Product: Phospho-Mnk1 (Thr250) Antibody
Catalog: AF8169
Description: Rabbit polyclonal antibody to Phospho-Mnk1 (Thr250)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 51kDa; 51kD(Calculated).
Uniprot: Q9BUB5
RRID: AB_2840231

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 100ul $350 In stock
 200ul $450 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-Mnk1 (Thr250) Antibody detects endogenous levels of Mnk1 only when phosphorylated at Thr250.
RRID:
AB_2840231
Cite Format: Affinity Biosciences Cat# AF8169, RRID:AB_2840231.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MAP kinase interacting kinase 1; MAP kinase interacting serine/threonine kinase 1; MAP kinase signal integrating kinase 1; MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine-protein kinase 1; MAPK signal integrating kinase 1; MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1; mknk1; MKNK1_HUMAN; MNK 1; Mnk1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9BUB5 MKNK1_HUMAN:

Ubiquitous.

Sequence:
MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQGNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BUB5 As Substrate

Site PTM Type Enzyme
S39 Phosphorylation Q13177 (PAK2)
Y60 Phosphorylation
S68 Phosphorylation
S148 Phosphorylation
S191 Phosphorylation
S221 Phosphorylation
S226 Phosphorylation
S237 Phosphorylation
T250 Phosphorylation Q16539 (MAPK14)
T255 Phosphorylation Q16539 (MAPK14) , P28482 (MAPK1)
K332 Ubiquitination
K338 Ubiquitination
K353 Ubiquitination
T385 Phosphorylation P27361 (MAPK3)
S393 Phosphorylation
S413 Phosphorylation
S437 Phosphorylation
R455 Methylation
R459 Methylation
S460 Phosphorylation

PTMs - Q9BUB5 As Enzyme

Substrate Site Source
O43597 (SPRY2) S112 Uniprot
O43597 (SPRY2) S121 Uniprot
P06730 (EIF4E) S209 Uniprot
P09651 (HNRNPA1) S192 Uniprot
P09651 (HNRNPA1) S362 Uniprot
P09651 (HNRNPA1) S363 Uniprot
P09651 (HNRNPA1) S364 Uniprot
P23246 (SFPQ) S8 Uniprot
P23246 (SFPQ) S283 Uniprot
P47712 (PLA2G4A) S727 Uniprot
Q9NP80 (PNPLA8) S511 Uniprot

Research Backgrounds

Function:

May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.

PTMs:

Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1.

Subcellular Location:

Cytoplasm.

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases.

Family&Domains:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

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