ZNF148 Antibody - #AF0792
(3)
| Product: | ZNF148 Antibody |
| Catalog: | AF0792 |
| Description: | Rabbit polyclonal antibody to ZNF148 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus |
| Mol.Wt.: | 89kDa; 89kD(Calculated). |
| Uniprot: | Q9UQR1 |
| RRID: | AB_2834348 |
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
ZNF148 Antibody detects endogenous levels of total ZNF148.
RRID:
AB_2834348
Cite Format: Affinity Biosciences Cat# AF0792, RRID:AB_2834348.
Cite Format: Affinity Biosciences Cat# AF0792, RRID:AB_2834348.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Transcription factor ZBP-89; Transcription factor ZBP89; ZBP89; Zinc finger DNA binding protein 89; Zinc finger DNA-binding protein 89; Zinc finger protein 148; ZN148_HUMAN; ZNF 148; ZNF148;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Description:
ZNF148 a transcription factor. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes. Belongs to the krueppel C2H2-type zinc-finger protein family.
Sequence:
- Q9UQR1 ZN148_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNIDDKLEGLFLKCGGIDEMQSSRTMVVMGGVSGQSTVSGELQDSVLQDRSMPHQEILAADEVLQESEMRQQDMISHDELMVHEETVKNDEEQMETHERLPQGLQYALNVPISVKQEITFTDVSEQLMRDKKQIREPVDLQKKKKRKQRSPAKILTINEDGSLGLKTPKSHVCEHCNAAFRTNYHLQRHVFIHTGEKPFQCSQCDMRFIQKYLLQRHEKIHTGEKPFRCDECGMRFIQKYHMERHKRTHSGEKPYQCEYCLQYFSRTDRVLKHKRMCHENHDKKLNRCAIKGGLLTSEEDSGFSTSPKDNSLPKKKRQKTEKKSSGMDKESALDKSDLKKDKNDYLPLYSSSTKVKDEYMVAEYAVEMPHSSVGGSHLEDASGEIHPPKLVLKKINSKRSLKQPLEQNQTISPLSTYEESKVSKYAFELVDKQALLDSEGNADIDQVDNLQEGPSKPVHSSTNYDDAMQFLKKKRYLQAASNNSREYALNVGTIASQPSVTQAAVASVIDESTTASILESQALNVEIKSNHDKNVIPDEVLQTLLDHYSHKANGQHEISFSVADTEVTSSISINSSEVPEVTPSENVGSSSQASSSDKANMLQEYSKFLQQALDRTSQNDAYLNSPSLNFVTDNQTLPNQPAFSSIDKQVYATMPINSFRSGMNSPLRTTPDKSHFGLIVGDSQHSFPFSGDETNHASATSTQDFLDQVTSQKKAEAQPVHQAYQMSSFEQPFRAPYHGSRAGIATQFSTANGQVNLRGPGTSAEFSEFPLVNVNDNRAGMTSSPDATTGQTFG
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Xenopus
80
Chicken
75
Pig
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UQR1 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T25 | Phosphorylation | Uniprot | |
| T37 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| K115 | Sumoylation | Uniprot | |
| K132 | Sumoylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| T167 | Phosphorylation | Uniprot | |
| T194 | Phosphorylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| S202 | Phosphorylation | Q13315 (ATM) | Uniprot |
| T222 | Phosphorylation | Uniprot | |
| K239 | Sumoylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| Y259 | Phosphorylation | Uniprot | |
| K291 | Methylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| T296 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| T305 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| Y345 | Phosphorylation | Uniprot | |
| Y349 | Phosphorylation | Uniprot | |
| K356 | Sumoylation | Uniprot | |
| S400 | Phosphorylation | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| T410 | Phosphorylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| Y417 | Phosphorylation | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| K424 | Sumoylation | Uniprot | |
| K424 | Ubiquitination | Uniprot | |
| Y425 | Phosphorylation | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| K456 | Acetylation | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| K472 | Ubiquitination | Uniprot | |
| K533 | Ubiquitination | Uniprot | |
| K607 | Acetylation | Uniprot | |
| K607 | Ubiquitination | Uniprot | |
| S627 | Phosphorylation | Uniprot | |
| S661 | Phosphorylation | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| T669 | Phosphorylation | Uniprot | |
| T670 | Phosphorylation | Uniprot | |
| R734 | Methylation | Uniprot | |
| S740 | Phosphorylation | Uniprot | |
| S749 | Phosphorylation | Uniprot | |
| S784 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.
PTMs:
Sumoylated with SUMO2. Desumoylated by SENP3, resulting in the stimulation of transcription of its target genes (By similarity).
Subcellular Location:
Nucleus.
Subunit Structure:
Interacts with HNRNPDL. Interacts with the 5FMC complex; the interaction requires association with CHTOP. Interacts with CAVIN1 (By similarity).
Family&Domains:
Belongs to the krueppel C2H2-type zinc-finger protein family.
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