ASPM Antibody - #DF10064

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Product: ASPM Antibody
Catalog: DF10064
Description: Rabbit polyclonal antibody to ASPM
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Bovine, Horse, Sheep, Dog
Mol.Wt.: 410 kDa; 410kD(Calculated).
Uniprot: Q8IZT6
RRID: AB_2840644

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Bovine(82%), Horse(100%), Sheep(82%), Dog(90%)
Clonality:
Polyclonal
Specificity:
ASPM Antibody detects endogenous levels of total ASPM.
RRID:
AB_2840644
Cite Format: Affinity Biosciences Cat# DF10064, RRID:AB_2840644.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:
Uniprot:

Q8IZT6(ASPM_HUMAN) >>Visit HPA database.

Gene(ID):
ASPM(259266)
Sequence:
MANRRVGRGCWEVSPTERRPPAGLRGPAAEEEASSPPVLSLSHFCRSPFLCFGDVLLGASRTLSLALDNPNEEVAEVKISHFPAADLGFSVSQRCFVLQPKEKIVISVNWTPLKEGRVREIMTFLVNDVLKHQAILLGNAEEQKKKKRSLWDTIKKKKISASTSHNRRVSNIQNVNKTFSVSQKVDRVRSPLQACENLAMNEGGPPTENNSLILEENKIPISPISPAFNECHGATCLPLSVRRSTTYSSLHASENRELLNVHSANVSKVSFNEKAVTETSFNSVNVNGQRGENSKLSLTPNCSSTLNITQSQIHFLSPDSFVNNSHGANNELELVTCLSSDMFMKDNSQPVHLESTIAHEIYQKILSPDSFIKDNYGLNQDLESESVNPILSPNQFLKDNMAYMCTSQQTCKVPLSNENSQVPQSPEDWRKSEVSPRIPECQGSKSPKAIFEELVEMKSNYYSFIKQNNPKFSAVQDISSHSHNKQPKRRPILSATVTKRKATCTRENQTEINKPKAKRCLNSAVGEHEKVINNQKEKEDFHSYLPIIDPILSKSKSYKNEVTPSSTTASVARKRKSDGSMEDANVRVAITEHTEVREIKRIHFSPSEPKTSAVKKTKNVTTPISKRISNREKLNLKKKTDLSIFRTPISKTNKRTKPIIAVAQSSLTFIKPLKTDIPRHPMPFAAKNMFYDERWKEKQEQGFTWWLNFILTPDDFTVKTNISEVNAATLLLGIENQHKISVPRAPTKEEMSLRAYTARCRLNRLRRAACRLFTSEKMVKAIKKLEIEIEARRLIVRKDRHLWKDVGERQKVLNWLLSYNPLWLRIGLETTYGELISLEDNSDVTGLAMFILNRLLWNPDIAAEYRHPTVPHLYRDGHEEALSKFTLKKLLLLVCFLDYAKISRLIDHDPCLFCKDAEFKASKEILLAFSRDFLSGEGDLSRHLGLLGLPVNHVQTPFDEFDFAVTNLAVDLQCGVRLVRTMELLTQNWDLSKKLRIPAISRLQKMHNVDIVLQVLKSRGIELSDEHGNTILSKDIVDRHREKTLRLLWKIAFAFQVDISLNLDQLKEEIAFLKHTKSIKKTISLLSCHSDDLINKKKGKRDSGSFEQYSENIKLLMDWVNAVCAFYNKKVENFTVSFSDGRVLCYLIHHYHPCYVPFDAICQRTTQTVECTQTGSVVLNSSSESDDSSLDMSLKAFDHENTSELYKELLENEKKNFHLVRSAVRDLGGIPAMINHSDMSNTIPDEKVVITYLSFLCARLLDLRKEIRAARLIQTTWRKYKLKTDLKRHQEREKAARIIQLAVINFLAKQRLRKRVNAALVIQKYWRRVLAQRKLLMLKKEKLEKVQNKAASLIQGYWRRYSTRQRFLKLKYYSIILQSRIRMIIAVTSYKRYLWATVTIQRHWRAYLRRKQDQQRYEMLKSSTLIIQSMFRKWKQRKMQSQVKATVILQRAFREWHLRKQAKEENSAIIIQSWYRMHKELRKYIYIRSCVVIIQKRFRCFQAQKLYKRRKESILTIQKYYKAYLKGKIERTNYLQKRAAAIQLQAAFRRLKAHNLCRQIRAACVIQSYWRMRQDRVRFLNLKKTIIKFQAHVRKHQQRQKYKKMKKAAVIIQTHFRAYIFAMKVLASYQKTRSAVIVLQSAYRGMQARKMYIHILTSVIKIQSYYRAYVSKKEFLSLKNATIKLQSTVKMKQTRKQYLHLRAAALFIQQCYRSKKIAAQKREEYMQMRESCIKLQAFVRGYLVRKQMRLQRKAVISLQSYFRMRKARQYYLKMYKAIIVIQNYYHAYKAQVNQRKNFLQVKKAATCLQAAYRGYKVRQLIKQQSIAALKIQSAFRGYNKRVKYQSVLQSIIKIQRWYRAYKTLHDTRTHFLKTKAAVISLQSAYRGWKVRKQIRREHQAALKIQSAFRMAKAQKQFRLFKTAALVIQQNFRAWTAGRKQCMEYIELRHAVLVLQSMWKGKTLRRQLQRQHKCAIIIQSYYRMHVQQKKWKIMKKAALLIQKYYRAYSIGREQNHLYLKTKAAVVTLQSAYRGMKVRKRIKDCNKAAVTIQSKYRAYKTKKKYATYRASAIIIQRWYRGIKITNHQHKEYLNLKKTAIKIQSVYRGIRVRRHIQHMHRAATFIKAMFKMHQSRISYHTMRKAAIVIQVRCRAYYQGKMQREKYLTILKAVKVLQASFRGVRVRRTLRKMQTAATLIQSNYRRYRQQTYFNKLKKITKTVQQRYWAMKERNIQFQRYNKLRHSVIYIQAIFRGKKARRHLKMMHIAATLIQRRFRTLMMRRRFLSLKKTAILIQRKYRAHLCTKHHLQFLQVQNAVIKIQSSYRRWMIRKRMREMHRAATFIQSTFRMHRLHMRYQALKQASVVIQQQYQANRAAKLQRQHYLRQRHSAVILQAAFRGMKTRRHLKSMHSSATLIQSRFRSLLVRRRFISLKKATIFVQRKYRATICAKHKLYQFLHLRKAAITIQSSYRRLMVKKKLQEMQRAAVLIQATFRMYRTYITFQTWKHASILIQQHYRTYRAAKLQRENYIRQWHSAVVIQAAYKGMKARQLLREKHKASIVIQSTYRMYRQYCFYQKLQWATKIIQEKYRANKKKQKVFQHNELKKETCVQAGFQDMNIKKQIQEQHQAAIIIQKHCKAFKIRKHYLHLRATVVSIQRRYRKLTAVRTQAVICIQSYYRGFKVRKDIQNMHRAATLIQSFYRMHRAKVDYETKKTAIVVIQNYYRLYVRVKTERKNFLAVQKSVRTIQAAFRGMKVRQKLKNVSEEKMAAIVNQSALCCYRSKTQYEAVQSEGVMIQEWYKASGLACSQEAEYHSQSRAAVTIQKAFCRMVTRKLETQKCAALRIQFFLQMAVYRRRFVQQKRAAITLQHYFRTWQTRKQFLLYRKAAVVLQNHYRAFLSAKHQRQVYLQIRSSVIIIQARSKGFIQKRKFQEIKNSTIKIQAMWRRYRAKKYLCKVKAACKIQAWYRCWRAHKEYLAILKAVKIIQGCFYTKLERTRFLNVRASAIIIQRKWRAILPAKIAHEHFLMIKRHRAACLIQAHYRGYKGRQVFLRQKSAALIIQKYIRAREAGKHERIKYIEFKKSTVILQALVRGWLVRKRFLEQRAKIRLLHFTAAAYYHLNAVRIQRAYKLYLAVKNANKQVNSVICIQRWFRARLQEKRFIQKYHSIKKIEHEGQECLSQRNRAASVIQKAVRHFLLRKKQEKFTSGIIKIQALWRGYSWRKKNDCTKIKAIRLSLQVVNREIREENKLYKRTALALHYLLTYKHLSAILEALKHLEVVTRLSPLCCENMAQSGAISKIFVLIRSCNRSIPCMEVIRYAVQVLLNVSKYEKTTSAVYDVENCIDILLELLQIYREKPGNKVADKGGSIFTKTCCLLAILLKTTNRASDVRSRSKVVDRIYSLYKLTAHKHKMNTERILYKQKKNSSISIPFIPETPVRTRIVSRLKPDWVLRRDNMEEITNPLQAIQMVMDTLGIPY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
90
Bovine
82
Sheep
82
Rabbit
75
Pig
73
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IZT6 As Substrate

