Phospho-TALIN1 (Ser425) Antibody - #AF8515

(1)  
Product: Phospho-TALIN1 (Ser425) Antibody
Catalog: AF8515
Description: Rabbit polyclonal antibody to Phospho-TALIN1 (Ser425)
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 270 kDa; 270kD(Calculated).
Uniprot: Q9Y490
RRID: AB_2840569

View similar products>>

   Size Price Inventory
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-TALIN1 (Ser425) Antibody detects endogenous levels of TALIN1 only when phosphorylated at Ser425.
RRID:
AB_2840569
Cite Format: Affinity Biosciences Cat# AF8515, RRID:AB_2840569.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ILWEQ; Talin 1; Talin; Talin-1; TLN 1; TLN; Tln1; TLN1_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q9Y490(TLN1_HUMAN) >>Visit HPA database.

Gene(ID):
TLN1(7094)
Sequence:
MVALSLKISIGNVVKTMQFEPSTMVYDACRIIRERIPEAPAGPPSDFGLFLSDDDPKKGIWLEAGKALDYYMLRNGDTMEYRKKQRPLKIRMLDGTVKTIMVDDSKTVTDMLMTICARIGITNHDEYSLVRELMEEKKEEITGTLRKDKTLLRDEKKMEKLKQKLHTDDELNWLDHGRTLREQGVEEHETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFAGFQCQIQFGPHNEQKHKAGFLDLKDFLPKEYVKQKGERKIFQAHKNCGQMSEIEAKVRYVKLARSLKTYGVSFFLVKEKMKGKNKLVPRLLGITKECVMRVDEKTKEVIQEWNLTNIKRWAASPKSFTLDFGDYQDGYYSVQTTEGEQIAQLIAGYIDIILKKKKSKDHFGLEGDEESTMLEDSVSPKKSTVLQQQYNRVGKVEHGSVALPAIMRSGASGPENFQVGSMPPAQQQITSGQMHRGHMPPLTSAQQALTGTINSSMQAVQAAQATLDDFDTLPPLGQDAASKAWRKNKMDESKHEIHSQVDAITAGTASVVNLTAGDPAETDYTAVGCAVTTISSNLTEMSRGVKLLAALLEDEGGSGRPLLQAAKGLAGAVSELLRSAQPASAEPRQNLLQAAGNVGQASGELLQQIGESDTDPHFQDALMQLAKAVASAAAALVLKAKSVAQRTEDSGLQTQVIAAATQCALSTSQLVACTKVVAPTISSPVCQEQLVEAGRLVAKAVEGCVSASQAATEDGQLLRGVGAAATAVTQALNELLQHVKAHATGAGPAGRYDQATDTILTVTENIFSSMGDAGEMVRQARILAQATSDLVNAIKADAEGESDLENSRKLLSAAKILADATAKMVEAAKGAAAHPDSEEQQQRLREAAEGLRMATNAAAQNAIKKKLVQRLEHAAKQAAASATQTIAAAQHAASTPKASAGPQPLLVQSCKAVAEQIPLLVQGVRGSQAQPDSPSAQLALIAASQSFLQPGGKMVAAAKASVPTIQDQASAMQLSQCAKNLGTALAELRTAAQKAQEACGPLEMDSALSVVQNLEKDLQEVKAAARDGKLKPLPGETMEKCTQDLGNSTKAVSSAIAQLLGEVAQGNENYAGIAARDVAGGLRSLAQAARGVAALTSDPAVQAIVLDTASDVLDKASSLIEEAKKAAGHPGDPESQQRLAQVAKAVTQALNRCVSCLPGQRDVDNALRAVGDASKRLLSDSLPPSTGTFQEAQSRLNEAAAGLNQAATELVQASRGTPQDLARASGRFGQDFSTFLEAGVEMAGQAPSQEDRAQVVSNLKGISMSSSKLLLAAKALSTDPAAPNLKSQLAAAARAVTDSINQLITMCTQQAPGQKECDNALRELETVRELLENPVQPINDMSYFGCLDSVMENSKVLGEAMTGISQNAKNGNLPEFGDAISTASKALCGFTEAAAQAAYLVGVSDPNSQAGQQGLVEPTQFARANQAIQMACQSLGEPGCTQAQVLSAATIVAKHTSALCNSCRLASARTTNPTAKRQFVQSAKEVANSTANLVKTIKALDGAFTEENRAQCRAATAPLLEAVDNLSAFASNPEFSSIPAQISPEGRAAMEPIVISAKTMLESAGGLIQTARALAVNPRDPPSWSVLAGHSRTVSDSIKKLITSMRDKAPGQLECETAIAALNSCLRDLDQASLAAVSQQLAPREGISQEALHTQMLTAVQEISHLIEPLANAARAEASQLGHKVSQMAQYFEPLTLAAVGAASKTLSHPQQMALLDQTKTLAESALQLLYTAKEAGGNPKQAAHTQEALEEAVQMMTEAVEDLTTTLNEAASAAGVVGGMVDSITQAINQLDEGPMGEPEGSFVDYQTTMVRTAKAIAVTVQEMVTKSNTSPEELGPLANQLTSDYGRLASEAKPAAVAAENEEIGSHIKHRVQELGHGCAALVTKAGALQCSPSDAYTKKELIECARRVSEKVSHVLAALQAGNRGTQACITAASAVSGIIADLDTTIMFATAGTLNREGTETFADHREGILKTAKVLVEDTKVLVQNAAGSQEKLAQAAQSSVATITRLADVVKLGAASLGAEDPETQVVLINAVKDVAKALGDLISATKAAAGKVGDDPAVWQLKNSAKVMVTNVTSLLKTVKAVEDEATKGTRALEATTEHIRQELAVFCSPEPPAKTSTPEDFIRMTKGITMATAKAVAAGNSCRQEDVIATANLSRRAIADMLRACKEAAYHPEVAPDVRLRALHYGRECANGYLELLDHVLLTLQKPSPELKQQLTGHSKRVAGSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANALDDGQWSQGLISAARMVAAATNNLCEAANAAVQGHASQEKLISSAKQVAASTAQLLVACKVKADQDSEAMKRLQAAGNAVKRASDNLVKAAQKAAAFEEQENETVVVKEKMVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELRDEH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Bovine
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y490 As Substrate

