122 Uniprot
P30307 (CDC25C) T130 Uniprot
P30307 (CDC25C) S168 Uniprot
P30307 (CDC25C) S214 Uniprot
P31350-1 (RRM2) S20 Uniprot
P31350 (RRM2) T33 Uniprot
P31948 (STIP1) T198 Uniprot
P31948 (STIP1) T332 Uniprot
P33316-2 (DUT) S11 Uniprot
P33316 (DUT) S99 Uniprot
P33991 (MCM4) T7 Uniprot
P33991 (MCM4) T19 Uniprot
P33991 (MCM4) S32 Uniprot
P33991 (MCM4) S88 Uniprot
P33991 (MCM4) T110 Uniprot
P33993 (MCM7) S121 Uniprot
P35251 (RFC1) T506 Uniprot
P35251 (RFC1) S518 Uniprot
P35611 (ADD1) S12 Uniprot
P35611 (ADD1) S355 Uniprot
P36956 (SREBF1) S439 Uniprot
P38398 (BRCA1) S1189 Uniprot
P38398 (BRCA1) S1191 Uniprot
P38398 (BRCA1) S1497 Uniprot
P39748 (FEN1) S187 Uniprot
P39880-1 (CUX1) S1237 Uniprot
P39880 (CUX1) S1270 Uniprot
P40763 (STAT3) S727 Uniprot
P42166 (TMPO) S424 Uniprot
P43307 (SSR1) S268 Uniprot
P46013 (MKI67) T761 Uniprot
P46013 (MKI67) T1943 Uniprot
P46060 (RANGAP1) T409 Uniprot
P46060 (RANGAP1) S428 Uniprot
P46060 (RANGAP1) S442 Uniprot
P46937 (YAP1) T119 Uniprot
P46937 (YAP1) S289 Uniprot
P46937 (YAP1) S367 Uniprot
P47736-1 (RAP1GAP) S484 Uniprot
P48200 (IREB2) S157 Uniprot
P48681 (NES) T315 Uniprot
P49418-1 (AMPH) S272 Uniprot
P49418-1 (AMPH) S276 Uniprot
P49418 (AMPH) S285 Uniprot
P49450 (CENPA) S68 Uniprot
P49459-2 (UBE2A) S90 Uniprot
P49459 (UBE2A) S120 Uniprot
P49715 (CEBPA) S21 Uniprot
P49715 (CEBPA) S234 Uniprot
P49792 (RANBP2) T2153 Uniprot
P49792 (RANBP2) S2246 Uniprot
P49792 (RANBP2) S2251 Uniprot
P49792 (RANBP2) S2276 Uniprot
P49792 (RANBP2) S2280 Uniprot
P49915 (GMPS) T318 Uniprot
P49916 (LIG3) T191 Uniprot
P49916 (LIG3) S210 Uniprot
P49916 (LIG3) S913 Uniprot
P50570 (DNM2) S764 Uniprot
P50570 (DNM2) T766 Uniprot
P50613 (CDK7) S164 Uniprot
P50613 (CDK7) T170 Uniprot
P51587 (BRCA2) S3291 Uniprot
P52732 (KIF11) T926 Uniprot
P52926 (HMGA2) S44 Uniprot
P52926 (HMGA2) S59 Uniprot
P52948-2 (NUP98) T529 Uniprot
P52948-2 (NUP98) T536 Uniprot
P52948-2 (NUP98) S595 Uniprot
P52948-2 (NUP98) S606 Uniprot
P52948 (NUP98) S612 Uniprot
P52948 (NUP98) S623 Uniprot
P52948-2 (NUP98) T653 Uniprot
P52948 (NUP98) T670 Uniprot
P54132 (BLM) S714 Uniprot
P54132 (BLM) T766 Uniprot
P54274 (TERF1) T344 Uniprot
P54274 (TERF1) T371 Uniprot
P54578 (USP14) T235 Uniprot
P55211 (CASP9) T125 Uniprot
P61020-1 (RAB5B) S123 Uniprot
P61978 (HNRNPK) S216 Uniprot
P62136 (PPP1CA) T320 Uniprot
P63279 (UBE2I) S71 Uniprot
P67870 (CSNK2B) S209 Uniprot
P68400-2 (CSNK2A1) T208 Uniprot
P68400-2 (CSNK2A1) T224 Uniprot
P68400-2 (CSNK2A1) S226 Uniprot
P68400-2 (CSNK2A1) S234 Uniprot
P68400 (CSNK2A1) T344 Uniprot
P68400 (CSNK2A1) T360 Uniprot
P68400 (CSNK2A1) S362 Uniprot
P68400 (CSNK2A1) S370 Uniprot
P82970 (HMGN5) T31 Uniprot
Q01094 (E2F1) S332 Uniprot
Q01094 (E2F1) S337 Uniprot
Q01167 (FOXK2) S373 Uniprot
Q01167 (FOXK2) S428 Uniprot
Q01196 (RUNX1) S21 Uniprot
Q01196 (RUNX1) S249 Uniprot
Q01196 (RUNX1) S266 Uniprot
Q01196 (RUNX1) T273 Uniprot
Q01196-8 (RUNX1) S276 Uniprot
Q01196-8 (RUNX1) S293 Uniprot
Q01196-8 (RUNX1) T300 Uniprot
Q01196-8 (RUNX1) S303 Uniprot
Q01196 (RUNX1) S397 Uniprot
Q01196-8 (RUNX1) S424 Uniprot
Q01581 (HMGCS1) S495 Uniprot
Q02539 (HIST1H1A) T4 Uniprot
Q02750 (MAP2K1) T286 Uniprot
Q02750 (MAP2K1) T292 Uniprot
Q02880 (TOP2B) S1424 Uniprot
Q02952 (AKAP12) T767 Uniprot
Q03060-16 (CREM) S87 Uniprot
Q03060-16 (CREM) S110 Uniprot
Q03060-6 (CREM) S136 Uniprot
Q04637 (EIF4G1) S1231 Uniprot
Q05682-5 (CALD1) T377 Uniprot
Q05682-4 (CALD1) T383 Uniprot
Q05682-3 (CALD1) T403 Uniprot
Q05682-6 (CALD1) T409 Uniprot
Q05682-5 (CALD1) S463 Uniprot
Q05682-4 (CALD1) S469 Uniprot
Q05682-5 (CALD1) T469 Uniprot
Q05682-4 (CALD1) T475 Uniprot
Q05682-5 (CALD1) S483 Uniprot
Q05682-3 (CALD1) S489 Uniprot
Q05682-6 (CALD1) S494 Uniprot
Q05682-3 (CALD1) T495 Uniprot
Q05682-5 (CALD1) S498 Uniprot
Q05682-6 (CALD1) T500 Uniprot
Q05682-4 (CALD1) S504 Uniprot
Q05682-3 (CALD1) S509 Uniprot
Q05682-6 (CALD1) S514 Uniprot
Q05682-3 (CALD1) S524 Uniprot
Q05682-6 (CALD1) S529 Uniprot
Q05682-1 (CALD1) T638 Uniprot
Q05682-1 (CALD1) S724 Uniprot
Q05682-1 (CALD1) T730 Uniprot
Q05682-1 (CALD1) S744 Uniprot