Site PTM Type Enzyme
R8 Methylation
S14 Phosphorylation
S35 Phosphorylation
K101 Ubiquitination
T111 Phosphorylation
K144 Sumoylation
K144 Ubiquitination
T153 Phosphorylation
S170 Phosphorylation
K177 Ubiquitination
T178 Phosphorylation
S180 Phosphorylation
K184 Sumoylation
K184 Ubiquitination
S190 Phosphorylation
S211 Phosphorylation
S222 Phosphorylation
S225 Phosphorylation
T245 Phosphorylation
T246 Phosphorylation
Y247 Phosphorylation
S249 Phosphorylation
S263 Phosphorylation
S267 Phosphorylation
S270 Phosphorylation
K274 Ubiquitination
T279 Phosphorylation
S280 Phosphorylation
S283 Phosphorylation
S297 Phosphorylation
S348 Phosphorylation
S355 Phosphorylation
Y362 Phosphorylation
K364 Ubiquitination
S367 Phosphorylation
S370 Phosphorylation
K373 Ubiquitination
S392 Phosphorylation
K398 Ubiquitination
S407 Phosphorylation
S416 Phosphorylation
S420 Phosphorylation
S425 Phosphorylation
K431 Ubiquitination
S432 Phosphorylation
S435 Phosphorylation
S444 Phosphorylation
K445 Ubiquitination
S446 Phosphorylation
Y461 Phosphorylation
Y462 Phosphorylation
S463 Phosphorylation
K466 Ubiquitination
K471 Ubiquitination
S473 Phosphorylation
S482 Phosphorylation
K485 Ubiquitination
S494 Phosphorylation
T496 Phosphorylation
K514 Ubiquitination
K530 Acetylation
K530 Ubiquitination
K538 Ubiquitination
S553 Phosphorylation
K554 Ubiquitination
K559 Sumoylation
K559 Ubiquitination
T563 Phosphorylation
S565 Phosphorylation
S570 Phosphorylation
S577 Phosphorylation
S580 Phosphorylation
S605 Phosphorylation
S607 Phosphorylation
K610 Ubiquitination
T611 Phosphorylation
S612 Phosphorylation
K615 Acetylation
K616 Acetylation
K618 Ubiquitination
T621 Phosphorylation
T622 Phosphorylation
S625 Phosphorylation
K626 Ubiquitination
K639 Ubiquitination
T640 Phosphorylation
S643 Phosphorylation
T647 Phosphorylation
S650 Phosphorylation
K651 Ubiquitination
K674 Ubiquitination
K687 Ubiquitination
K696 Ubiquitination
T729 Phosphorylation
K739 Ubiquitination
T747 Phosphorylation
K748 Ubiquitination
S752 Phosphorylation
K777 Ubiquitination
K804 Ubiquitination
K915 Ubiquitination
K923 Ubiquitination
K1017 Ubiquitination
S1018 Phosphorylation
S1024 Phosphorylation
T1030 Phosphorylation
K1034 Ubiquitination
K1074 Ubiquitination
K1081 Ubiquitination
S1084 Phosphorylation
S1090 Phosphorylation
K1096 Ubiquitination
R1101 Methylation
S1103 Phosphorylation
S1105 Phosphorylation
S1110 Phosphorylation
K1207 Ubiquitination
K1215 Ubiquitination
K1279 Acetylation
Y1325 Phosphorylation
K1334 Ubiquitination
K1339 Acetylation
K1340 Acetylation
K1345 Ubiquitination
K1349 Ubiquitination
Y1361 Phosphorylation
Y1417 Phosphorylation
Y1484 Phosphorylation
K1511 Ubiquitination
S1513 Phosphorylation
T1516 Phosphorylation
K1519 Ubiquitination
