Phospho-MAP3K2 (Ser520) Antibody - #AF8166

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Product: Phospho-MAP3K2 (Ser520) Antibody
Catalog: AF8166
Description: Rabbit polyclonal antibody to Phospho-MAP3K2 (Ser520)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 70kDa; 70kD(Calculated).
Uniprot: Q9Y2U5
RRID: AB_2840228

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-MAP3K2 (Ser520) Antibody detects endogenous levels of MAP3K2 only when phosphorylated at Ser520.
RRID:
AB_2840228
Cite Format: Affinity Biosciences Cat# AF8166, RRID:AB_2840228.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

M3K2_HUMAN; Map3k2; MAPK/ERK kinase kinase 2; MEK kinase 2; MEKK 2; MEKK2b; Mitogen activated protein kinase kinase kinase 2; Mitogen-activated protein kinase kinase kinase 2;

Immunogens

Immunogen:
Uniprot:

Q9Y2U5(M3K2_HUMAN) >>Visit HPA database.

Gene(ID):
MAP3K2(10746)
Sequence:
MDDQQALNSIMQDLAVLHKASRPALSLQETRKAKSSSPKKQNDVRVKFEHRGEKRILQFPRPVKLEDLRSKAKIAFGQSMDLHYTNNELVIPLTTQDDLDKAVELLDRSIHMKSLKILLVINGSTQATNLEPLPSLEDLDNTVFGAERKKRLSIIGPTSRDRSSPPPGYIPDELHQVARNGSFTSINSEGEFIPESMDQMLDPLSLSSPENSGSGSCPSLDSPLDGESYPKSRMPRAQSYPDNHQEFSDYDNPIFEKFGKGGTYPRRYHVSYHHQEYNDGRKTFPRARRTQGTSLRSPVSFSPTDHSLSTSSGSSIFTPEYDDSRIRRRGSDIDNPTLTVMDISPPSRSPRAPTNWRLGKLLGQGAFGRVYLCYDVDTGRELAVKQVQFDPDSPETSKEVNALECEIQLLKNLLHERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANILRDSTGNVKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVISGEGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPKLPPHVSDYTRDFLKRIFVEAKLRPSADELLRHMFVHYH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
92
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2U5 As Substrate

Site PTM Type Enzyme
S26 Phosphorylation
S109 Phosphorylation
S153 Phosphorylation
T158 Phosphorylation
S159 Phosphorylation
S163 Phosphorylation
S164 Phosphorylation
Y169 Phosphorylation
S239 Phosphorylation
Y240 Phosphorylation
S248 Phosphorylation
Y250 Phosphorylation
K257 Ubiquitination
K260 Methylation
T263 Phosphorylation
T283 Phosphorylation
T290 Phosphorylation
S297 Phosphorylation
S300 Phosphorylation
S302 Phosphorylation
T310 Phosphorylation
S311 Phosphorylation
S312 Phosphorylation
S314 Phosphorylation
S315 Phosphorylation
S331 Phosphorylation
T337 Phosphorylation
S344 Phosphorylation
S347 Phosphorylation
S349 Phosphorylation
K360 Ubiquitination
K385 Ubiquitination
K411 Ubiquitination
T432 Phosphorylation
K450 Ubiquitination
K485 Ubiquitination
S493 Phosphorylation
K498 Ubiquitination
S505 Phosphorylation
K506 Ubiquitination
S514 Phosphorylation
T516 Phosphorylation
S520 Phosphorylation
T522 Phosphorylation
T524 Phosphorylation
K581 Ubiquitination
K602 Ubiquitination

PTMs - Q9Y2U5 As Enzyme

Substrate Site Source
O14733 (MAP2K7) S271 Uniprot
O14733 (MAP2K7) T275 Uniprot

Research Backgrounds

Function:

Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.

PTMs:

Autophosphorylated.

Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Upon EGF stimulation, translocates into the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with PKN2; the interaction activates PKN2 kinase activity in a MAP3K2-independent kinase activity (By similarity). Self-associates. Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts (via the kinase catalytic domain) with STK38. Interacts with XIAP/BIRC4.

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

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