Phospho-MER/TYRO3 (Tyr753/Tyr685) Antibody - #AF8443
(1)
(1)
| Product: | Phospho-MER/TYRO3 (Tyr753/Tyr685) Antibody |
| Catalog: | AF8443 |
| Description: | Rabbit polyclonal antibody to Phospho-MER/TYRO3 (Tyr753/Tyr685) |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 110KD; 110kD,97kD(Calculated). |
| Uniprot: | Q12866 | Q06418 |
| RRID: | AB_2840500 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF8443, RRID:AB_2840500.
Fold/Unfold
c MER; c mer proto oncogene tyrosine kinase; c-mer; cMER; cmer protooncogene tyrosine kinase; Eyk; MER; MER receptor tyrosine kinase; MERK; MERPEN; Mertk; MERTK c-mer proto-oncogene tyrosine kinase; MERTK_HUMAN; MGC133349; nmf12; Nyk; Proto oncogene tyrosine protein kinase MER; Proto oncogene tyrosine protein kinase MER precursor; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK; RP38; STK kinase; Tyrosine-protein kinase Mer; Brt; BYK; DTK; Etk-2; protein tyrosine kinase 3; Rek; RSE; SKY; Tif; tyro3; TYRO3 protein tyrosine kinase; TYRO3_HUMAN; Tyrosine-protein kinase byk; Tyrosine-protein kinase DTK; Tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY; Tyrosine-protein kinase TIF;
Immunogens
Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. Highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver.
Q06418 TYRO3_HUMAN:Abundant in the brain and lower levels in other tissues.
- Q12866 MERTK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGPAPLPLLLGLFLPALWRRAITEAREEAKPYPLFPGPFPGSLQTDHTPLLSLPHASGYQPALMFSPTQPGRPHTGNVAIPQVTSVESKPLPPLAFKHTVGHIILSEHKGVKFNCSISVPNIYQDTTISWWKDGKELLGAHHAITQFYPDDEVTAIIASFSITSVQRSDNGSYICKMKINNEEIVSDPIYIEVQGLPHFTKQPESMNVTRNTAFNLTCQAVGPPEPVNIFWVQNSSRVNEQPEKSPSVLTVPGLTEMAVFSCEAHNDKGLTVSKGVQINIKAIPSPPTEVSIRNSTAHSILISWVPGFDGYSPFRNCSIQVKEADPLSNGSVMIFNTSALPHLYQIKQLQALANYSIGVSCMNEIGWSAVSPWILASTTEGAPSVAPLNVTVFLNESSDNVDIRWMKPPTKQQDGELVGYRISHVWQSAGISKELLEEVGQNGSRARISVQVHNATCTVRIAAVTRGGVGPFSDPVKIFIPAHGWVDYAPSSTPAPGNADPVLIIFGCFCGFILIGLILYISLAIRKRVQETKFGNAFTEEDSELVVNYIAKKSFCRRAIELTLHSLGVSEELQNKLEDVVIDRNLLILGKILGEGEFGSVMEGNLKQEDGTSLKVAVKTMKLDNSSQREIEEFLSEAACMKDFSHPNVIRLLGVCIEMSSQGIPKPMVILPFMKYGDLHTYLLYSRLETGPKHIPLQTLLKFMVDIALGMEYLSNRNFLHRDLAARNCMLRDDMTVCVADFGLSKKIYSGDYYRQGRIAKMPVKWIAIESLADRVYTSKSDVWAFGVTMWEIATRGMTPYPGVQNHEMYDYLLHGHRLKQPEDCLDELYEIMYSCWRTDPLDRPTFSVLRLQLEKLLESLPDVRNQADVIYVNTQLLESSEGLAQGSTLAPLDLNIDPDSIIASCTPRAAISVVTAEVHDSKPHEGRYILNGGSEEWEDLTSAPSAAVTAEKNSVLPGERLVRNGVSWSHSSMLPLGSSLPDELLFADDSSEGSEVLM
- Q06418 TYRO3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALRRSMGRPGLPPLPLPPPPRLGLLLAALASLLLPESAAAGLKLMGAPVKLTVSQGQPVKLNCSVEGMEEPDIQWVKDGAVVQNLDQLYIPVSEQHWIGFLSLKSVERSDAGRYWCQVEDGGETEISQPVWLTVEGVPFFTVEPKDLAVPPNAPFQLSCEAVGPPEPVTIVWWRGTTKIGGPAPSPSVLNVTGVTQSTMFSCEAHNLKGLASSRTATVHLQALPAAPFNITVTKLSSSNASVAWMPGADGRALLQSCTVQVTQAPGGWEVLAVVVPVPPFTCLLRDLVPATNYSLRVRCANALGPSPYADWVPFQTKGLAPASAPQNLHAIRTDSGLILEWEEVIPEAPLEGPLGPYKLSWVQDNGTQDELTVEGTRANLTGWDPQKDLIVRVCVSNAVGCGPWSQPLVVSSHDRAGQQGPPHSRTSWVPVVLGVLTALVTAAALALILLRKRRKETRFGQAFDSVMARGEPAVHFRAARSFNRERPERIEATLDSLGISDELKEKLEDVLIPEQQFTLGRMLGKGEFGSVREAQLKQEDGSFVKVAVKMLKADIIASSDIEEFLREAACMKEFDHPHVAKLVGVSLRSRAKGRLPIPMVILPFMKHGDLHAFLLASRIGENPFNLPLQTLIRFMVDIACGMEYLSSRNFIHRDLAARNCMLAEDMTVCVADFGLSRKIYSGDYYRQGCASKLPVKWLALESLADNLYTVQSDVWAFGVTMWEIMTRGQTPYAGIENAEIYNYLIGGNRLKQPPECMEDVYDLMYQCWSADPKQRPSFTCLRMELENILGQLSVLSASQDPLYINIERAEEPTAGGSLELPGRDQPYSGAGDGSGMGAVGGTPSDCRYILTPGGLAEQPGQAEHQPESPLNETQRLLLLQQGLLPHSSC