K1522 Ubiquitination
K1537 Ubiquitination
K1552 Ubiquitination
S1641 Phosphorylation
Y1643 Phosphorylation
S1658 Phosphorylation
K1679 Ubiquitination
K1684 Ubiquitination
K1696 Ubiquitination
Y1770 Phosphorylation
K1796 Ubiquitination
K1802 Ubiquitination
K1803 Ubiquitination
K1822 Ubiquitination
S1825 Phosphorylation
K1830 Ubiquitination
S1833 Phosphorylation
K1843 Ubiquitination
Y1844 Phosphorylation
S1846 Phosphorylation
S1850 Phosphorylation
K1862 Ubiquitination
K1873 Ubiquitination
K1875 Ubiquitination
K1903 Acetylation
K1903 Ubiquitination
S1906 Phosphorylation
K1912 Acetylation
K1915 Acetylation
K1939 Ubiquitination
Y1944 Phosphorylation
K1972 Ubiquitination
K1995 Ubiquitination
K2002 Ubiquitination
K2021 Ubiquitination
S2029 Phosphorylation
K2045 Ubiquitination
K2053 Acetylation
K2053 Ubiquitination
Y2063 Phosphorylation
S2069 Phosphorylation
K2088 Ubiquitination
K2094 Ubiquitination
T2096 Phosphorylation
K2099 Ubiquitination
S2102 Phosphorylation
Y2104 Phosphorylation
T2121 Phosphorylation
K2124 Ubiquitination
S2132 Phosphorylation
S2135 Phosphorylation
Y2136 Phosphorylation
T2138 Phosphorylation
K2157 Ubiquitination
K2162 Ubiquitination
K2168 Ubiquitination
K2171 Ubiquitination
S2176 Phosphorylation
K2188 Ubiquitination
T2191 Phosphorylation
K2217 Ubiquitination
K2227 Ubiquitination
K2260 Methylation
K2287 Ubiquitination
K2295 Acetylation
K2358 Ubiquitination
K2405 Ubiquitination
S2429 Phosphorylation
K2432 Ubiquitination
K2459 Ubiquitination
T2490 Phosphorylation
S2557 Phosphorylation
Y2570 Phosphorylation
Y2573 Phosphorylation
K2581 Ubiquitination
K2586 Ubiquitination
K2595 Ubiquitination
K2603 Ubiquitination
K2604 Ubiquitination
K2618 Ubiquitination
K2619 Ubiquitination
K2633 Ubiquitination
Y2675 Phosphorylation
Y2676 Phosphorylation
T2730 Phosphorylation
K2733 Ubiquitination
K2740 Ubiquitination
K2759 Ubiquitination
K2765 Ubiquitination
K2781 Ubiquitination
S2806 Phosphorylation
K2823 Ubiquitination
K2837 Ubiquitination
K2877 Ubiquitination
K2884 Ubiquitination
S2912 Phosphorylation
K2933 Ubiquitination
K2960 Ubiquitination
Y2974 Phosphorylation
K2991 Ubiquitination
S3003 Phosphorylation
K3018 Ubiquitination
K3028 Ubiquitination
K3053 Ubiquitination
S3054 Phosphorylation
K3061 Ubiquitination
S3082 Phosphorylation
K3135 Ubiquitination
K3139 Ubiquitination
K3163 Ubiquitination
K3169 Ubiquitination
S3186 Phosphorylation
K3190 Ubiquitination
K3203 Ubiquitination
S3235 Phosphorylation
K3364 Ubiquitination
S3387 Phosphorylation
Y3400 Phosphorylation
K3404 Ubiquitination
T3406 Phosphorylation
K3420 Acetylation
K3423 Acetylation
K3423 Ubiquitination
S3425 Phosphorylation
T3435 Phosphorylation
T3439 Phosphorylation
S3443 Phosphorylation
K3446 Ubiquitination
Y3477 Phosphorylation