Q05682-1 (CALD1) S759 Uniprot
Q06413 (MEF2C) S396 Uniprot
Q06481 (APLP2) T736 Uniprot
Q06830 (PRDX1) T90 Uniprot
Q07666 (KHDRBS1) T317 Uniprot
Q07820 (MCL1) S64 Uniprot
Q07820 (MCL1) T92 Uniprot
Q08050 (FOXM1) S251 Uniprot
Q08050-2 (FOXM1) T596 Uniprot
Q08050 (FOXM1) T600 Uniprot
Q08050-1 (FOXM1) T611 Uniprot
Q08050 (FOXM1) T620 Uniprot
Q08050-3 (FOXM1) T649 Uniprot
Q08379 (GOLGA2) S37 Uniprot
Q09472 (EP300) S1038 Uniprot
Q09472 (EP300) S2039 Uniprot
Q09666 (AHNAK) S5763 Uniprot
Q12778 (FOXO1) S249 Uniprot
Q12888 (TP53BP1) T1609 Uniprot
Q12888 (TP53BP1) S1678 Uniprot
Q12959 (DLG1) S158 Uniprot
Q12959 (DLG1) S443 Uniprot
Q13042 (CDC16) S112 Uniprot
Q13042 (CDC16) S560 Uniprot
Q13042 (CDC16) T581 Uniprot
Q13188 (STK3) S385 Uniprot
Q13415 (ORC1) S258 Uniprot
Q13415 (ORC1) S273 Uniprot
Q13415 (ORC1) T375 Uniprot
Q13428 (TCOF1) S156 Uniprot
Q13428 (TCOF1) S583 Uniprot
Q13428 (TCOF1) T983 Uniprot
Q13501 (SQSTM1) T269 Uniprot
Q13501 (SQSTM1) S272 Uniprot
Q13535 (ATR) S428 Uniprot
Q13541 (EIF4EBP1) T37 Uniprot
Q13541 (EIF4EBP1) T46 Uniprot
Q13541 (EIF4EBP1) S65 Uniprot
Q13541 (EIF4EBP1) T70 Uniprot
Q13541 (EIF4EBP1) S83 Uniprot
Q13586 (STIM1) S668 Uniprot
Q13950 (RUNX2) S465 Uniprot
Q14005-3 (IL16) T56 Uniprot
Q14005-1 (IL16) T757 Uniprot
Q14157 (UBAP2L) S454 Uniprot
Q14202 (ZMYM3) T826 Uniprot
Q14203 (DCTN1) S212 Uniprot
Q14244 (MAP7) S209 Uniprot
Q14247 (CTTN) S405 Uniprot
Q14493 (SLBP) T62 Uniprot
Q14643 (ITPR1) S421 Uniprot
Q14643 (ITPR1) T800 Uniprot
Q14674 (ESPL1) S1126 Uniprot
Q14739 (LBR) S71 Uniprot
Q14739 (LBR) S86 Uniprot
Q14790 (CASP8) S387 Uniprot
Q14807 (KIF22) S427 Uniprot
Q14807 (KIF22) T463 Uniprot
Q14978 (NOLC1) T607 Uniprot
Q14978 (NOLC1) T610 Uniprot
Q14980 (NUMA1) T2015 Uniprot
Q14980 (NUMA1) T2055 Uniprot
Q14980 (NUMA1) S2087 Uniprot
Q14980 (NUMA1) T2106 Uniprot
Q14C86 (GAPVD1) S950 Uniprot
Q14CS0 (UBXN2B) S56 Uniprot
Q14CS0 (UBXN2B) T59 Uniprot
Q15149-7 (PLEC) T4370 Uniprot
Q15149-8 (PLEC) T4380 Uniprot
Q15149-9 (PLEC) T4388 Uniprot
Q15149-5 (PLEC) T4402 Uniprot
Q15149-6 (PLEC) T4406 Uniprot
Q15149 (PLEC) T4539 Uniprot
Q15398 (DLGAP5) T329 Uniprot
Q15398 (DLGAP5) S618 Uniprot
Q15398 (DLGAP5) T639 Uniprot
Q15398 (DLGAP5) S839 Uniprot
Q15717 (ELAVL1) S202 Uniprot
Q15910 (EZH2) T345 Uniprot
Q15910 (EZH2) T487 Uniprot
Q16513 (PKN2) S535 Uniprot
Q16659 (MAPK6) S684 Uniprot
Q16659 (MAPK6) S688 Uniprot
Q16659 (MAPK6) T698 Uniprot
Q16659 (MAPK6) S705 Uniprot
Q16665 (HIF1A) S668 Uniprot
Q2M2I8 (AAK1) T389 Uniprot
Q2NKX8 (ERCC6L) T1063 Uniprot
Q38SD2 (LRRK1) T1427 Uniprot
Q53EZ4 (CEP55) S425 Uniprot
Q53EZ4 (CEP55) S428 Uniprot
Q5BJF6-3 (ODF2) S796 Uniprot
Q5FBB7 (SGO1) T346 Uniprot
Q6PGQ7 (BORA) S252 Uniprot
Q6Y7W6-3 (GIGYF2) S30 Uniprot
Q7Z3J3 (RGPD4) S1276 Uniprot
Q86WB0 (ZC3HC1) S395 Uniprot
Q8IWB6 (TEX14) T618 Uniprot
Q8IWB6 (TEX14) T727 Uniprot
Q8IWB6 (TEX14) T728 Uniprot
Q8IX03 (WWC1) S542 Uniprot
Q8IX03 (WWC1) S931 Uniprot
Q8IXJ6 (SIRT2) S368 Uniprot
Q8IZD2 (KMT2E) T912 Uniprot
Q8IZP0 (ABI1) S216 Uniprot
Q8N122 (RPTOR) S696 Uniprot
Q8N122 (RPTOR) T706 Uniprot
Q8NEB9 (PIK3C3) T159 Uniprot
Q8NFH8-2 (REPS2) S411 Uniprot
Q8NFH8 (REPS2) S463 Uniprot
Q8NHV4 (NEDD1) T550 Uniprot
Q8TAP9 (MPLKIP) S93 Uniprot
Q8TAP9 (MPLKIP) S104 Uniprot
Q8TAP9 (MPLKIP) T120 Uniprot
Q8TEM1-1 (NUP210) S1881 Uniprot
Q8TF76 (HASPIN) T128 Uniprot
Q8WWK9 (CKAP2) T623 Uniprot
Q8WX93 (PALLD) S641 Uniprot
Q8WX93 (PALLD) S766 Uniprot
Q92574 (TSC1) T417 Uniprot
Q92574-1 (TSC1) S584 Uniprot
Q92574 (TSC1) T1047 Uniprot
Q92934 (BAD) S91 Uniprot
Q92993 (KAT5) S86 Uniprot
Q92993 (KAT5) S90 Uniprot
Q92993-3 (KAT5) S119 Uniprot
Q92993-3 (KAT5) S123 Uniprot
Q93045-1 (STMN2) S73 Uniprot
Q96C19 (EFHD2) S74 Uniprot
Q96C36 (PYCR2) S304 Uniprot
Q96EB6 (SIRT1) T530 Uniprot
Q96EB6 (SIRT1) S540 Uniprot
Q96FF9 (CDCA5) S21 Uniprot
Q96FF9 (CDCA5) S33 Uniprot
Q96FF9 (CDCA5) T48 Uniprot
Q96FF9 (CDCA5) S75 Uniprot
Q96FF9 (CDCA5) S79 Uniprot
Q96FF9 (CDCA5) S83 Uniprot
Q96FF9 (CDCA5) T111 Uniprot
Q96FF9 (CDCA5) T115 Uniprot
Q96FF9 (CDCA5) S126 Uniprot
Q96FF9 (CDCA5) S139 Uniprot
