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$450 200ul

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  • Product Name
    Phospho-JAK3 (Tyr981) Antibody
  • Catalog No.
    AF8160
  • RRID
    AB_2840222
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA
  • Reactivity
    Human, Mouse, Rat, Monkey
  • Prediction
    Pig(100%), Bovine(91%), Horse(100%), Sheep(100%), Dog(100%)
  • UniProt
  • Mol.Wt
    (Observed)125kD; (Calculated)125kDa
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

EC 2.7.10.2; JAK 3; JAK L; JAK-3; Jak3; JAK3 HUMAN; JAK3_HUMAN; JAKL; Janus kinase 3 (a protein tyrosine kinase, leukocyte); Janus kinase 3; Janus Kinase3; L JAK; L-JAK; Leukocyte janus kinase; LJAK; Protein tyrosine kinase leukocyte; Tyrosine protein kinase JAK3; Tyrosine-protein kinase JAK3;

Applications:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse, Rat, Monkey

Predicted Reactivity:

Pig(100%), Bovine(91%), Horse(100%), Sheep(100%), Dog(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-JAK3 (Tyr981) Antibody detects endogenous levels of JAK3 only when phosphorylated at Tyr981.

RRID:

AB_2840222
Please cite this product as: Affinity Biosciences Cat# AF8160, RRID:AB_2840222.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human JAK3 around the phosphorylation site of Tyr981.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

JAK3

Molecular Weight:

Observed Mol.Wt.: (Observed)125kD.
Predicted Mol.Wt.: (Calculated)125kDa.

Subcellular Location:

Endomembrane system. Wholly intracellular, possibly membrane associated.

Tissue Specificity:

P52333 JAK3_HUMAN:
In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.

Sequence:
MAPPSEETPLIPQRSCSLLSTEAGALHVLLPARGPGPPQRLSFSFGDHLAEDLCVQAAKASGILPVYHSLFALATEDLSCWFPPSHIFSVEDASTQVLLYRIRFYFPNWFGLEKCHRFGLRKDLASAILDLPVLEHLFAQHRSDLVSGRLPVGLSLKEQGECLSLAVLDLARMAREQAQRPGELLKTVSYKACLPPSLRDLIQGLSFVTRRRIRRTVRRALRRVAACQADRHSLMAKYIMDLERLDPAGAAETFHVGLPGALGGHDGLGLLRVAGDGGIAWTQGEQEVLQPFCDFPEIVDISIKQAPRVGPAGEHRLVTVTRTDNQILEAEFPGLPEALSFVALVDGYFRLTTDSQHFFCKEVAPPRLLEEVAEQCHGPITLDFAINKLKTGGSRPGSYVLRRSPQDFDSFLLTVCVQNPLGPDYKGCLIRRSPTGTFLLVGLSRPHSSLRELLATCWDGGLHVDGVAVTLTSCCIPRPKEKSNLIVVQRGHSPPTSSLVQPQSQYQLSQMTFHKIPADSLEWHENLGHGSFTKIYRGCRHEVVDGEARKTEVLLKVMDAKHKNCMESFLEAASLMSQVSYRHLVLLHGVCMAGDSTMVQEFVHLGAIDMYLRKRGHLVPASWKLQVVKQLAYALNYLEDKGLPHGNVSARKVLLAREGADGSPPFIKLSDPGVSPAVLSLEMLTDRIPWVAPECLREAQTLSLEADKWGFGATVWEVFSGVTMPISALDPAKKLQFYEDRQQLPAPKWTELALLIQQCMAYEPVQRPSFRAVIRDLNSLISSDYELLSDPTPGALAPRDGLWNGAQLYACQDPTIFEERHLKYISQLGKGNFGSVELCRYDPLGDNTGALVAVKQLQHSGPDQQRDFQREIQILKALHSDFIVKYRGVSYGPGRQSLRLVMEYLPSGCLRDFLQRHRARLDASRLLLYSSQICKGMEYLGSRRCVHRDLAARNILVESEAHVKIADFGLAKLLPLDKDYYVVREPGQSPIFWYAPESLSDNIFSRQSDVWSFGVVLYELFTYCDKSCSPSAEFLRMMGCERDVPALCRLLELLEEGQRLPAPPACPAEVHELMKLCWAPSPQDRPSFSALGPQLDMLWSGSRGCETHAFTAHPEGKHHSLSFS

Research Background

Function:

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.

