AKAP14 Antibody - #AF0603

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Product: AKAP14 Antibody
Catalog: AF0603
Description: Rabbit polyclonal antibody to AKAP14
Application: WB IHC IF/ICC
Reactivity: Human
Mol.Wt.: 23kDa; 23kD(Calculated).
Uniprot: Q86UN6
RRID: AB_2834299

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
AKAP14 Antibody detects endogenous levels of total AKAP14.
RRID:
AB_2834299
Cite Format: Affinity Biosciences Cat# AF0603, RRID:AB_2834299.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A kinase (PRKA) anchor protein 14; A kinase anchor protein 14; A kinase anchor protein 28 kDa; A-kinase anchoring protein 28; AKAP14; AKAP28; PRKA14; Protein kinase A anchoring protein 14;

Immunogens

Immunogen:
Uniprot:

Q86UN6(AKA28_HUMAN) >>Visit HPA database.

Gene(ID):
AKAP14(158798)
Expression:
Q86UN6 AKA28_HUMAN:

Present in cilia (at protein level). Expressed in tissues containing axoneme-based organelles (cilia and/or flagella): trachea and testis. Highly expressed in airway cilia.

Description:
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The protein anchors PKA in ciliary axonemes and, in this way, may play a role in regulating ciliary beat frequency. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Sequence:
MSETQNSTSQKAMDEDNKAASQTMPNTQDKNYEDELTQVALALVEDVINYAVKIVEEERNPLKNIKWMTHGEFTVEKGLKQIDEYFSKCVSKKCWAHGVEFVERKDLIHSFLYIYYVHWSISTADLPVARISAGTYFTMKVSKTKPPDAPIVVSYVGDHQALVHRPGMVRFRENWQKNLTDAKYSFMESFPFLFNRV

PTMs - Q86UN6 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
T4 Phosphorylation
S7 Phosphorylation
S9 Phosphorylation
S185 Phosphorylation

Research Backgrounds

Function:

Binds to type II regulatory subunits of protein kinase A and anchors/targets them.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Present in cilia (at protein level). Expressed in tissues containing axoneme-based organelles (cilia and/or flagella): trachea and testis. Highly expressed in airway cilia.

Subunit Structure:

Binds to type II regulatory subunits (RII).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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