Q96FF9 (CDCA5) T151 Uniprot
Q96FF9 (CDCA5) T159 Uniprot
Q96FF9 (CDCA5) S164 Uniprot
Q96FF9 (CDCA5) S181 Uniprot
Q96FF9 (CDCA5) S209 Uniprot
Q96JH7 (VCPIP1) T761 Uniprot
Q96JH7 (VCPIP1) S768 Uniprot
Q96KB5 (PBK) T9 Uniprot
Q96Q89-3 (KIF20B) T1604 Uniprot
Q96Q89 (KIF20B) T1644 Uniprot
Q96R06 (SPAG5) S135 Uniprot
Q96R06 (SPAG5) S249 Uniprot
Q96RL1 (UIMC1) S677 Uniprot
Q96T23 (RSF1) S1375 Uniprot
Q96T88 (UHRF1) S639 Uniprot
Q99638 (RAD9A) S277 Uniprot
Q99638 (RAD9A) T292 Uniprot
Q99638 (RAD9A) S328 Uniprot
Q99638 (RAD9A) S336 Uniprot
Q99638 (RAD9A) T355 Uniprot
Q99661 (KIF2C) T537 Uniprot
Q99666 (RGPD6) S1275 Uniprot
Q99728 (BARD1) S148 Uniprot
Q99728 (BARD1) S251 Uniprot
Q99728 (BARD1) S288 Uniprot
Q99728 (BARD1) T299 Uniprot
Q9BPX3 (NCAPG) T308 Uniprot
Q9BPX3 (NCAPG) T332 Uniprot
Q9BPX3 (NCAPG) T931 Uniprot
Q9BXS6 (NUSAP1) T300 Uniprot
Q9BXS6 (NUSAP1) T338 Uniprot
Q9BYG3 (NIFK) T238 Uniprot
Q9GZM8 (NDEL1) T219 Uniprot
Q9GZM8 (NDEL1) S242 Uniprot
Q9GZM8 (NDEL1) T245 Uniprot
Q9GZV5 (WWTR1) S90 Uniprot
Q9GZV5 (WWTR1) S105 Uniprot
Q9GZV5 (WWTR1) T285 Uniprot
Q9GZV5 (WWTR1) T326 Uniprot
Q9GZV5 (WWTR1) T346 Uniprot
Q9H0H5 (RACGAP1) T588 Uniprot
Q9H1A4 (ANAPC1) T291 Uniprot
Q9H1A4 (ANAPC1) S355 Uniprot
Q9H1A4 (ANAPC1) S373 Uniprot
Q9H1A4 (ANAPC1) S377 Uniprot
Q9H1A4 (ANAPC1) T530 Uniprot
Q9H1A4 (ANAPC1) T537 Uniprot
Q9H1A4 (ANAPC1) S547 Uniprot
Q9H1A4 (ANAPC1) S555 Uniprot
Q9H1A4 (ANAPC1) S688 Uniprot
Q9H1A4 (ANAPC1) T701 Uniprot
Q9H1E3 (NUCKS1) S181 Uniprot
Q9H467 (CUEDC2) S110 Uniprot
Q9H4X1-1 (RGCC) T111 Uniprot
Q9H8V3 (ECT2) T373 Uniprot
Q9H8V3 (ECT2) T444 Uniprot
Q9H8V3-4 (ECT2) T815 Uniprot
Q9H8V3 (ECT2) T846 Uniprot
Q9H9S0 (NANOG) S71 Uniprot
Q9HCH0 (NCKAP5L) S436 Uniprot
Q9HCH0 (NCKAP5L) S447 Uniprot
Q9HCH0 (NCKAP5L) S466 Uniprot
Q9HCH0 (NCKAP5L) S473 Uniprot
Q9HCH0 (NCKAP5L) S567 Uniprot
Q9HCH0 (NCKAP5L) S573 Uniprot
Q9HCH0 (NCKAP5L) S763 Uniprot
Q9NQR1 (KMT5A) S100 Uniprot
Q9NQS7 (INCENP) T59 Uniprot
Q9NQS7 (INCENP) T412 Uniprot
Q9NQX3 (GPHN) S270 Uniprot
Q9NTG7 (SIRT3) T150 Uniprot
Q9NTG7 (SIRT3) S159 Uniprot
Q9NTI5 (PDS5B) T1370 Uniprot
Q9NXR1-2 (NDE1) T215 Uniprot
Q9NXR1-2 (NDE1) S239 Uniprot
Q9NXR1 (NDE1) T246 Uniprot
Q9NZH5 (PTTG2) S165 Uniprot
Q9NZJ0 (DTL) T464 Uniprot
Q9NZT2 (OGFR) S378 Uniprot
Q9UBF8 (PI4KB) S266 Uniprot
Q9UHD8-2 (SEPT9) T24 Uniprot
Q9UJ70 (NAGK) S76 Uniprot
Q9UJX2 (CDC23) T562 Uniprot
Q9UJX2 (CDC23) T565 Uniprot
Q9UJX5 (ANAPC4) S779 Uniprot
Q9UK53-2 (ING1) S126 Uniprot
Q9UKX7 (NUP50) S221 Uniprot
Q9ULC4 (MCTS1) S118 Uniprot
Q9ULW0 (TPX2) T72 Uniprot
Q9ULW0 (TPX2) S738 Uniprot
Q9UNZ2-1 (NSFL1C) S140 Uniprot
Q9UPP1 (PHF8) S69 Uniprot
Q9UPP1 (PHF8) S120 Uniprot
Q9UQ35 (SRRM2) T866 Uniprot
Q9UQ35 (SRRM2) T1413 Uniprot
Q9UQR0 (SCML2) T305 Uniprot
Q9UQR0 (SCML2) S511 Uniprot
Q9UQR0 (SCML2) S590 Uniprot
Q9Y2I6 (NINL) S185 Uniprot
Q9Y2I6 (NINL) S589 Uniprot
Q9Y2V2 (CARHSP1) T23 Uniprot
Q9Y3Z3 (SAMHD1) T592 Uniprot
Q9Y4H2 (IRS2) S391 Uniprot
Q9Y5N6 (ORC6) T195 Uniprot
Q9Y5T5 (USP16) S552 Uniprot
Q9Y6I3-3 (EPN1) S357 Uniprot
Q9Y6I3 (EPN1) S382 Uniprot
Q9Y6Q9 (NCOA3) S728 Uniprot
Q9Y6Q9 (NCOA3) S867 Uniprot
Substrate Site Source
A1KXE4 (FAM168B) T57 Uniprot
O00267 (SUPT5H) S666 Uniprot
O00267 (SUPT5H) T775 Uniprot
O00267 (SUPT5H) T791 Uniprot
O00311 (CDC7) T376 Uniprot
O00429 (DNM1L) S616 Uniprot
O00571 (DDX3X) T204 Uniprot
O14497 (ARID1A) S363 Uniprot
O14497 (ARID1A) S1320 Uniprot
O14497 (ARID1A) S1322 Uniprot
O14686 (KMT2D) T5374 Uniprot
O14757 (CHEK1) S286 Uniprot
O14757 (CHEK1) S301 Uniprot
O15014 (ZNF609) T722 Uniprot
O15350-1 (TP73) T86 Uniprot
O43395 (PRPF3) T167 Uniprot
O43463 (SUV39H1) S391 Uniprot
O43663-1 (PRC1) T470 Uniprot
O43663-1 (PRC1) T481 Uniprot
O60271 (SPAG9) T586 Uniprot
O60504 (SORBS3) T562 Uniprot
O60504 (SORBS3) S563 Uniprot
O60934 (NBN) S432 Uniprot
O75152 (ZC3H11A) T321 Uniprot
O75179 (ANKRD17) S2042 Uniprot
O75179 (ANKRD17) S2044 Uniprot
O75179 (ANKRD17) S2045 Uniprot
O75179 (ANKRD17) S2401 Uniprot
O75469 (NR1I2) S114 Uniprot
O75469 (NR1I2) T133 Uniprot
O75469 (NR1I2) T135 Uniprot
O75469 (NR1I2) S167 Uniprot