Post-translational Modifications:

Tyrosine phosphorylated in response to IL-2 and IL-4. Dephosphorylation of Tyr-980 and Tyr-981 by PTPN2 negatively regulates cytokine-mediated signaling (Probable).

Subcellular Location:

Endomembrane system>Peripheral membrane protein. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.

Subunit Structure:

Interacts with STAM2 and MYO18A (By similarity). Interacts with SHB.

Similarity:

Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

Research Fields

Research Fields:

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.(View pathway)
· Cellular Processes > Cell growth and death > Necroptosis.(View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.(View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer.(View pathway)
· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.(View pathway)
· Human Diseases > Immune diseases > Primary immunodeficiency.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.(View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation.(View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway.(View pathway)

Reference Citations:

1). Zhang F et al. Transcriptional regulation of voltage-gated sodium channels contributes to GM-CSF induced pain. J Neurosci 2019 Apr 23 (PubMed: 31015342) [IF=6.074]

2). Zhang F et al. Transcriptional Regulation of Voltage-Gated Sodium Channels Contributes to GM-CSF-Induced Pain. J Neurosci 2019 Jun 26;39(26):5222-5233 (PubMed: 31015342) [IF=6.074]

3). Chen L et al. Combination of gemcitabine and erlotinib inhibits recurrent pancreatic cancer growth in mice via the JAK-STAT pathway. Oncol Rep 2018 Mar;39(3):1081-1089 (PubMed: 29328487)

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Catalog Number :

AF8160-BP
(Blocking peptide available as AF8160-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Sheep
100%
Dog
100%
Horse
100%
Bovine
91%
Zebrafish
60%
Chicken
44%
Rabbit
0%
Xenopus
0%
High similarity Medium similarity Low similarity No similarity
P52333 as Substrate
Site PTM Type Enzyme
S5 Phosphorylation
T8 Phosphorylation
S15 Phosphorylation
S17 Phosphorylation
S20 Phosphorylation
K122 Ubiquitination
K157 Ubiquitination
K186 Ubiquitination
K237 Ubiquitination
K361 Ubiquitination
K388 Ubiquitination
K390 Ubiquitination
T391 Phosphorylation
S398 Phosphorylation
K482 Ubiquitination
S498 Phosphorylation
Y506 Phosphorylation
K534 Ubiquitination
Y536 Phosphorylation
S568 Phosphorylation
K624 Ubiquitination
K652 Ubiquitination
S663 Phosphorylation
K734 Ubiquitination
K748 Acetylation
S782 Phosphorylation
Y785 Phosphorylation P52333 (JAK3)
K823 Ubiquitination
K830 Ubiquitination
K855 Ubiquitination
K876 Ubiquitination
S880 Phosphorylation
Y886 Phosphorylation
Y904 Phosphorylation P52333 (JAK3)
Y929 Phosphorylation
S931 Phosphorylation
K935 Ubiquitination
Y939 Phosphorylation P52333 (JAK3)
S959 Phosphorylation
K972 Ubiquitination
K978 Ubiquitination
Y980 Phosphorylation P52333 (JAK3)
Y981 Phosphorylation P52333 (JAK3)
K1117 Ubiquitination
S1120 Phosphorylation
P52333 as PTM Enzyme
Substrate Site Source
O14939 (PLD2) Y415 Uniprot
O95644 (NFATC1) Y371 Uniprot
P23458 (JAK1) Y1034 Uniprot
P23458 (JAK1) Y1035 Uniprot
P29353-2 (SHC1) Y410 Uniprot
P29353-2 (SHC1) Y448 Uniprot
P42224 (STAT1) Y701 Uniprot
P42229 (STAT5A) Y694 Uniprot
P52333 (JAK3) Y785 Uniprot
P52333 (JAK3) Y904 Uniprot
P52333 (JAK3) Y939 Uniprot
P52333-1 (JAK3) Y980 Uniprot
P52333 (JAK3) Y981 Uniprot
Q15717 (ELAVL1) Y63 Uniprot
Q15717 (ELAVL1) Y68 Uniprot
Q15717 (ELAVL1) Y200 Uniprot
Q96LC7 (SIGLEC10) Y597 Uniprot
Q96LC7 (SIGLEC10) Y667 Uniprot
Q96LC7 (SIGLEC10) Y691 Uniprot
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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