Phospho-Kv3.4/KCNC4 (Ser15) Antibody

pElisa

1/1

Product:	Phospho-Kv3.4/KCNC4 (Ser15) Antibody
Catalog:	AF8073
Description:	Rabbit polyclonal antibody to Phospho-Kv3.4/KCNC4 (Ser15)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.:	70KD; 70kD(Calculated).
Uniprot:	Q03721
RRID:	AB_2840136

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

Phospho-Kv3.4/KCNC4 (Ser15) Antibody detects endogenous levels of Kv3.4/KCNC4 only when phosphorylated at Ser15.

RRID:

AB_2840136
Cite Format: Affinity Biosciences Cat# AF8073, RRID:AB_2840136.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

Uniprot:

Q03721(KCNC4_HUMAN) >>Visit HPA database.

Gene(ID):

KCNC4(3749)

Sequence:

Q03721 KCNC4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MISSVCVSSYRGRKSGNKPPSKTCLKEEMAKGEASEKIIINVGGTRHETYRSTLRTLPGTRLAWLADPDGGGRPETDGGGVGSSGSSGGGGCEFFFDRHPGVFAYVLNYYRTGKLHCPADVCGPLFEEELTFWGIDETDVEPCCWMTYRQHRDAEEALDIFESPDGGGSGAGPSDEAGDDERELALQRLGPHEGGAGHGAGSGGCRGWQPRMWALFEDPYSSRAARVVAFASLFFILVSITTFCLETHEAFNIDRNVTEILRVGNITSVHFRREVETEPILTYIEGVCVLWFTLEFLVRIVCCPDTLDFVKNLLNIIDFVAILPFYLEVGLSGLSSKAARDVLGFLRVVRFVRILRIFKLTRHFVGLRVLGHTLRASTNEFLLLIIFLALGVLIFATMIYYAERIGARPSDPRGNDHTDFKNIPIGFWWAVVTMTTLGYGDMYPKTWSGMLVGALCALAGVLTIAMPVPVIVNNFGMYYSLAMAKQKLPKKRKKHVPRPAQLESPMYCKSEETSPRDSTCSDTSPPAREEGMIERKRADSKQNGDANAVLSDEEGAGLTQPLASSPTPEERRALRRSTTRDRNKKAAACFLLSTGDYACADGSVRKGTFVLRDLPLQHSPEAACPPTAGTLFLPH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Zebrafish

100

Chicken

100

Xenopus

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q03721 As Substrate

Q03721

Site	PTM Type	Enzyme	Source
S8	Phosphorylation	P05129 (PRKCG) , P05771 (PRKCB)	Uniprot
S9	Phosphorylation	P05129 (PRKCG) , P05771 (PRKCB)	Uniprot
S15	Phosphorylation	P05771 (PRKCB) , P05129 (PRKCG) , P17252 (PRKCA)	Uniprot
S21	Phosphorylation	P05129 (PRKCG) , P05771 (PRKCB) , P17252 (PRKCA)	Uniprot
T49	Phosphorylation		Uniprot
Y50	Phosphorylation		Uniprot
Y597	Phosphorylation		Uniprot

Research Backgrounds

Q03721_KCNC4_HUMAN

Function:

This protein mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

PTMs:

Phosphorylation of serine residues in the inactivation gate inhibits rapid channel closure.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Subunit Structure:

Homotetramer (Probable). Heterotetramer of potassium channel proteins (By similarity).

Family&Domains:

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Kv3.4/KCNC4 sub-subfamily.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-Kv3.4/KCNC4 (Ser15) Antibody - #AF8073