O75469 (NR1I2) S200 Uniprot
O75469 (NR1I2) S350 Uniprot
O75533 (SF3B1) T142 Uniprot
O75533 (SF3B1) T211 Uniprot
O75533 (SF3B1) T244 Uniprot
O75533 (SF3B1) T248 Uniprot
O75533 (SF3B1) S259 Uniprot
O75533 (SF3B1) T261 Uniprot
O75533 (SF3B1) T267 Uniprot
O75533 (SF3B1) T313 Uniprot
O75533 (SF3B1) T354 Uniprot
O75533 (SF3B1) T426 Uniprot
O75533 (SF3B1) T432 Uniprot
O75533 (SF3B1) T434 Uniprot
O75533 (SF3B1) T436 Uniprot
O75553 (DAB1) S524 Uniprot
O75616 (ERAL1) S173 Uniprot
O94776 (MTA2) S435 Uniprot
O94776 (MTA2) T438 Uniprot
O94776 (MTA2) T439 Uniprot
O94776 (MTA2) S440 Uniprot
O95251 (KAT7) T88 Uniprot
O95295 (SNAPIN) S133 Uniprot
P03372 (ESR1) S104 Uniprot
P03372 (ESR1) S106 Uniprot
P03372 (ESR1) S118 Uniprot
P03372 (ESR1) S294 Uniprot
P04183 (TK1) S13 Uniprot
P04637 (TP53) S313 Uniprot
P04637 (TP53) S314 Uniprot
P04637 (TP53) S315 Uniprot
P04637-1 (TP53) S392 Uniprot
P06400 (RB1) S230 Uniprot
P06400 (RB1) S249 Uniprot
P06400 (RB1) T252 Uniprot
P06400 (RB1) T356 Uniprot
P06400 (RB1) T373 Uniprot
P06400 (RB1) S567 Uniprot
P06400 (RB1) S608 Uniprot
P06400 (RB1) S612 Uniprot
P06400 (RB1) S807 Uniprot
P06400 (RB1) S811 Uniprot
P06400 (RB1) T821 Uniprot
P06400 (RB1) T826 Uniprot
P06401 (PGR) S20 Uniprot
P06401 (PGR) S25 Uniprot
P06401 (PGR) S162 Uniprot
P06401 (PGR) S190 Uniprot
P06401 (PGR) S213 Uniprot
P06401 (PGR) S294 Uniprot
P06401 (PGR) S400 Uniprot
P06401 (PGR) T430 Uniprot
P06401 (PGR) S554 Uniprot
P06401 (PGR) S676 Uniprot
P06748 (NPM1) S70 Uniprot
P06748 (NPM1) T199 Uniprot
P06748 (NPM1) T234 Uniprot
P08621 (SNRNP70) S226 Uniprot
P08670 (VIM) S55 Uniprot
P08670 (VIM) S56 Uniprot
P10244-1 (MYBL2) T266 Uniprot
P10244-1 (MYBL2) S393 Uniprot
P10244-1 (MYBL2) T405 Uniprot
P10244-1 (MYBL2) T440 Uniprot
P10244-1 (MYBL2) T444 Uniprot
P10244-1 (MYBL2) S452 Uniprot
P10244-1 (MYBL2) T487 Uniprot
P10244-1 (MYBL2) T494 Uniprot
P10244-1 (MYBL2) T515 Uniprot
P10244-1 (MYBL2) T518 Uniprot
P10244 (MYBL2) T520 Uniprot
P10244-1 (MYBL2) S577 Uniprot
P10412 (HIST1H1E) T18 Uniprot
P10644 (PRKAR1A) S83 Uniprot
P10644-1 (PRKAR1A) T209 Uniprot
P12268 (IMPDH2) S122 Uniprot
P12270 (TPR) S1676 Uniprot
P12270 (TPR) T1677 Uniprot
P12270 (TPR) S1679 Uniprot
P12272-1 (PTHLH) T108 Uniprot
P12272 (PTHLH) T121 Uniprot
P12956 (XRCC6) T455 Uniprot
P13051 (UNG) S12 Uniprot
P13639 (EEF2) T435 Uniprot
P13639 (EEF2) S595 Uniprot
P14859 (POU2F1) S269 Uniprot
P14859 (POU2F1) T270 Uniprot
P15172 (MYOD1) S200 Uniprot
P15336 (ATF2) T116 Uniprot
P15923 (TCF3) S139 Uniprot
P15923 (TCF3) S245 Uniprot
P15927 (RPA2) S23 Uniprot
P15927 (RPA2) S29 Uniprot
P15927 (RPA2) S33 Uniprot
P16949 (STMN1) S25 Uniprot
P16949 (STMN1) S38 Uniprot
P17096 (HMGA1) S36 Uniprot
P17480 (UBTF) S389 Uniprot
P17480 (UBTF) S484 Uniprot
P17676 (CEBPB) T235 Uniprot
P17706 (PTPN2) S304 Uniprot
P18858 (LIG1) S51 Uniprot
P18858 (LIG1) S76 Uniprot
P18858 (LIG1) S91 Uniprot
P18858 (LIG1) T195 Uniprot
P18858 (LIG1) T197 Uniprot
P18858 (LIG1) T233 Uniprot
P18887 (XRCC1) S226 Uniprot
P18887 (XRCC1) S229 Uniprot
P19338 (NCL) S67 Uniprot
P19338 (NCL) T76 Uniprot
P19338 (NCL) T121 Uniprot
P20248 (CCNA2) S154 Uniprot
P22234 (PAICS) S27 Uniprot
P22674 (CCNO) S81 Uniprot
P23258 (TUBG1) S80 Uniprot
P23396 (RPS3) T220 Uniprot
P23396 (RPS3) T221 Uniprot
P23511-2 (NFYA) S291 Uniprot
P23511-2 (NFYA) S297 Uniprot
P23511-1 (NFYA) S320 Uniprot
P23511-1 (NFYA) S326 Uniprot
P23771 (GATA3) T156 Uniprot
P24864 (CCNE1) S387 Uniprot
P24864 (CCNE1) T395 Uniprot
P24864 (CCNE1) S399 Uniprot
P24928 (POLR2A) S1882 Uniprot
P24928 (POLR2A) T1884 Uniprot
P24928 (POLR2A) T1885 Uniprot
P24928 (POLR2A) T1915 Uniprot
P24928 (POLR2A) S1917 Uniprot
P24941-1 (CDK2) Y19 Uniprot
P24941 (CDK2) T160 Uniprot
P25054 (APC) S1360 Uniprot
P25098 (GRK2) S670 Uniprot
P25205 (MCM3) T722 Uniprot
P26358 (DNMT1) S154 Uniprot
P28324 (ELK4) T194 Uniprot
P28324 (ELK4) S387 Uniprot
P28749 (RBL1) S640 Uniprot
P28749 (RBL1) S650 Uniprot
P29350 (PTPN6) S591 Uniprot
P29374 (ARID4A) S864 Uniprot
P29374 (ARID4A) S1007 Uniprot
P29374 (ARID4A) T1124 Uniprot
P29374 (ARID4A) S1140 Uniprot