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q12866/Q06418 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N442 | N-Glycosylation | Uniprot | |
| T458 | Phosphorylation | Uniprot | |
| S543 | Phosphorylation | Uniprot | |
| Y549 | Phosphorylation | Uniprot | |
| K552 | Ubiquitination | Uniprot | |
| K591 | Ubiquitination | Uniprot | |
| S613 | Phosphorylation | Uniprot | |
| K622 | Ubiquitination | Uniprot | |
| S645 | Phosphorylation | Uniprot | |
| T699 | Phosphorylation | Uniprot | |
| S745 | Phosphorylation | Uniprot | |
| Y749 | Phosphorylation | Q12866 (MERTK) | Uniprot |
| S750 | Phosphorylation | Uniprot | |
| Y753 | Phosphorylation | Q12866 (MERTK) | Uniprot |
| Y754 | Phosphorylation | Q12866 (MERTK) | Uniprot |
| K761 | Ubiquitination | Uniprot | |
| K765 | Ubiquitination | Uniprot | |
| R775 | Methylation | Uniprot | |
| K856 | Ubiquitination | Uniprot | |
| Y872 | Phosphorylation | Q12866 (MERTK) | Uniprot |
| K923 | Ubiquitination | Uniprot | |
| Y929 | Phosphorylation | Uniprot | |
| S935 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S307 | Phosphorylation | Uniprot | |
| T427 | Phosphorylation | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| S466 | Phosphorylation | Uniprot | |
| K507 | Ubiquitination | Uniprot | |
| K526 | Ubiquitination | Uniprot | |
| S543 | Phosphorylation | Uniprot | |
| K582 | Ubiquitination | Uniprot | |
| Y681 | Phosphorylation | Uniprot | |
| S682 | Phosphorylation | Uniprot | |
| Y685 | Phosphorylation | Uniprot | |
| Y686 | Phosphorylation | Uniprot | |
| S818 | Phosphorylation | Uniprot | |
| Y828 | Phosphorylation | Uniprot | |
| S835 | Phosphorylation | Uniprot | |
| Y849 | Phosphorylation | Uniprot | |
| S869 | Phosphorylation | Uniprot |
PTMs - Q12866/Q06418 As Enzyme
Research Backgrounds
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
Autophosphorylated on Tyr-749, Tyr-753 and Tyr-754 in the activation loop allowing full activity. Autophosphorylated on Tyr-872 leading to recruitment of downstream partners of the signaling cascade such as PLCG2 (By similarity).
Membrane>Single-pass type I membrane protein.
Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. Highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver.
Interacts (upon activation) with TNK2; stimulates TNK2 autophosphorylation. Interacts (via N-terminus) with extracellular ligands LGALS3, TUB, TULP1 and GAS6 (By similarity). Interacts with VAV1 in a phosphotyrosine-independent manner.
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
(Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.
(Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.
Autophosphorylated.
Cell membrane>Single-pass type I membrane protein.
Abundant in the brain and lower levels in other tissues.
Monomer and homodimer. Interacts (via N-terminus) with extracellular ligands TULP1 and GAS6 (By similarity). Interacts with PIK3R1; this interaction increases PI3-kinase activity (By similarity).
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
References
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.