STON1 Antibody - #AF0704
| Product: | STON1 Antibody |
| Catalog: | AF0704 |
| Description: | Rabbit polyclonal antibody to STON1 |
| Application: | WB IF/ICC |
| Reactivity: | Human |
| Prediction: | Bovine, Chicken |
| Mol.Wt.: | 83kDa; 83kD(Calculated). |
| Uniprot: | Q9Y6Q2 |
| RRID: | AB_2834364 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Prediction:
Bovine(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
STON1 Antibody detects endogenous levels of total STON1.
RRID:
AB_2834364
Cite Format: Affinity Biosciences Cat# AF0704, RRID:AB_2834364.
Cite Format: Affinity Biosciences Cat# AF0704, RRID:AB_2834364.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
DKFZp781K2462; SALF; SBLF; STN1; STNB1; stoned B homolog 1; Stoned B like factor; Stoned b1; Stonin 1;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Description:
Endocytosis of cell surface proteins is mediated by a complex molecular machinery that assembles on the inner surface of the plasma membrane. This gene encodes one of two human homologs of the Drosophila melanogaster stoned B protein. This protein is related to components of the endocytic machinery and exhibits a modular structure consisting of an N-terminal proline-rich domain, a central region of homology specific to the human stoned B-like proteins, and a C-terminal region homologous to the mu subunits of adaptor protein (AP) complexes. Co-transcription of this gene and the neighboring downstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
Sequence:
- Q9Y6Q2 STON1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MCSTNPGKWVTFDDDPAVQSSQKSKNFPLENQGVCRPNGLKLNLPGLREFPSGSSSTSSTPLSSPIVDFYFSPGPPSNSPLSTPTKDFPGFPGIPKAGTHVLYPIPESSSDSPLAISGGESSLLPTRPTCLSHALLPSDHSCTHPTPKVGLPDEVNPQQAESLGFQSDDLPQFQYFREDCAFSSPFWKDEGSDSHFTLDPPGSKKMFSSRNKEMPIDQKSLNKCSLNYICEKLEHLQSAENQDSLRSLSMHCLCAEENASSFVPHTLFRSQPKSGWSFMLRIPEKKNMMSSRQWGPIFLKVLPGGILQMYYEQGLEKPFKEIQLDPYCRLSEPKVENFSVAGKIHTVKIEHVSYTEKRKYHSKTEVVHEPDIEQMLKLGSTSYHDFLDFLTTVEEELMKLPAVSKPKKNYEEQEISLEIVDNFWGKVTKEGKFVESAVITQIYCLCFVNGNLECFLTLNDLELPKRDESYYEKDSEKKGIDILDYHFHKCVNVQEFEQSRIIKFVPLDACRFELMRFKTLYNGDNLPFSLKSVVVVQGAYVELQAFVNMASLAQRSSYAGSLRSCDNIRIHFPVPSQWIKALWTMNLQRQKSLKAKMNRRACLGSLQELESEPVIQVTVGSAKYESAYQAVVWKIDRLPDKNSSLDHPHCLSYKLELGSDQEIPSDWYPFATVQFSVPDTCASRTEVRSLGVESDVQPQKHVQQRACYNIQVEIEKKWIKIDGEDPDKIGDCITQ
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Bovine
100
Chicken
80
Sheep
67
Pig
0
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y6Q2 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K205 | Ubiquitination | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| T346 | Phosphorylation | Uniprot | |
| S404 | Phosphorylation | Uniprot | |
| Y628 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
May be involved in the endocytic machinery.
Subcellular Location:
Cytoplasm. Membrane.
Note: Some fraction is membrane-associated.
Tissue Specificity:
Ubiquitous.
Family&Domains:
Belongs to the Stoned B family.
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For Research Use Only.
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