P29374 (ARID4A) S1145 Uniprot
P29590 (PML) S518 Uniprot
P29966 (MARCKS) S26 Uniprot
P29966 (MARCKS) S27 Uniprot
P29966 (MARCKS) S118 Uniprot
P29966 (MARCKS) S131 Uniprot
P29966 (MARCKS) S132 Uniprot
P29966 (MARCKS) T133 Uniprot
P29966 (MARCKS) S134 Uniprot
P29966 (MARCKS) S135 Uniprot
P29966 (MARCKS) S147 Uniprot
P29966 (MARCKS) T150 Uniprot
P30050 (RPL12) S38 Uniprot
P30291 (WEE1) S123 Uniprot
P30304 (CDC25A) S263 Uniprot
P30307 (CDC25C) S214 Uniprot
P31350 (RRM2) S20 Uniprot
P31350 (RRM2) T33 Uniprot
P31943 (HNRNPH1) S104 Uniprot
P32322 (PYCR1) S301 Uniprot
P32322 (PYCR1) S303 Uniprot
P33981-1 (TTK) T468 Uniprot
P33991 (MCM4) S3 Uniprot
P33991 (MCM4) T7 Uniprot
P33991 (MCM4) T19 Uniprot
P33991 (MCM4) S32 Uniprot
P33991 (MCM4) T53 Uniprot
P33991 (MCM4) S54 Uniprot
P33991 (MCM4) T110 Uniprot
P33993 (MCM7) S121 Uniprot
P33993 (MCM7) S365 Uniprot
P35269 (GTF2F1) T445 Uniprot
P35269 (GTF2F1) T446 Uniprot
P35611 (ADD1) S613 Uniprot
P35611 (ADD1) T614 Uniprot
P38398-3 (BRCA1) S393 Uniprot
P38398-8 (BRCA1) S1450 Uniprot
P38398 (BRCA1) S1497 Uniprot
P38432 (COIL) S184 Uniprot
P38919 (EIF4A3) T163 Uniprot
P38936 (CDKN1A) S130 Uniprot
P42677 (RPS27) S27 Uniprot
P46013 (MKI67) S357 Uniprot
P46527 (CDKN1B) T187 Uniprot
P48200 (IREB2) S157 Uniprot
P48634-2 (PRRC2A) T1340 Uniprot
P49454 (CENPF) T154 Uniprot
P49454 (CENPF) S156 Uniprot
P49459-2 (UBE2A) S90 Uniprot
P49459 (UBE2A) S120 Uniprot
P49736 (MCM2) S13 Uniprot
P49736 (MCM2) S27 Uniprot
P49736 (MCM2) S40 Uniprot
P49736 (MCM2) S41 Uniprot
P49736 (MCM2) S53 Uniprot
P49736 (MCM2) S108 Uniprot
P49736 (MCM2) S139 Uniprot
P49790 (NUP153) S386 Uniprot
P49790 (NUP153) T388 Uniprot
P49915 (GMPS) S313 Uniprot
P49915 (GMPS) T318 Uniprot
P49916 (LIG3) T191 Uniprot
P49916 (LIG3) S210 Uniprot
P49916 (LIG3) S913 Uniprot
P50570 (DNM2) S764 Uniprot
P50613 (CDK7) S164 Uniprot
P50613 (CDK7) T170 Uniprot
P50750 (CDK9) S90 Uniprot
P51649 (ALDH5A1) T181 Uniprot
P52948 (NUP98) S612 Uniprot
P54132 (BLM) S175 Uniprot
P54198-1 (HIRA) T555 Uniprot
P54198 (HIRA) T583 Uniprot
P54198 (HIRA) S584 Uniprot
P54198 (HIRA) T586 Uniprot
P54198 (HIRA) T588 Uniprot
P54198-1 (HIRA) S687 Uniprot
P55196 (AFDN) T1352 Uniprot
P55273 (CDKN2D) S76 Uniprot
P55273 (CDKN2D) T141 Uniprot
P55795 (HNRNPH2) S104 Uniprot
P57740 (NUP107) S10 Uniprot
P57740 (NUP107) S11 Uniprot
P61978 (HNRNPK) S216 Uniprot
P62136 (PPP1CA) T320 Uniprot
P62324 (BTG1) S159 Uniprot
P62913 (RPL11) T47 Uniprot
P78317 (RNF4) T26 Uniprot
P78317 (RNF4) T112 Uniprot
P78347 (GTF2I) T556 Uniprot
P78347 (GTF2I) T558 Uniprot
P82970 (HMGN5) T31 Uniprot
P84022-1 (SMAD3) T8 Uniprot
P84022-1 (SMAD3) T179 Uniprot
P84022-1 (SMAD3) S204 Uniprot
P84022-1 (SMAD3) S208 Uniprot
P84022-1 (SMAD3) S213 Uniprot
Q00059 (TFAM) T122 Uniprot
Q00059 (TFAM) S124 Uniprot
Q00536 (CDK16) S138 Uniprot
Q01167 (FOXK2) S373 Uniprot
Q01167 (FOXK2) S428 Uniprot
Q01196 (RUNX1) S249 Uniprot
Q01196 (RUNX1) S266 Uniprot
Q01196 (RUNX1) T273 Uniprot
Q01196-8 (RUNX1) S276 Uniprot
Q01196-8 (RUNX1) S293 Uniprot
Q01196-8 (RUNX1) T300 Uniprot
Q01196-8 (RUNX1) S303 Uniprot
Q02363 (ID2) S5 Uniprot
Q02535 (ID3) S5 Uniprot
Q02539 (HIST1H1A) T152 Uniprot
Q03252 (LMNB2) S37 Uniprot
Q03252 (LMNB2) T39 Uniprot
Q04323 (UBXN1) S199 Uniprot
Q04323 (UBXN1) S200 Uniprot
Q04323 (UBXN1) S202 Uniprot
Q04637 (EIF4G1) T647 Uniprot
Q05516 (ZBTB16) S197 Uniprot
Q05516 (ZBTB16) T282 Uniprot
Q05519 (SRSF11) S207 Uniprot
Q05519 (SRSF11) S209 Uniprot
Q05682 (CALD1) T730 Uniprot
Q06830 (PRDX1) T90 Uniprot
Q07820 (MCL1) S64 Uniprot
Q07820 (MCL1) T92 Uniprot
Q07820 (MCL1) S121 Uniprot
Q07820 (MCL1) T163 Uniprot
Q08050-2 (FOXM1) T596 Uniprot
Q08050-1 (FOXM1) T611 Uniprot
Q08050-3 (FOXM1) T649 Uniprot
Q08999 (RBL2) S413 Uniprot
Q08999 (RBL2) T417 Uniprot
Q08999 (RBL2) S639 Uniprot
Q08999 (RBL2) T642 Uniprot
Q08999 (RBL2) S662 Uniprot
Q08999 (RBL2) S688 Uniprot
Q08999 (RBL2) T694 Uniprot
Q08999 (RBL2) S952 Uniprot
Q08999 (RBL2) S1044 Uniprot
Q08999 (RBL2) S1068 Uniprot
Q08999 (RBL2) S1080 Uniprot
Q08999 (RBL2) T1097 Uniprot
Q08999 (RBL2) S1112 Uniprot
