Phospho-PDHA1/2 (Ser293/Ser291) Antibody - #AF8502

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Product: Phospho-PDHA1/2 (Ser293/Ser291) Antibody
Catalog: AF8502
Description: Rabbit polyclonal antibody to Phospho-PDHA1/2 (Ser293/Ser291)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 43 kDa; 43kD(Calculated).
Uniprot: P08559 | P29803
RRID: AB_2840556

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 100ul $350 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-PDHA1/2 (Ser293/Ser291) Antibody detects endogenous levels of PDHA1/2 only when phosphorylated at Ser293/291.
RRID:
AB_2840556
Cite Format: Affinity Biosciences Cat# AF8502, RRID:AB_2840556.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ODPA_HUMAN; PDH; PDHA; PDHA1; PDHCE1A; PDHE1 A type I; PDHE1-A type I; PHE1A; Pyruvate Dehydrogenase (lipoamide) alpha 1; Pyruvate dehydrogenase complex, E1 alpha polypeptide 1; Pyruvate Dehydrogenase E1 alpha; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; EC 1.2.4.1; MGC149517; MGC149518; mitochondrial; ODPAT_HUMAN; Pdha2; PDHAL; PDHE1 A type II; PDHE1-A type II; Pyruvate dehydrogenase (lipoamide) alpha 2; Pyruvate dehydrogenase E1 component subunit alpha; Pyruvate dehydrogenase E1 component subunit alpha, testis specific form, mitochondrial; Pyruvate dehydrogenase, E1 alpha polypeptide, testis spec; testis-specific form;

Immunogens

Immunogen:
Uniprot:

P08559(ODPA_HUMAN) >>Visit HPA database.

P29803(ODPAT_HUMAN) >>Visit HPA database.

Gene(ID):
PDHA1(5160) | PDHA2(5161)
Expression:
P08559 ODPA_HUMAN:

Ubiquitous.

P29803 ODPAT_HUMAN:

Testis. Expressed in postmeiotic spermatogenic cells.

Sequence:
MRKMLAAVSRVLSGASQKPASRVLVASRNFANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS

MLAAFISRVLRRVAQKSARRVLVASRNSSNDATFEIKKCDLYLLEEGPPVTTVLTRAEGLKYYRMMLTVRRMELKADQLYKQKFIRGFCHLCDGQEACCVGLEAGINPSDHVITSYRAHGVCYTRGLSVRSILAELTGRRGGCAKGKGGSMHMYTKNFYGGNGIVGAQGPLGAGIALACKYKGNDEICLTLYGDGAANQGQIAEAFNMAALWKLPCVFICENNLYGMGTSTERAAASPDYYKRGNFIPGLKVDGMDVLCVREATKFAANYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKRDPIIILQDRMVNSKLATVEELKEIGAEVRKEIDDAAQFATTDPEPHLEELGHHIYSSDSSFEVRGANPWIKFKSVS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Rabbit
100
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08559/P29803 As Substrate

Site PTM Type Enzyme
K18 Acetylation
K18 Ubiquitination
K39 Acetylation
K39 Ubiquitination
K63 Acetylation
K63 Ubiquitination
K77 Acetylation
K83 Acetylation
K83 Ubiquitination
T116 Phosphorylation
Y118 Phosphorylation
T139 Phosphorylation
S152 Phosphorylation
Y227 Phosphorylation
T231 Phosphorylation
S232 Phosphorylation Q15118 (PDK1) , O15530 (PDPK1) , Q15119 (PDK2)
T240 Phosphorylation
Y242 Phosphorylation
Y243 Phosphorylation
K244 Acetylation
K244 Ubiquitination
R245 Methylation
Y272 Phosphorylation
K277 Acetylation
Y289 Phosphorylation
S293 Phosphorylation Q16654 (PDK4) , Q15119 (PDK2) , O15530 (PDPK1) , Q15120 (PDK3) , Q15118 (PDK1)
S295 Phosphorylation
S300 Phosphorylation Q15119 (PDK2) , Q15118 (PDK1) , Q16654 (PDK4) , Q15120 (PDK3) , O15530 (PDPK1)
Y301 Phosphorylation
T303 Phosphorylation
K313 Acetylation
K321 Acetylation
K321 Ubiquitination
K336 Acetylation
K344 Ubiquitination
Y366 Phosphorylation
Y369 Phosphorylation
K385 Methylation
Site PTM Type Enzyme
K16 Sumoylation
K37 Acetylation
Y42 Phosphorylation
Y62 Phosphorylation
Y63 Phosphorylation
K75 Acetylation
K81 Acetylation
K81 Ubiquitination
S150 Phosphorylation
Y154 Phosphorylation
S230 Phosphorylation Q15118 (PDK1)
K275 Acetylation
Y287 Phosphorylation
S291 Phosphorylation Q15120 (PDK3) , Q15119 (PDK2) , Q15118 (PDK1) , Q16654 (PDK4)
S293 Phosphorylation
S298 Phosphorylation Q16654 (PDK4) , Q15119 (PDK2) , Q15120 (PDK3) , Q15118 (PDK1)
Y299 Phosphorylation
T301 Phosphorylation

Research Backgrounds

Function:

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

PTMs:

Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation.

Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3 (By similarity).

Subcellular Location:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Heterotetramer of two PDHA1 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules.

Function:

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

PTMs:

Phosphorylation at Ser-291, Ser-293 and Ser-298 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Phosphorylation at Ser-293 interferes with access to active site, and thereby inactivates the enzyme. Dephosphorylation at all three sites, i.e. at Ser-291, Ser-293 and Ser-298, is required for reactivation.

Subcellular Location:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Testis. Expressed in postmeiotic spermatogenic cells.

Subunit Structure:

Heterotetramer of two PDHA2 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

References

1). HIF-1 regulates energy metabolism of the Tibetan chicken brain during embryo development under hypoxia. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (PubMed: 33596720) [IF=2.8]

Application: WB    Species: chicken    Sample:

Figure 5. Protein expression results of related genes during hypoxia at different developmental stages of the Tibetan chicken (TBC) and Dwarf Laying Chicken (DLC) embryonic brain. A–C: Western blot results of related genes in TBCs (A and B) and DLCs (C) under hypoxia as the embryo develops. D: chromatin immunoprecipitation (ChIP) assay of hypoxia-inducible factor-1 (HIF-1) binding to hexokinase 1 (HK1) and lactate dehydrogenase A (LDHA) was performed on the chicken embryonic brain. HTBC, TBCs under hypoxia; HDLC, DLCs under hypoxia. Data are presented as means ± SE.

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