Product: Phospho-Gz alpha (Ser16) Antibody
Catalog: AF8062
Description: Rabbit polyclonal antibody to Phospho-Gz alpha (Ser16)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 41kDa; 41kD(Calculated).
Uniprot: P19086
RRID: AB_2840125

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 100ul $350 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-Gz alpha (Ser16) Antibody detects endogenous levels of Gz alpha only when phosphorylated at Ser16.
RRID:
AB_2840125
Cite Format: Affinity Biosciences Cat# AF8062, RRID:AB_2840125.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

G(x) alpha chain; GNAZ; GNAZ_HUMAN; Guanine nucleotide binding protein (G protein) alpha z polypeptide; Guanine nucleotide binding protein G(z) alpha subunit G(x) alpha chain; Guanine nucleotide binding protein G(z) subunit alpha; Guanine nucleotide-binding protein G(z) subunit alpha; Gz alpha; Gz-alpha; Human Gx alpha gene; Transducin alpha;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MGCRQSSEEKEAARRSRRIDRHLRSESQRQRREIKLLLLGTSNSGKSTIVKQMKIIHSGGFNLEACKEYKPLIIYNAIDSLTRIIRALAALRIDFHNPDRAYDAVQLFALTGPAESKGEITPELLGVMRRLWADPGAQACFSRSSEYHLEDNAAYYLNDLERIAAADYIPTVEDILRSRDMTTGIVENKFTFKELTFKMVDVGGQRSERKKWIHCFEGVTAIIFCVELSGYDLKLYEDNQTSRMAESLRLFDSICNNNWFINTSLILFLNKKDLLAEKIRRIPLTICFPEYKGQNTYEEAAVYIQRQFEDLNRNKETKEIYSHFTCATDTSNIQFVFDAVTDVIIQNNLKYIGLC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P19086 As Substrate

Site PTM Type Enzyme
S16 Phosphorylation Q05655 (PRKCD) , Q13153 (PAK1) , P17252 (PRKCA)
S27 Phosphorylation P17252 (PRKCA) , Q05655 (PRKCD)
T41 Phosphorylation
K51 Ubiquitination
S142 Phosphorylation
K189 Ubiquitination
S229 Phosphorylation
Y231 Phosphorylation
T263 Phosphorylation
S264 Phosphorylation
T341 Phosphorylation

Research Backgrounds

Function:

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

Subcellular Location:

Membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

G-proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with ADGRB2.

Family&Domains:

Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Research Fields

· Organismal Systems > Nervous system > Long-term depression.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.