Q0VD86 (INCA1) S23 Uniprot
Q0VD86 (INCA1) T182 Uniprot
Q0VD86 (INCA1) S191 Uniprot
Q12778 (FOXO1) S249 Uniprot
Q12800 (TFCP2) T258 Uniprot
Q12800 (TFCP2) S289 Uniprot
Q12800 (TFCP2) S291 Uniprot
Q12800 (TFCP2) S309 Uniprot
Q12888 (TP53BP1) T1609 Uniprot
Q12888 (TP53BP1) T1612 Uniprot
Q12906 (ILF3) T363 Uniprot
Q12906 (ILF3) T364 Uniprot
Q12906 (ILF3) T368 Uniprot
Q12906 (ILF3) S382 Uniprot
Q12906 (ILF3) S384 Uniprot
Q12948 (FOXC1) T68 Uniprot
Q12959 (DLG1) S158 Uniprot
Q12959 (DLG1) S443 Uniprot
Q13118 (KLF10) S206 Uniprot
Q13242 (SRSF9) S216 Uniprot
Q13309 (SKP2) S64 Uniprot
Q13330 (MTA1) S522 Uniprot
Q13352 (ITGB3BP) S33 Uniprot
Q13352 (ITGB3BP) S46 Uniprot
Q13415 (ORC1) S258 Uniprot
Q13415 (ORC1) S273 Uniprot
Q13415 (ORC1) T375 Uniprot
Q13416 (ORC2) T116 Uniprot
Q13416 (ORC2) T226 Uniprot
Q13428 (TCOF1) S582 Uniprot
Q13428 (TCOF1) S583 Uniprot
Q13428 (TCOF1) S982 Uniprot
Q13428 (TCOF1) T983 Uniprot
Q13573 (SNW1) S224 Uniprot
Q13573 (SNW1) S232 Uniprot
Q13573 (SNW1) S234 Uniprot
Q14103 (HNRNPD) T193 Uniprot
Q14194 (CRMP1) T509 Uniprot
Q14195 (DPYSL3) T507 Uniprot
Q14195 (DPYSL3) T508 Uniprot
Q14195 (DPYSL3) T509 Uniprot
Q14202 (ZMYM3) T826 Uniprot
Q14207 (NPAT) S775 Uniprot
Q14207 (NPAT) S779 Uniprot
Q14207 (NPAT) S1100 Uniprot
Q14207 (NPAT) T1270 Uniprot
Q14207 (NPAT) T1350 Uniprot
Q14247 (CTTN) S405 Uniprot
Q14676 (MDC1) T341 Uniprot
Q14676 (MDC1) S1068 Uniprot
Q14676 (MDC1) T1630 Uniprot
Q14807 (KIF22) S462 Uniprot
Q14807 (KIF22) T463 Uniprot
Q14839 (CHD4) T529 Uniprot
Q14839 (CHD4) S531 Uniprot
Q14980 (NUMA1) T1776 Uniprot
Q14CB8 (ARHGAP19) T476 Uniprot
Q14CB8 (ARHGAP19) T478 Uniprot
Q15003 (NCAPH) T49 Uniprot
Q15047 (SETDB1) S1066 Uniprot
Q15398 (DLGAP5) T342 Uniprot
Q15398 (DLGAP5) T344 Uniprot
Q15554 (TERF2) S365 Uniprot
Q15648 (MED1) S1207 Uniprot
Q15648 (MED1) T1215 Uniprot
Q15717 (ELAVL1) S202 Uniprot
Q15796 (SMAD2) T8 Uniprot
Q15813 (TBCE) S495 Uniprot
Q15910 (EZH2) T345 Uniprot
Q15910 (EZH2) T416 Uniprot
Q15942 (ZYX) S303 Uniprot
Q15942 (ZYX) T306 Uniprot
Q15942 (ZYX) S308 Uniprot
Q16204 (CCDC6) S244 Uniprot
Q2NL82 (TSR1) T490 Uniprot
Q32P44 (EML3) T881 Uniprot
Q32P44 (EML3) S883 Uniprot
Q4VCS5 (AMOT) S847 Uniprot
Q4VCS5 (AMOT) T848 Uniprot
Q4VCS5 (AMOT) S852 Uniprot
Q4VCS5 (AMOT) S855 Uniprot
Q53ET0 (CRTC2) S433 Uniprot
Q53GL7 (PARP10) T101 Uniprot
Q53H80 (AKIRIN2) S18 Uniprot
Q53H80 (AKIRIN2) S21 Uniprot
Q5JTV8 (TOR1AIP1) S143 Uniprot
Q5JTZ9 (AARS2) T673 Uniprot
Q5T5Y3 (CAMSAP1) S1080 Uniprot
Q5TZA2-2 (CROCC) S763 Uniprot
Q5TZA2 (CROCC) S1460 Uniprot
Q6P1N0 (CC2D1A) S208 Uniprot
Q6PKG0 (LARP1) T303 Uniprot
Q6PKG0 (LARP1) T649 Uniprot
Q6PKG0 (LARP1) S774 Uniprot
Q6UB35 (MTHFD1L) S357 Uniprot
Q6Y7W6-3 (GIGYF2) S30 Uniprot
Q6Y7W6-3 (GIGYF2) S614 Uniprot
Q71UM5 (RPS27L) S27 Uniprot
Q7KZ85 (SUPT6H) T1523 Uniprot
Q7Z2Z1 (TICRR) T969 Uniprot
Q7Z2Z1 (TICRR) S1001 Uniprot
Q7Z417 (NUFIP2) T219 Uniprot
Q7Z417 (NUFIP2) T220 Uniprot
Q86T82 (USP37) S628 Uniprot
Q86U86 (PBRM1) S25 Uniprot
Q86U86 (PBRM1) S27 Uniprot
Q86U86 (PBRM1) T28 Uniprot
Q86U86 (PBRM1) S32 Uniprot
Q86VM9 (ZC3H18) S613 Uniprot
Q86VM9 (ZC3H18) S618 Uniprot
Q86VM9 (ZC3H18) S795 Uniprot
Q86VM9 (ZC3H18) T796 Uniprot
Q86WB0 (ZC3HC1) S394 Uniprot
Q86WB0 (ZC3HC1) S395 Uniprot
Q86XL3 (ANKLE2) S528 Uniprot
Q86YP4 (GATAD2A) S598 Uniprot
Q86YP4 (GATAD2A) S600 Uniprot
Q8IWS0 (PHF6) S154 Uniprot
Q8IWS0 (PHF6) S155 Uniprot
Q8IXJ6-2 (SIRT2) S331 Uniprot
Q8IXJ6 (SIRT2) S368 Uniprot
Q8IXM2 (BAP18) S96 Uniprot
Q8IXM2 (BAP18) S106 Uniprot
Q8IXM2 (BAP18) S110 Uniprot
Q8IXQ3 (C9orf40) S67 Uniprot
Q8IXQ3 (C9orf40) S69 Uniprot
Q8IXQ3 (C9orf40) S71 Uniprot
Q8IYB3 (SRRM1) S769 Uniprot
Q8IYB3 (SRRM1) S773 Uniprot
Q8IZL8 (PELP1) S477 Uniprot
Q8IZL8 (PELP1) S991 Uniprot
Q8N3F8 (MICALL1) T469 Uniprot
Q8N3F8 (MICALL1) S470 Uniprot
Q8N3F8 (MICALL1) S471 Uniprot
Q8N3F8 (MICALL1) S619 Uniprot
Q8N3F8 (MICALL1) S620 Uniprot
Q8N3F8 (MICALL1) S621 Uniprot
Q8NBZ0 (INO80E) S154 Uniprot
Q8NFC6 (BOD1L1) S2905 Uniprot
Q8NFC6 (BOD1L1) S2907 Uniprot
Q8NFC6 (BOD1L1) S2908 Uniprot
Q8NFC6 (BOD1L1) S2909 Uniprot
Q8TAP6 (CEP76) S83 Uniprot
