GLUT3 Antibody | Affinity Biosciences

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Western blot analysis on LOVO cell lysates using GLUT3 Antibody,The lane on the left was treated with the antigen-specific peptide.

Product:	GLUT3 Antibody
Catalog:	AF0174
Description:	Rabbit polyclonal antibody to GLUT3
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Horse
Mol.Wt.:	54kDa; 54kD(Calculated).
Uniprot:	P11169
RRID:	AB_2833367

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Horse(88%)

Clonality:

Polyclonal

Specificity:

GLUT3 Antibody detects endogenous levels of total GLUT3.

RRID:

AB_2833367
Cite Format: Affinity Biosciences Cat# AF0174, RRID:AB_2833367.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

brain; FLJ90380; Glucose transporter type 3; Glucose transporter type 3 brain; GLUT 3; GLUT-3; GLUT3; GTR3_HUMAN; Slc2a3; Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3; Solute carrier family 2, facilitated glucose transporter member 3;

Immunogens

Immunogen:

Uniprot:

P11169(GTR3_HUMAN) >>Visit HPA database.

Gene(ID):

SLC2A3(6515)

Expression:

P11169 GTR3_HUMAN:

Highly expressed in brain. Expressed in many tissues.

Description:

GLUT3 an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. It s surface expression is induced by insulin. It is restricted in humans to brain, testis (in the spermatozoa), and skeletal muscle, mostly in triads of slow-twitch fibres. Detected only in the brain of mouse and rat.

Sequence:

P11169 GTR3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGTQKVTPALIFAITVATIGSFQFGYNTGVINAPEKIIKEFINKTLTDKGNAPPSEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLLAVTGGCFMGLCKVAKSVEMLILGRLVIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVVGILVAQIFGLEFILGSEELWPLLLGFTILPAILQSAALPFCPESPRFLLINRKEEENAKQILQRLWGTQDVSQDIQEMKDESARMSQEKQVTVLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFKDAGVQEPIYATIGAGVVNTIFTVVSLFLVERAGRRTLHMIGLGGMAFCSTLMTVSLLLKDNYNGMSFVCIGAILVFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAHYLGAYVFIIFTGFLITFLAFTFFKVPETRGRTFEDITRAFEGQAHGADRSGKDGVMEMNSIEPAKETTTNV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Pig

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11169 As Substrate

P11169

Site	PTM Type	Source
K223	Ubiquitination	Uniprot
T232	Phosphorylation	Uniprot
K243	Ubiquitination	Uniprot
S250	Phosphorylation	Uniprot
K253	Ubiquitination	Uniprot
T333	Phosphorylation	Uniprot
S352	Phosphorylation	Uniprot
K477	Ubiquitination	Uniprot
S485	Phosphorylation	Uniprot
K490	Ubiquitination	Uniprot

Research Backgrounds

P11169_GTR3_HUMAN

Function:

Facilitative glucose transporter that can also mediate the uptake of various other monosaccharides across the cell membrane. Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate. Does not mediate fructose transport.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Perikaryon. Cell projection.
Note: Localized to densely spaced patches along neuronal processes.

Tissue Specificity:

Highly expressed in brain. Expressed in many tissues.

Family&Domains:

Transport is mediated via a series of conformation changes, switching between a conformation where the substrate-binding cavity is accessible from the outside, and a another conformation where it is accessible from the cytoplasm.

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

References

1). Simulated microgravity led to increased brown adipose tissue activity in rats. Acta Astronautica, 2019 [IF=3.5]

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GLUT3 Antibody - #AF0174