ZNF436 Antibody | Affinity Biosciences

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Western blot analysis of extracts from VERO, using ZNF436 Antibody.

AF0427 at 1/100 staining Mouse brain tissue by IHC-P.

Product:	ZNF436 Antibody
Catalog:	AF0427
Description:	Rabbit polyclonal antibody to ZNF436
Application:	WB IHC IF/ICC
Reactivity:	Human, Monkey
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	50kDa; 54kD(Calculated).
Uniprot:	Q9C0F3
RRID:	AB_2834317

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Monkey

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

ZNF436 Antibody detects endogenous levels of total ZNF436.

RRID:

AB_2834317
Cite Format: Affinity Biosciences Cat# AF0427, RRID:AB_2834317.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HGNC:20814; KIAA1710; Zfp46; Zinc finger protein 436; ZN436_HUMAN; ZNF; ZNF436;

Immunogens

Immunogen:

Uniprot:

Q9C0F3(ZN436_HUMAN) >>Visit HPA database.

Gene(ID):

ZNF436(80818)

Expression:

Q9C0F3 ZN436_HUMAN:

Expressed in fetal brain, heart, liver, spleen, bladder, lung, skin, skeletal muscle, stomach and pancreas.

Description:

ZNF436 May be a transcriptional repressor. Belongs to the krueppel C2H2-type zinc-finger protein family.

Sequence:

Q9C0F3 ZN436_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAATLLMAGSQAPVTFEDMAMYLTREEWRPLDAAQRDLYRDVMQENYGNVVSLDFEIRSENEVNPKQEISEDVQFGTTSERPAENAEENPESEEGFESGDRSERQWGDLTAEEWVSYPLQPVTDLLVHKEVHTGIRYHICSHCGKAFSQISDLNRHQKTHTGDRPYKCYECGKGFSRSSHLIQHQRTHTGERPYDCNECGKSFGRSSHLIQHQTIHTGEKPHKCNECGKSFCRLSHLIQHQRTHSGEKPYECEECGKSFSRSSHLAQHQRTHTGEKPYECNECGRGFSERSDLIKHYRVHTGERPYKCDECGKNFSQNSDLVRHRRAHTGEKPYHCNECGENFSRISHLVQHQRTHTGEKPYECNACGKSFSRSSHLITHQKIHTGEKPYECNECWRSFGERSDLIKHQRTHTGEKPYECVQCGKGFTQSSNLITHQRVHTGEKPYECTECEKSFSRSSALIKHKRVHTD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9C0F3 As Substrate

Q9C0F3

Site	PTM Type	Source
S10	Phosphorylation	Uniprot
K167	Sumoylation	Uniprot
T271	Phosphorylation	Uniprot
T273	Phosphorylation	Uniprot
T301	Phosphorylation	Uniprot
T355	Phosphorylation	Uniprot
T357	Phosphorylation	Uniprot
T385	Phosphorylation	Uniprot
T411	Phosphorylation	Uniprot
T413	Phosphorylation	Uniprot
Y418	Phosphorylation	Uniprot
K425	Sumoylation	Uniprot
T441	Phosphorylation	Uniprot
S456	Phosphorylation	Uniprot
K465	Methylation	Uniprot

Research Backgrounds

Q9C0F3_ZN436_HUMAN

Function:

May be a transcriptional repressor.

Subcellular Location:

Nucleus.

Tissue Specificity:

Expressed in fetal brain, heart, liver, spleen, bladder, lung, skin, skeletal muscle, stomach and pancreas.

Family&Domains:

Belongs to the krueppel C2H2-type zinc-finger protein family.

References

1). Expressions of ZNF436, β-catenin, EGFR, and CMTM5 in breast cancer and their clinical significances. EUROPEAN JOURNAL OF HISTOCHEMISTRY, 2021 (PubMed: 33478201) [IF=2.0]

Application: IHC Species: Human Sample: breast cancer and paracancerous tissues

Figure 1.Expression levels of ZNF436, β-catenin, EGFR and CMTM5 in breast cancer and paracancerous tissues. The expression levels of ZNF436, β-catenin and EGFR in breast cancer tissues were significantly higher than those in adjacent tissues (p<0.05); CMTM5 was highly expressed in adjacent tissues (p<0.05).

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ZNF436 Antibody - #AF0427