Phospho-HDAC7 (Ser486) Antibody - #AF8378

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Product: Phospho-HDAC7 (Ser486) Antibody
Catalog: AF8378
Description: Rabbit polyclonal antibody to Phospho-HDAC7 (Ser486)
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 105kDa; 103kD(Calculated).
Uniprot: Q8WUI4
RRID: AB_2840439

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(86%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-HDAC7 (Ser486) Antibody detects endogenous levels of HDAC7 only when phosphorylated at Ser486.
RRID:
AB_2840439
Cite Format: Affinity Biosciences Cat# AF8378, RRID:AB_2840439.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZP586J0917; FLJ99588; HD 7a; HD7; HD7a; HDAC 7; HDAC 7A; Hdac7; HDAC7_HUMAN; HDAC7A; Histone deacetylase 7; Histone deacetylase 7A; OTTHUMP00000202813; OTTHUMP00000202814;

Immunogens

Immunogen:
Uniprot:

Q8WUI4(HDAC7_HUMAN) >>Visit HPA database.

Gene(ID):
HDAC7(51564)
Sequence:
MDLRVGQRPPVEPPPEPTLLALQRPQRLHHHLFLAGLQQQRSVEPMRLSMDTPMPELQVGPQEQELRQLLHKDKSKRSAVASSVVKQKLAEVILKKQQAALERTVHPNSPGIPYRTLEPLETEGATRSMLSSFLPPVPSLPSDPPEHFPLRKTVSEPNLKLRYKPKKSLERRKNPLLRKESAPPSLRRRPAETLGDSSPSSSSTPASGCSSPNDSEHGPNPILGSEALLGQRLRLQETSVAPFALPTVSLLPAITLGLPAPARADSDRRTHPTLGPRGPILGSPHTPLFLPHGLEPEAGGTLPSRLQPILLLDPSGSHAPLLTVPGLGPLPFHFAQSLMTTERLSGSGLHWPLSRTRSEPLPPSATAPPPPGPMQPRLEQLKTHVQVIKRSAKPSEKPRLRQIPSAEDLETDGGGPGQVVDDGLEHRELGHGQPEARGPAPLQQHPQVLLWEQQRLAGRLPRGSTGDTVLLPLAQGGHRPLSRAQSSPAAPASLSAPEPASQARVLSSSETPARTLPFTTGLIYDSVMLKHQCSCGDNSRHPEHAGRIQSIWSRLQERGLRSQCECLRGRKASLEELQSVHSERHVLLYGTNPLSRLKLDNGKLAGLLAQRMFVMLPCGGVGVDTDTIWNELHSSNAARWAAGSVTDLAFKVASRELKNGFAVVRPPGHHADHSTAMGFCFFNSVAIACRQLQQQSKASKILIVDWDVHHGNGTQQTFYQDPSVLYISLHRHDDGNFFPGSGAVDEVGAGSGEGFNVNVAWAGGLDPPMGDPEYLAAFRIVVMPIAREFSPDLVLVSAGFDAAEGHPAPLGGYHVSAKCFGYMTQQLMNLAGGAVVLALEGGHDLTAICDASEACVAALLGNRVDPLSEEGWKQKPNLNAIRSLEAVIRVHSKYWGCMQRLASCPDSWVPRVPGADKEEVEAVTALASLSVGILAEDRPSEQLVEEEEPMNL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Chicken
100
Sheep
86
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WUI4 As Substrate

Site PTM Type Enzyme
S42 Phosphorylation
S49 Phosphorylation
K86 Ubiquitination
K96 Ubiquitination
T104 Phosphorylation
S109 Phosphorylation
Y114 Phosphorylation
T153 Phosphorylation
S155 Phosphorylation Q7KZI7 (MARK2) , Q15139 (PRKD1) , P27448 (MARK3) , Q13131 (PRKAA1) , O43318 (MAP3K7) , P98161 (PKD1)
K160 Sumoylation
K160 Ubiquitination
S181 Phosphorylation
S185 Phosphorylation
T247 Phosphorylation
T255 Phosphorylation
R277 Methylation
S283 Phosphorylation
T286 Phosphorylation
T301 Phosphorylation
S354 Phosphorylation
T356 Phosphorylation
S358 Phosphorylation Q13131 (PRKAA1) , Q15139 (PRKD1)
S364 Phosphorylation
T366 Phosphorylation
K382 Sumoylation
S391 Phosphorylation
S405 Phosphorylation
S464 Phosphorylation
S482 Phosphorylation
S486 Phosphorylation Q15139 (PRKD1)
S487 Phosphorylation
S493 Phosphorylation
S507 Phosphorylation
S508 Phosphorylation
T511 Phosphorylation
T515 Phosphorylation
Y524 Phosphorylation
S526 Phosphorylation
K530 Methylation
K530 Ubiquitination
S534 Phosphorylation
K571 Ubiquitination
S573 Phosphorylation
S595 Phosphorylation
K603 Acetylation
K603 Ubiquitination
K893 Ubiquitination
S903 Phosphorylation
S907 Phosphorylation

Research Backgrounds

Function:

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene. Positively regulates the transcriptional repressor activity of FOXP3. Serves as a corepressor of RARA, causing its deacetylation and inhibition of RARE DNA element binding. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response.

PTMs:

May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Phosphorylation at Ser-155 by MARK2, MARK3 and PRKD1 promotes interaction with 14-3-3 proteins and export from the nucleus. Phosphorylation at Ser-155 is a prerequisite for phosphorylation at Ser-181.

Subcellular Location:

Nucleus. Cytoplasm.
Note: In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and is due to its phosphorylation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, NCOR1, NCOR2, SIN3A, SIN3B, RBBP4, RBBP7, MTA1L1, SAP30 and MBD3. Interacts with the 14-3-3 protein YWHAE, MEF2A, MEF2B and MEF2C (By similarity). Interacts with KAT5 and EDNRA. Interacts with KDM5B. Interacts with ZMYND15 (By similarity). Interacts with PML (isoform PML-4). Interacts with FOXP3. Interacts with RARA.

Family&Domains:

The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.

Belongs to the histone deacetylase family. HD type 2 subfamily.

Research Fields

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

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