Research Backgrounds

Function:

Involved in mitotic spindle regulation and coordination of mitotic processes. The function in regulating microtubule dynamics at spindle poles including spindle orientation, astral microtubule density and poleward microtubule flux seems to depend on the association with the katanin complex formed by KATNA1 and KATNB1. Enhances the microtubule lattice severing activity of KATNA1 by recruiting the katanin complex to microtubules. Can block microtubule minus-end growth and reversely this function can be enhanced by the katanin complex. May have a preferential role in regulating neurogenesis.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton>Spindle. Nucleus.
Note: The nuclear-cytoplasmic distribution could be regulated by the availability of calmodulin (By similarity). Localizes to spindle poles during mitosis (PubMed:19690332). Associates with microtubule minus ends (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with KATNA1 and KATNB1; katanin complex formation KATNA1:KATNB1 is required for the association.

References

1). ASPM Is a Prognostic Biomarker and Correlates With Immune Infiltration in Kidney Renal Clear Cell Carcinoma and Liver Hepatocellular Carcinoma. Frontiers in Oncology, 2022 (PubMed: 35515103) [IF=3.5]

Application: IHC    Species: Human    Sample: KIRC and LIHC tissues

Figure 7 Expression analysis of ASPM in KIRC and LIHC tissues. (A) Relative level of ASPM mRNA using quantitative RT-PCR; **p < 0.01. (B) The expression of ASPM was analyzed by Western-blot analysis using a compound samples. (C) Tumor infiltration of B cells, CD8+ T cells, and M2 macrophages in KIRC and LIHC using immunohistochemistry. 20 diagnosed cases of KIRC and 20 diagnosed cases of LIHC samples for immunohistochemistry. We validate the relationship between ASPM expression and B cells (marker: CD19), CD8+ T cells (marker: CD8A), and M2 macrophages (marker: CD163), we performed immunohistochemistry to assess ASPM, CD19, CD8A, and CD163. Muscle and lymph nodes as control samples. (a-d, f-i) Tumor infiltration of B cells, CD8+ T cells, and M2 macrophages in KIRC. (k-n, p-s) Tumor infiltration of B cells, CD8+ T cells, and M2 macrophages in LIHC. (a-d) High expression of ASPM (+++), CD8A (++), CD19 (++), and CD163 (++) in KIRC. (f-i) Low expression of ASPM (+), CD8A (+), CD19 (+), and CD163 (+) in KIRC. (k-n) High expression of ASPM (+++), CD8A (++), CD19 (++), and CD163 (++) in LIHC. (p-s) Low expression of ASPM (+), CD8A (+), CD19 (+), and CD163 (+) in LIHC. (e, o) lymph nodes was used as a positive control in KIRC (+++) and LIHC (+++). (j, t) muscle was used as a negative control in KIRC (-) and LIHC (-). The expression density of ASPM, CD8A, CD19, and CD163 in KIRC and LIHC tissues were quantitated by scoring staining intensity, including negative (–) and weak (+) staining, moderate (++) and strong (+ + +) staining, respectively.
2). Construction of lncRNA- and circRNA-associated ceRNA networks in the prostatic urethra of rats after simulating transurethral laser prostatectomy (TULP). Molecular and Cellular Biochemistry, 2023 [IF=3.5]

Application: IHC    Species: Rat    Sample:

Fig. 8 The top ten hub genes in the PPI network constructed from DEmRNAs in lncRNA-miRNA-mRNA network (A) and circRNA-miRNA-mRNA (B) were identified; C Immunohistochemistry of ASPM of wound tissues after prostatectomy in rats (left: normal, middle: 5 days, right: 9 days). Black arrows: urothelial cells, blue arrows: prostate ductal epithelial cells

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