COX11 Antibody | Affinity Biosciences

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Western blot analysis of COX11 expression in Rat muscle lysate.

Product:	COX11 Antibody
Catalog:	AF0601
Description:	Rabbit polyclonal antibody to COX11
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Horse, Rabbit, Dog
Mol.Wt.:	31kDa; 31kD(Calculated).
Uniprot:	Q9Y6N1
RRID:	AB_2834373

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Horse(80%), Rabbit(80%), Dog(80%)

Clonality:

Polyclonal

Specificity:

COX11 Antibody detects endogenous levels of total COX11.

RRID:

AB_2834373
Cite Format: Affinity Biosciences Cat# AF0601, RRID:AB_2834373.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

COX11; COX11 homolog; COX11 homolog, cytochrome c oxidase assembly protein (yeast); COX11_HUMAN; COX11P; Cytochrome c oxidase assembly protein COX11; Cytochrome c oxidase assembly protein COX11, mitochondrial; Cytochrome c oxidase subunit 11; mitochondrial;

Immunogens

Immunogen:

Uniprot:

Q9Y6N1(COX11_HUMAN) >>Visit HPA database.

Gene(ID):

COX11(1353)

Expression:

Q9Y6N1 COX11_HUMAN:

Ubiquitous.

Description:

Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be a heme A biosynthetic enzyme involved in COX formation, according to the yeast mutant studies. However, the studies in Rhodobacter sphaeroides suggest that this gene is not required for heme A biosynthesis, but required for stable formation of the Cu(B) and magnesium centers of COX. This human protein is predicted to contain a transmembrane domain localized in the mitochondrial inner membrane. A pseudogene COX11P has been found on chromosome 6.

Sequence:

Q9Y6N1 COX11_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGGLWRPGWRCVPFCGWRWIHPGSPTRAAERVEPFLRPEWSGTGGAERGLRWLGTWKRCSLRARHPALQPPRRPKSSNPFTRAQEEERRRQNKTTLTYVAAVAVGMLGASYAAVPLYRLYCQTTGLGGSAVAGHASDKIENMVPVKDRIIKISFNADVHASLQWNFRPQQTEIYVVPGETALAFYRAKNPTDKPVIGISTYNIVPFEAGQYFNKIQCFCFEEQRLNPQEEVDMPVFFYIDPEFAEDPRMIKVDLITLSYTFFEAKEGHKLPVPGYN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Dog

Rabbit

Pig

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6N1 As Substrate

Q9Y6N1

Site	PTM Type	Source
S24	Phosphorylation	Uniprot
K57	Ubiquitination	Uniprot
Y117	Phosphorylation	Uniprot
K138	Ubiquitination	Uniprot
K146	Ubiquitination	Uniprot
K188	Ubiquitination	Uniprot
K193	Ubiquitination	Uniprot
K265	Ubiquitination	Uniprot
K269	Ubiquitination	Uniprot

Research Backgrounds

Q9Y6N1_COX11_HUMAN

Function:

Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I.

Subcellular Location:

Mitochondrion inner membrane>Single-pass membrane protein>Intermembrane side.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with CNNM4/ACDP4.

Family&Domains:

Belongs to the COX11/CtaG family.

Research Fields

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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COX11 Antibody - #AF0601