MAP3K4 Antibody - #AF0818
Product: | MAP3K4 Antibody |
Catalog: | AF0818 |
Description: | Rabbit polyclonal antibody to MAP3K4 |
Application: | WB IHC |
Reactivity: | Human, Mouse |
Prediction: | Pig, Bovine, Horse, Rabbit, Dog, Chicken |
Mol.Wt.: | 181kDa; 182kD(Calculated). |
Uniprot: | Q9Y6R4 |
RRID: | AB_2834365 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0818, RRID:AB_2834365.
Fold/Unfold
dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4); FLJ42439; KIAA0213; M3K4_HUMAN; MAP three kinase 1; MAP/ERK kinase kinase 4; MAP3K4; MAPK/ERK kinase kinase 4; MAPKKK4; MEK kinase 4; MEKK 4; MEKK4; Mitogen-activated protein kinase kinase kinase 4; MTK1; predicted protein of HQ0412; PRO0412; RP3-473J16.4; SSK2/SSK22 MAP kinase kinase kinase, yeast, homolog of;
Immunogens
Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues.
- Q9Y6R4 M3K4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MREAAAALVPPPAFAVTPAAAMEEPPPPPPPPPPPPEPETESEPECCLAARQEGTLGDSACKSPESDLEDFSDETNTENLYGTSPPSTPRQMKRMSTKHQRNNVGRPASRSNLKEKMNAPNQPPHKDTGKTVENVEEYSYKQEKKIRAALRTTERDRKKNVQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSVPMPIARPARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSGFWLNRSNELIWLELQAWHAGRTINDQDFFLYTARQAIPDIINEILTFKVDYGSFAFVRDRAGFNGTSVEGQCKATPGTKIVGYSTHHEHLQRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEYEGDDTEGELKELESSTDESEEEQISDPRVPEIRQPIDNSFDIQSRDCISKKLERLESEDDSLGWGAPDWSTEAGFSRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGSLQRARIALVKNDRPVEFSEFPDPMWGSDYVQLSRTPPSSEEKCSAVSWEELKAMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLQEVLEDLEKPDCNIDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLEFGLQESCAEFWTSADDSSASDEIRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFRLSAPVRDLLDVLKSKQYVKVQIPGLENLQMFVPDTLAEEKSIILQLLNAAAGKDCSKDSDDVLIDAYLLLTKHGDRARDSEDSWGTWEAQPVKVVPQVETVDTLRSMQVDNLLLVVMQSAHLTIQRKAFQQSIEGLMTLCQEQTSSQPVIAKALQQLKNDALELCNRISNAIDRVDHMFTSEFDAEVDESESVTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPMKVPRCHSDPPNPHLIIPTPEGFSTRSMPSDARSHGSPAAAAAAAAAAVAASRPSPSGGDSVLPKSISSAHDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPRGDSSGSTRRSWELRTLISQSKDTASKLGPIEAIQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKDFLSHCLESDPKMRWTASQLLDHSFVKVCTDEE
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y6R4 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S63 | Phosphorylation | Uniprot | |
S66 | Phosphorylation | Uniprot | |
S72 | Phosphorylation | Uniprot | |
Y81 | Phosphorylation | Uniprot | |
S84 | Phosphorylation | Uniprot | |
S87 | Phosphorylation | Uniprot | |
K98 | Acetylation | Uniprot | |
Y138 | Phosphorylation | Uniprot | |
Y140 | Phosphorylation | Uniprot | |
S173 | Phosphorylation | Uniprot | |
S253 | Phosphorylation | Uniprot | |
S431 | Phosphorylation | Uniprot | |
S435 | Phosphorylation | Uniprot | |
Y442 | Phosphorylation | Uniprot | |
T447 | Phosphorylation | Uniprot | |
S456 | Phosphorylation | Uniprot | |
S457 | Phosphorylation | Uniprot | |
T458 | Phosphorylation | Uniprot | |
S461 | Phosphorylation | Uniprot | |
S481 | Phosphorylation | Uniprot | |
S499 | Phosphorylation | Uniprot | |
Y582 | Phosphorylation | Uniprot | |
S798 | Phosphorylation | Uniprot | |
S835 | Phosphorylation | Uniprot | |
K890 | Acetylation | Uniprot | |
S979 | Phosphorylation | Uniprot | |
S1068 | Phosphorylation | Uniprot | |
Y1076 | Phosphorylation | Uniprot | |
S1135 | Phosphorylation | Uniprot | |
S1148 | Phosphorylation | Uniprot | |
S1159 | Phosphorylation | Uniprot | |
S1178 | Phosphorylation | Uniprot | |
S1252 | Phosphorylation | Uniprot | |
S1268 | Phosphorylation | Uniprot | |
S1270 | Phosphorylation | Uniprot | |
T1271 | Phosphorylation | Uniprot | |
S1274 | Phosphorylation | Uniprot | |
S1282 | Phosphorylation | Uniprot | |
Y1405 | Phosphorylation | Uniprot | |
T1494 | Phosphorylation | Q9Y6R4 (MAP3K4) | Uniprot |
T1505 | Phosphorylation | Uniprot | |
Y1548 | Phosphorylation | Uniprot | |
Y1556 | Phosphorylation | Uniprot | |
K1575 | Ubiquitination | Uniprot | |
S1579 | Phosphorylation | Uniprot | |
S1584 | Phosphorylation | Uniprot |
Research Backgrounds
Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.
Cytoplasm>Perinuclear region.
Note: Localized in perinuclear vesicular-like structures, probably Golgi-associated vesicles.
Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues.
Monomer and homodimer. Homodimerization enhances kinase activity. Interacts with TRAF4; this promotes homodimerization. Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts with AXIN1 and DIXDC1; interaction with DIXDC1 prevents interaction with AXIN1. Interacts with GADD45 and MAP2K6. Interacts with ZFP36; this interaction enhances the association with SH3KBP1/CIN85. Interacts with SH3KBP1; this interaction enhances the association with ZFP36. Interacts with CDC42 (By similarity).
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Research Fields
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
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