Site PTM Type Enzyme
T16 Phosphorylation
S22 Phosphorylation
T23 Phosphorylation
Y26 Phosphorylation
C29 S-Nitrosylation
R35 Methylation
S45 Phosphorylation
K57 Acetylation
Y70 Phosphorylation
Y71 Phosphorylation
R74 Methylation
T78 Phosphorylation
Y81 Phosphorylation
T99 Phosphorylation
S105 Phosphorylation
T114 Phosphorylation
T122 Phosphorylation
Y127 Phosphorylation
S128 Phosphorylation
K138 Ubiquitination
T142 Phosphorylation
T144 Phosphorylation
R146 Methylation
K149 Ubiquitination
T150 Phosphorylation
K164 Ubiquitination
T167 Phosphorylation
Y199 Phosphorylation
K254 Ubiquitination
K256 Ubiquitination
K268 Ubiquitination
Y270 Phosphorylation
K284 Ubiquitination
C286 S-Nitrosylation
K295 Ubiquitination
K334 Ubiquitination
T354 Phosphorylation
K357 Ubiquitination
S405 Phosphorylation
K406 Ubiquitination
S423 Phosphorylation
S425 Phosphorylation Q00535 (CDK5)
K428 Methylation
K428 Ubiquitination
S429 Phosphorylation
Y436 Phosphorylation
K441 Ubiquitination
S446 Phosphorylation
R482 Methylation
T568 Phosphorylation
Y570 Phosphorylation
T571 Phosphorylation
S604 Phosphorylation
S620 Phosphorylation
S625 Phosphorylation
S677 Phosphorylation
S729 Phosphorylation
S834 Phosphorylation
S858 Phosphorylation
K861 Ubiquitination
K869 Acetylation
K869 Ubiquitination
K875 Ubiquitination
R889 Methylation
R898 Methylation
T931 Phosphorylation
T941 Phosphorylation
S945 Phosphorylation
S955 Phosphorylation
S973 Phosphorylation
S979 Phosphorylation
S981 Phosphorylation
S992 Phosphorylation
T1010 Phosphorylation
S1016 Phosphorylation
S1021 Phosphorylation
T1029 Phosphorylation
R1035 Methylation
S1055 Phosphorylation
Y1116 Phosphorylation
S1130 Phosphorylation
T1142 Phosphorylation
S1163 Phosphorylation
S1164 Phosphorylation
S1201 Phosphorylation
S1225 Phosphorylation
S1227 Phosphorylation
S1260 Phosphorylation
T1263 Phosphorylation
S1271 Phosphorylation
S1311 Phosphorylation
S1323 Phosphorylation
T1324 Phosphorylation
T1343 Phosphorylation
S1411 Phosphorylation
Y1445 Phosphorylation
S1508 Phosphorylation
S1528 Phosphorylation
S1535 Phosphorylation
K1541 Acetylation
K1541 Ubiquitination
K1544 Acetylation
K1544 Ubiquitination
S1589 Phosphorylation
T1616 Phosphorylation
S1641 Phosphorylation
S1643 Phosphorylation
S1670 Phosphorylation
S1679 Phosphorylation
S1732 Phosphorylation
Y1737 Phosphorylation
S1750 Phosphorylation
T1767 Phosphorylation
Y1777 Phosphorylation
S1849 Phosphorylation
T1855 Phosphorylation
Y1893 Phosphorylation
S1898 Phosphorylation
S1914 Phosphorylation
K1917 Ubiquitination
C1939 S-Nitrosylation
S1940 Phosphorylation
Y1945 Phosphorylation
T1946 Phosphorylation
K1947 Acetylation
K1948 Ubiquitination
K1960 Ubiquitination
S1962 Phosphorylation
T2009 Phosphorylation
K2021 Ubiquitination
K2024 Acetylation
T2030 Phosphorylation
K2031 Acetylation
S2040 Phosphorylation
S2068 Phosphorylation
K2089 Acetylation
S2096 Phosphorylation
T2098 Phosphorylation
K2115 Acetylation
K2115 Methylation
K2115 Ubiquitination
T2123 Phosphorylation
S2127 Phosphorylation
K2133 Acetylation
S2162 Phosphorylation
T2171 Phosphorylation
K2180 Ubiquitination
T2183 Phosphorylation
T2186 Phosphorylation
R2197 Methylation
Y2224 Phosphorylation
R2233 Methylation
Y2239 Phosphorylation
S2262 Phosphorylation
T2270 Phosphorylation
T2281 Phosphorylation
S2338 Phosphorylation
S2351 Phosphorylation
S2357 Phosphorylation
K2361 Ubiquitination
S2390 Phosphorylation
T2404 Phosphorylation
S2426 Phosphorylation
S2427 Phosphorylation
Y2530 Phosphorylation
K2531 Ubiquitination
S2535 Phosphorylation
R2538 Methylation

Research Backgrounds

Function:

Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts (By similarity).

Subcellular Location:

Cell projection>Ruffle membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cytoskeleton. Cell surface. Cell junction>Focal adhesion.
Note: Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds with high affinity to VCL and with low affinity to integrins. Interacts with APBB1IP; this inhibits VCL binding. Interacts with PTK2/FAK1 (By similarity). Interacts with PIP5K1C and NRAP. Interacts with LAYN (By similarity). Interacts with SYNM. Interacts with ITGB1; the interaction is prevented by competitive binding of ITGB1BP1.

(Microbial infection) Interacts with human cytomegalovirus protein UL135.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.