Q8TAP8 (PPP1R35) S45 Uniprot
Q8TAP8 (PPP1R35) S47 Uniprot
Q8TAP8 (PPP1R35) S52 Uniprot
Q8WUM0 (NUP133) S27 Uniprot
Q8WUM0 (NUP133) T28 Uniprot
Q8WUM0 (NUP133) S50 Uniprot
Q8WWY3 (PRPF31) S445 Uniprot
Q8WWY3 (PRPF31) S446 Uniprot
Q8WWY3 (PRPF31) S450 Uniprot
Q8WWY3 (PRPF31) S451 Uniprot
Q8WWY3 (PRPF31) T455 Uniprot
Q8WXE1 (ATRIP) S224 Uniprot
Q8WXE1 (ATRIP) S239 Uniprot
Q8WYH8 (ING5) T152 Uniprot
Q92547 (TOPBP1) T848 Uniprot
Q92620 (DHX38) S232 Uniprot
Q92620 (DHX38) S234 Uniprot
Q92785 (DPF2) T176 Uniprot
Q92785 (DPF2) T248 Uniprot
Q92785 (DPF2) S251 Uniprot
Q969V6 (MRTFA) T573 Uniprot
Q96C19 (EFHD2) S74 Uniprot
Q96C19 (EFHD2) S76 Uniprot
Q96C36 (PYCR2) T301 Uniprot
Q96C36 (PYCR2) S303 Uniprot
Q96C36 (PYCR2) S304 Uniprot
Q96E09 (FAM122A) S143 Uniprot
Q96EA4 (SPDL1) S513 Uniprot
Q96EA4 (SPDL1) S515 Uniprot
Q96II8 (LRCH3) S419 Uniprot
Q96KS0 (EGLN2) S130 Uniprot
Q96RK0 (CIC) T583 Uniprot
Q96RK0 (CIC) S585 Uniprot
Q96T88 (UHRF1) S661 Uniprot
Q96T88-2 (UHRF1) S674 Uniprot
Q99459 (CDC5L) S410 Uniprot
Q99459 (CDC5L) T411 Uniprot
Q99504 (EYA3) S266 Uniprot
Q99504 (EYA3) T268 Uniprot
Q99504 (EYA3) T269 Uniprot
Q99504 (EYA3) S271 Uniprot
Q99607 (ELF4) T643 Uniprot
Q99607 (ELF4) S648 Uniprot
Q99626 (CDX2) S283 Uniprot
Q99626 (CDX2) S287 Uniprot
Q99626 (CDX2) S291 Uniprot
Q99638 (RAD9A) S328 Uniprot
Q99708 (RBBP8) T315 Uniprot
Q99708 (RBBP8) T847 Uniprot
Q99728 (BARD1) S148 Uniprot
Q99728 (BARD1) S251 Uniprot
Q99728 (BARD1) S288 Uniprot
Q99728 (BARD1) T299 Uniprot
Q99741 (CDC6) S54 Uniprot
Q99741 (CDC6) S74 Uniprot
Q99741 (CDC6) S106 Uniprot
Q9BUJ2 (HNRNPUL1) S716 Uniprot
Q9BUJ2 (HNRNPUL1) S718 Uniprot
Q9BVC5 (C2orf49) S193 Uniprot
Q9BVC5 (C2orf49) T197 Uniprot
Q9BVC5 (C2orf49) T198 Uniprot
Q9BXR0 (QTRT1) S139 Uniprot
Q9BY44 (EIF2A) S517 Uniprot
Q9BY44 (EIF2A) T518 Uniprot
Q9BZL4 (PPP1R12C) S509 Uniprot
Q9C0C2 (TNKS1BP1) S435 Uniprot
Q9C0C2 (TNKS1BP1) S437 Uniprot
Q9C0C2 (TNKS1BP1) S691 Uniprot
Q9C0C9 (UBE2O) S836 Uniprot
Q9C0D5 (TANC1) S1564 Uniprot
Q9H1E3 (NUCKS1) S181 Uniprot
Q9H211 (CDT1) T29 Uniprot
Q9H211 (CDT1) S31 Uniprot
Q9H2G4 (TSPYL2) S20 Uniprot
Q9H2G4 (TSPYL2) T340 Uniprot
Q9H3D4 (TP63) S477 Uniprot
Q9H3D4 (TP63) T491 Uniprot
Q9H3D4 (TP63) S560 Uniprot
Q9H3P2 (NELFA) T157 Uniprot
Q9H425 (C1orf198) S37 Uniprot
Q9H6K1 (ILRUN) S215 Uniprot
Q9H7L9 (SUDS3) S234 Uniprot
Q9H7L9 (SUDS3) S236 Uniprot
Q9H7L9 (SUDS3) S237 Uniprot
Q9H7L9 (SUDS3) T244 Uniprot
Q9H8Y8 (GORASP2) T225 Uniprot
Q9H910 (JPT2) S97 Uniprot
Q9H9C1 (VIPAS39) S130 Uniprot
Q9H9C1 (VIPAS39) T132 Uniprot
Q9NP87 (POLM) S372 Uniprot
Q9NPF5 (DMAP1) T445 Uniprot
Q9NPF5 (DMAP1) S448 Uniprot
Q9NS91 (RAD18) S99 Uniprot
Q9NSV4 (DIAPH3) T19 Uniprot
Q9NTI5 (PDS5B) S1369 Uniprot
Q9NTI5 (PDS5B) T1370 Uniprot
Q9NXR1 (NDE1) T228 Uniprot
Q9NYB0 (TERF2IP) S203 Uniprot
Q9NYV6 (RRN3) S44 Uniprot
Q9NZJ0 (DTL) S428 Uniprot
Q9NZJ0 (DTL) T429 Uniprot
Q9P270 (SLAIN2) S48 Uniprot
Q9P2B4 (CTTNBP2NL) S563 Uniprot
Q9P2D1 (CHD7) S2471 Uniprot
Q9P2D1 (CHD7) T2472 Uniprot
Q9P2N5 (RBM27) T796 Uniprot
Q9P2N5 (RBM27) S798 Uniprot
Q9UEY8 (ADD3) S423 Uniprot
Q9UFC0 (LRWD1) S243 Uniprot
Q9UFC0 (LRWD1) S245 Uniprot
Q9UGP5 (POLL) S167 Uniprot
Q9UGP5 (POLL) S177 Uniprot
Q9UGP5 (POLL) S230 Uniprot
Q9UGP5 (POLL) T553 Uniprot
Q9UIF9 (BAZ2A) S509 Uniprot
Q9UIF9 (BAZ2A) S1183 Uniprot
Q9UIF9 (BAZ2A) S1184 Uniprot
Q9UJU6 (DBNL) S274 Uniprot
Q9UJU6 (DBNL) S275 Uniprot
Q9UKM9 (RALY) S135 Uniprot
Q9UKY1 (ZHX1) T568 Uniprot
Q9ULW0 (TPX2) T72 Uniprot
Q9ULX3 (NOB1) T121 Uniprot
Q9UM11 (FZR1) S40 Uniprot
Q9UM11 (FZR1) T121 Uniprot
Q9UM11 (FZR1) S151 Uniprot
Q9UM11 (FZR1) S163 Uniprot
Q9UPN3 (MACF1) T7213 Uniprot
Q9UQR0 (SCML2) T305 Uniprot
Q9UQR0 (SCML2) S511 Uniprot
Q9UQR0 (SCML2) S590 Uniprot
Q9Y266 (NUDC) S136 Uniprot
Q9Y266 (NUDC) S139 Uniprot
Q9Y2J2 (EPB41L3) T466 Uniprot
Q9Y2J2 (EPB41L3) T467 Uniprot
Q9Y2J2 (EPB41L3) S960 Uniprot
Q9Y2J2 (EPB41L3) S962 Uniprot
Q9Y2K7 (KDM2A) T550 Uniprot
Q9Y2W1 (THRAP3) T874 Uniprot
Q9Y314 (NOSIP) S166 Uniprot
Q9Y314 (NOSIP) T168 Uniprot
Q9Y3Z3 (SAMHD1) T592 Uniprot
Q9Y676 (MRPS18B) S49 Uniprot

