Product: Phospho-CGK2 (Ser126) Antibody
Catalog: AF8184
Description: Rabbit polyclonal antibody to Phospho-CGK2 (Ser126)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 87 KD; 87kD(Calculated).
Uniprot: Q13237
RRID: AB_2840246

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 100ul $350 In stock
 200ul $450 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
Phospho-CGK2 (Ser126) Antibody detects endogenous levels of CGK2 only when phosphorylated at Ser126.
RRID:
AB_2840246
Cite Format: Affinity Biosciences Cat# AF8184, RRID:AB_2840246.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CGK 2; cGK2; cGKII; cGMP dependent protein kinase 2; cGMP-dependent protein kinase 2; cGMP-dependent protein kinase II; KGP2_HUMAN; PRKG 2; PRKG2; PRKGR2; Protein kinase, cGMP dependent, type II; Type II cGMP dependent protein kinase;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q13237 KGP2_HUMAN:

Highly concentrated in brain, lung and intestinal mucosa.

Sequence:
MGNGSVKPKHSKHPDGHSGNLTTDALRNKVTELERELRRKDAEIQEREYHLKELREQLSKQTVAIAELTEELQNKCIQLNKLQDVVHMQGGSPLQASPDKVPLEVHRKTSGLVSLHSRRGAKAGVSAEPTTRTYDLNKPPEFSFEKARVRKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGRNYQQGSYIIKQGEPGNHIFVLAEGRLEVFQGEKLLSSIPMWTTFGELAILYNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEQYRNFLRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEEGSTFFILAKGKVKVTQSTEGHDQPQLIKTLQKGEYFGEKALISDDVRSANIIAEENDVACLVIDRETFNQTVGTFEELQKYLEGYVANLNRDDEKRHAKRSMSNWKLSKALSLEMIQLKEKVARFSSSSPFQNLEIIATLGVGGFGRVELVKVKNENVAFAMKCIRKKHIVDTKQQEHVYSEKRILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACVTEAFDYLHRLGIIYRDLKPENLILDAEGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDFSVDFWSLGILVYELLTGNPPFSGVDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTERLGNLKNGINDIKKHRWLNGFNWEGLKARSLPSPLQRELKGPIDHSYFDKYPPEKGMPPDELSGWDKDF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
90
Zebrafish
90
Chicken
90
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13237 As Substrate

Site PTM Type Enzyme
G2 Myristoylation
S11 Phosphorylation
S97 Phosphorylation Q13237 (PRKG2)
T109 Phosphorylation
S110 Phosphorylation Q13237 (PRKG2)
S114 Phosphorylation Q13237 (PRKG2)
S117 Phosphorylation Q13237 (PRKG2)
S126 Phosphorylation Q13237 (PRKG2)
T131 Phosphorylation
K157 Ubiquitination
K165 Sumoylation
K165 Ubiquitination
Y306 Phosphorylation
Y307 Phosphorylation
S431 Phosphorylation
S445 Phosphorylation Q13237 (PRKG2)
S448 Phosphorylation
K521 Methylation

PTMs - Q13237 As Enzyme

Substrate Site Source
P04626 (ERBB2) T686 Uniprot
P04792 (HSPB1) S78 Uniprot
P04792 (HSPB1) S82 Uniprot
P04792 (HSPB1) T143 Uniprot
P29474 (NOS3) T495 Uniprot
P29474 (NOS3) S633 Uniprot
P29474 (NOS3) S1177 Uniprot
P30307 (CDC25C) S216 Uniprot
P32929 (CTH) S377 Uniprot
P42261 (GRIA1) S863 Uniprot
P48436 (SOX9) S181 Uniprot
P49675 (STAR) S56 Uniprot
P49675 (STAR) S57 Uniprot
P49675 (STAR) S100 Uniprot
P49841 (GSK3B) S9 Uniprot
P50552 (VASP) S239 Uniprot
P53350 (PLK1) S137 Uniprot
P55211 (CASP9) S196 Uniprot
Q01970-1 (PLCB3) S26 Uniprot
Q01970-1 (PLCB3) S1105 Uniprot
Q03393 (PTS) S19 Uniprot
Q12778 (FOXO1) S319 Uniprot
Q13237 (PRKG2) S97 Uniprot
Q13237 (PRKG2) S110 Uniprot
Q13237 (PRKG2) S114 Uniprot
Q13237 (PRKG2) S117 Uniprot
Q13237 (PRKG2) S126 Uniprot
Q13237 (PRKG2) S445 Uniprot
Q14847-1 (LASP1) S61 Uniprot
Q14847 (LASP1) S146 Uniprot
Q15418 (RPS6KA1) S380 Uniprot
Q9UL51 (HCN2) S668 Uniprot

Research Backgrounds

Function:

Crucial regulator of intestinal secretion and bone growth (By similarity). Phosphorylates and activates CFTR on the plasma membrane. Plays a key role in intestinal secretion by regulating cGMP-dependent translocation of CFTR in jejunum (By similarity). Acts downstream of NMDAR to activate the plasma membrane accumulation of GRIA1/GLUR1 in synapse and increase synaptic plasticity. Phosphorylates GRIA1/GLUR1 at Ser-863 (By similarity). Acts as regulator of gene expression and activator of the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2 in mechanically stimulated osteoblasts. Under fluid shear stress, mediates ERK activation and subsequent induction of FOS, FOSL1/FRA1, FOSL2/FRA2 and FOSB that play a key role in the osteoblast anabolic response to mechanical stimulation (By similarity).

PTMs:

Myristoylation mediates membrane localization.

Subcellular Location:

Apical cell membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly concentrated in brain, lung and intestinal mucosa.

Subunit Structure:

Interacts with GRIA1/GLUR1.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Sensory system > Olfactory transduction.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Digestive system > Salivary secretion.

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