Phospho-BCL-3 (Ser402) Antibody - #AF8466

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Product: Phospho-BCL-3 (Ser402) Antibody
Catalog: AF8466
Description: Rabbit polyclonal antibody to Phospho-BCL-3 (Ser402)
Application: WB IHC
Reactivity: Human
Prediction: Horse
Mol.Wt.: 60kD; 48kD(Calculated).
Uniprot: P20749
RRID: AB_2840520

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 200ul $450 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Horse(100%)
Clonality:
Polyclonal
Specificity:
Phospho-BCL-3 (Ser402) Antibody detects endogenous levels of BCL-3 only when phosphorylated at Ser402.
RRID:
AB_2840520
Cite Format: Affinity Biosciences Cat# AF8466, RRID:AB_2840520.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B cell CLL/lymphoma 3; B cell leukemia/lymphoma 3; B cell lymphoma 3 encoded protein; B-cell lymphoma 3 protein; Bcl 3; BCL 4; BCL-3; Bcl3; BCL3_HUMAN; BCL4; Chronic lymphatic leukemia protein; D19S37; Protein Bcl 3; Proto-oncogene BCL3;

Immunogens

Immunogen:
Uniprot:

P20749(BCL3_HUMAN) >>Visit HPA database.

Gene(ID):
BCL3(602)
Sequence:
MPRCPAGAMDEGPVDLRTRPKAAGLPGAALPLRKRPLRAPSPEPAAPRGAAGLVVPLDPLRGGCDLPAVPGPPHGLARPEALYYPGALLPLYPTRAMGSPFPLVNLPTPLYPMMCPMEHPLSADIAMATRADEDGDTPLHIAVVQGNLPAVHRLVNLFQQGGRELDIYNNLRQTPLHLAVITTLPSVVRLLVTAGASPMALDRHGQTAAHLACEHRSPTCLRALLDSAAPGTLDLEARNYDGLTALHVAVNTECQETVQLLLERGADIDAVDIKSGRSPLIHAVENNSLSMVQLLLQHGANVNAQMYSGSSALHSASGRGLLPLVRTLVRSGADSSLKNCHNDTPLMVARSRRVIDILRGKATRPASTSQPDPSPDRSANTSPESSSRLSSNGLLSASPSSSPSQSPPRDPPGFPMAPPNFFLPSPSPPAFLPFAGVLRGPGRPVPPSPAPGGS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20749 As Substrate

Site PTM Type Enzyme
K21 Ubiquitination
K34 Ubiquitination
S41 Phosphorylation
Y83 Phosphorylation
Y84 Phosphorylation
S122 Phosphorylation
K338 Methylation
S374 Phosphorylation
S386 Phosphorylation
S387 Phosphorylation
S402 Phosphorylation P49840 (GSK3A)
S406 Phosphorylation P49840 (GSK3A)
S448 Phosphorylation
S454 Phosphorylation

Research Backgrounds

Function:

Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation (By similarity).

PTMs:

Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation (By similarity).

Activated by phosphorylation.

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasm>Perinuclear region.
Note: Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of a complex consisting of the NF-kappa-B p52-p52 homodimer and BCL3. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Interacts with N4BP2, COPS5 and PIR. Interacts with CYLD (By similarity).

Research Fields

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

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