Research Backgrounds

Function:

Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CENPA, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, TPPP, UL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1, SAMHD1, SIRT2 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair. Reactivated after successful DNA repair through WIP1-dependent signaling leading to CDC25A/B/C-mediated dephosphorylation and restoring cell cycle progression. In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons. The phosphorylation of beta-tubulins regulates microtubule dynamics during mitosis. NEDD1 phosphorylation promotes PLK1-mediated NEDD1 phosphorylation and subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. In addition, CC2D1A phosphorylation regulates CC2D1A spindle pole localization and association with SCC1/RAD21 and centriole cohesion during mitosis. The phosphorylation of Bcl-xL/BCL2L1 after prolongated G2 arrest upon DNA damage triggers apoptosis. In contrast, CASP8 phosphorylation during mitosis prevents its activation by proteolysis and subsequent apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. CALD1 phosphorylation promotes Schwann cell migration during peripheral nerve regeneration. CDK1-cyclin-B complex phosphorylates NCKAP5L and mediates its dissociation from centrosomes during mitosis. Regulates the amplitude of the cyclic expression of the core clock gene ARNTL/BMAL1 by phosphorylating its transcriptional repressor NR1D1, and this phosphorylation is necessary for SCF(FBXW7)-mediated ubiquitination and proteasomal degradation of NR1D1.

(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry.

PTMs:

Phosphorylation at Thr-161 by CAK/CDK7 activates kinase activity. Phosphorylation at Thr-14 and Tyr-15 by PKMYT1 prevents nuclear translocation. Phosphorylation at Tyr-15 by WEE1 and WEE2 inhibits the protein kinase activity and acts as a negative regulator of entry into mitosis (G2 to M transition). Phosphorylation by PKMYT1 and WEE1 takes place during mitosis to keep CDK1-cyclin-B complexes inactive until the end of G2. By the end of G2, PKMYT1 and WEE1 are inactivated, but CDC25A and CDC25B are activated. Dephosphorylation by active CDC25A and CDC25B at Thr-14 and Tyr-15, leads to CDK1 activation at the G2-M transition. Phosphorylation at Tyr-15 by WEE2 during oogenesis is required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, leading to prevent meiotic reentry. Phosphorylation by WEE2 is also required for metaphase II exit during egg activation to ensure exit from meiosis in oocytes and promote pronuclear formation. Phosphorylated at Tyr-4 by PKR/EIF2AK2 upon genotoxic stress. This phosphorylation triggers CDK1 polyubiquitination and subsequent proteolysis, thus leading to G2 arrest. In response to UV irradiation, phosphorylation at Tyr-15 by PRKCD activates the G2/M DNA damage checkpoint.

Polyubiquitinated upon genotoxic stress.

Subcellular Location:

Nucleus. Cytoplasm. Mitochondrion. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle.
Note: Cytoplasmic during the interphase. Colocalizes with SIRT2 on centrosome during prophase and on splindle fibers during metaphase of the mitotic cell cycle. Reversibly translocated from cytoplasm to nucleus when phosphorylated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 2 is found in breast cancer tissues.

Subunit Structure:

Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with cyclins-B (CCNB1, CCNB2 and CCNB3) to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Can also form CDK1-cylin-D and CDK1-cyclin-E complexes that phosphorylate RB1 in vitro. Binds to RB1 and other transcription factors such as FOXO1 and RUNX2. Promotes G2-M transition when in complex with a cyclin-B. Interacts with DLGAP5. Binds to the CDK inhibitors CDKN1A/p21 and CDKN1B/p27. Isoform 2 is unable to complex with cyclin-B1 and also fails to bind to CDKN1A/p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase. Associates with cyclins-A and B1 during S-phase in regenerating hepatocytes. Interacts with FANCC. Interacts with CEP63; this interaction recruits CDK1 to centrosomes. Interacts with CENPA. Interacts with NR1D1. Interacts with proteasome subunit PSMA8; to participate in meiosis progression during spermatogenesis (By similarity).

Family&Domains:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Function:

Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). Phosphorylates CDK2AP2. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks.

PTMs:

Phosphorylated at Thr-160 by CDK7 in a CAK complex. Phosphorylation at Thr-160 promotes kinase activity, whereas phosphorylation at Tyr-15 by WEE1 reduces slightly kinase activity. Phosphorylated on Thr-14 and Tyr-15 during S and G2 phases before being dephosphorylated by CDC25A.

Nitrosylated after treatment with nitric oxide (DETA-NO).

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Nucleus>Cajal body. Cytoplasm. Endosome.
Note: Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1 (By similarity). Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Interaction with SPDYA promotes kinase activation via a conformation change that alleviates obstruction of the substrate-binding cleft by the T-loop. Found in a complex with both SPDYA and CDKN1B/KIP1. Binds to RB1 and CDK7. Binding to CDKN1A (p21) leads to CDK2/cyclin E inactivation at the G1-S phase DNA damage checkpoint, thereby arresting cells at the G1-S transition during DNA repair. Associated with PTPN6 and beta-catenin/CTNNB1. Interacts with CACUL1. May interact with CEP63. Interacts with ANKRD17. Interacts with CEBPA (when phosphorylated). Forms a ternary complex with CCNA2 and CDKN1B; CDKN1B inhibits the kinase activity of CDK2 through conformational rearrangements. Interacts with cyclins A, B1, B3, D, or E. Interacts with CDK2AP2.

Family&Domains:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

References

1). Qu X et al. Sinomenine Inhibits the Growth of Ovarian Cancer Cells Through the Suppression of Mitosis by Down-Regulating the Expression and the Activity of CDK1. Onco Targets Ther 2021 Feb 5;14:823-834. (PubMed: 33574676) [IF=4.345]

Application: WB    Species: Human    Sample: HeyA8 cells

Figure 5 Sinomenine suppressed G2/M transition in HeyA8 cells by inhibiting the expression and the activity of CDK1. (A and B) The cell cycle transition of HeyA8 cells was examined by flow cytometry assay (A). The results showed that the percentage of cells in the G2/M phase was decreased significantly after treatment with sinomenine for 48 hours (B). Each value represents the mean ± SD for triplicate samples. ***P<0.001, Student’s t-test. (C and D) The percentage of M phase cells were analyzed by flow cytometry assay staining with P-Histone H3 (Ser10) and PI (C). The results showed that the percentage of M phase in HeyA8 cells treated with sinomenine for 48 hours were obviously decreased (D). Each value represents the mean ± SD for triplicate samples. ***P<0.001, Student’s t-test. (E) The expression of specific proteins in different cell cycle phase were determined by Western blot. (F) The expression level of P-Histone H3 (ser10), which only expressed in the M phase, were clearly decreased in sinomenine treated HeyA8 cells. Each value represents the mean ± SD for triplicate samples. ***P<0.001, one-way ANOVA analysis. (G) The expression of CDK1 and the phosphorylation of CDK1 were detected by Western blot. (H) The expression level CDK1 and P-CDK1 (Thr161) were clearly decreased in sinomenine treated HeyA8 cells. Each value represents the mean ± SD for triplicate samples. **P<0.01, ***P<0.001, one-way ANOVA analysis.

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