ILKAP Antibody - #AF0526
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0526, RRID:AB_2834319.
Fold/Unfold
DKFZp434J2031; FLJ10181; ILKAP; ILKAP_HUMAN; ILKAP2; ILKAP3; Integrin linked kinase associated phosphatase; Integrin linked kinase associated serine/threonine phosphatase 2C; Integrin linked kinase associated serine/threonine phosphatase; Integrin-linked kinase-associated serine/threonine phosphatase 2C; MGC4846; PP2C DELTA; Protein phosphatase 2c delta isozyme;
Immunogens
Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues.
- Q9H0C8 ILKAP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDLFGDLPEPERSPRPAAGKEAQKGPLLFDDLPPASSTDSGSGGPLLFDDLPPASSGDSGSLATSISQMVKTEGKGAKRKTSEEEKNGSEELVEKKVCKASSVIFGLKGYVAERKGEREEMQDAHVILNDITEECRPPSSLITRVSYFAVFDGHGGIRASKFAAQNLHQNLIRKFPKGDVISVEKTVKRCLLDTFKHTDEEFLKQASSQKPAWKDGSTATCVLAVDNILYIANLGDSRAILCRYNEESQKHAALSLSKEHNPTQYEERMRIQKAGGNVRDGRVLGVLEVSRSIGDGQYKRCGVTSVPDIRRCQLTPNDRFILLACDGLFKVFTPEEAVNFILSCLEDEKIQTREGKSAADARYEAACNRLANKAVQRGSADNVTVMVVRIGH
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H0C8 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| T81 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| K86 | Acetylation | Uniprot | |
| K95 | Acetylation | Uniprot | |
| K161 | Acetylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| C190 | S-Nitrosylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K204 | Methylation | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K210 | Acetylation | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| T263 | Phosphorylation | Uniprot | |
| Y265 | Phosphorylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| Y298 | Phosphorylation | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| R319 | Methylation | Uniprot | |
| R369 | Methylation | Uniprot | |
| R377 | Methylation | Uniprot |
Research Backgrounds
Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation.
Cytoplasm.
Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues.
Interacts with ILK. Specific association with ILK is independent of the catalytic activity of either partner.
Belongs to the PP2C family.
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